Optimization of a Precolumn OPA Derivatization HPLC Assay for Monitoring of l-Asparagine Depletion in Serum during l-Asparaginase Therapy

Zhang, Mei; Zhang, Yong; Ren, Siqi; Zhang, Zunjian; Wang, Yongren; Song, Rui

doi:10.1093/chromsci/bmy053

Cited by 16 publications

(5 citation statements)

References 25 publications

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“…After incubation on ice for 30 min, the mixture was centrifuged at 16,000× g for 10 min at 4 °C, and the supernatant was diluted 1:5 with ice-cold borate buffer. Derivatization was performed as described by Zhang et al [ 23 ] by mixing 30 µl of the diluted sample with 50 µl of OPA derivatization reagent (prepared from 5 mg of OPA in 500 µl of ddH 2 O, 1 ml of borate buffer, and 27 µl of ethanethiol). Standard amino acid solutions were employed to determine the retention times of the individual amino acids.…”

Section: Methodsmentioning

confidence: 99%

The mitochondrial BCKD complex interacts with hepatic apolipoprotein E in cultured cells in vitro and mouse livers in vivo

Rueter

Rimbach

Treitz

et al. 2023

Cell. Mol. Life Sci.

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Background and aims Apolipoprotein E (APOE) is known for its role in lipid metabolism and its association with age-related disease pathology. The aim of the present work was to identify previously unknown functions of APOE based on the detection of novel APOE protein–protein interaction candidates. Approach and results APOE targeted replacement mice and transfected cultured hepatocytes expressing the human isoforms APOE3 and APOE4 were used. For 7 months, APOE3 and APOE4 mice were fed a high-fat and high-sugar diet to induce obesity, while a subgroup was subjected to 30% dietary restriction. Proteomic analysis of coimmunoprecipitation products from APOE mouse liver extracts revealed 28 APOE-interacting candidate proteins, including branched-chain alpha-keto acid dehydrogenase (BCKD) complex subunit alpha (BCKDHA) and voltage-dependent anion-selective channel 1 (VDAC1). The binding of APOE and BCKDHA was verified in situ by proximity ligation assay in cultured cells. The activity of the BCKD enzyme complex was significantly higher in obese APOE4 mice than in APOE3 mice, while the plasma levels of branched-chain amino acids and mTOR signalling proteins were not different. However, the protein–protein interaction with VDAC1 was strongly induced in APOE3 and APOE4 mice upon dietary restriction, suggesting a prominent role of APOE in mitochondrial function. Conclusions The protein–protein interactions of APOE with BCKDHA and VDAC1 appear to be of physiological relevance and are modulated upon dietary restriction. Because these are mitochondrial proteins, it may be suggested that APOE is involved in mitochondria-related processes and adaptation to hepatic energy demands.

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Section: Methodsmentioning

confidence: 99%

The mitochondrial BCKD complex interacts with hepatic apolipoprotein E in cultured cells in vitro and mouse livers in vivo

Rueter

Rimbach

Treitz

et al. 2023

Cell. Mol. Life Sci.

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“…O -Phthalaldehyde (OPA), a well-characterized derivatization reagent for amino acid analysis, has been used to analyze primary amines . OPA-based HPLC coupled with fluorescence detector (OPA-HPLC–FLD) based on the conversion of secondary to primary amines and the subsequent reaction with OPA has been developed to extend the OPA method to gizzerosine that contains neither fluorophore nor strong chromophore but secondary amines. − A precolumn OPA-HPLC–FLD study with three chromatographic purification steps showed that the fluorescence intensity was proportional to gizzerosine derivative doses in the range of 0.2–5.0 μg with a recovery rate of 95–102% . The derivatization way (precolumn, on-column, and postcolumn derivatization) determines the possibility of integrating the reaction into the chromatographic process and the degree of possible automation.…”

Section: Detection Of Gizzerosinementioning

confidence: 99%

Formation and Detection of Gizzerosine in Animal Feed Matrices: Progress and Perspectives

Jiao,

Qian

et al. 2024

J. Agric. Food Chem.

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Gizzerosine is responsible for gizzard erosion and black vomit, owing to excessive gastric acid secretion in poultry. It is a biogenic amine that forms during feed processing. Gizzerosine, a derivative of histamine, is a serious threat to animal feed safety and poultry production because it is more potent after ingestion and more harmful to poultry than histamine. The difficulty of obtaining gizzerosine and the lack of simple, rapid, and sensitive in vitro detection techniques have hindered studies on the effects of gizzerosine on gizzard health and poultry production. In this review, we evaluated the natural formation and the chemical synthesis methods of gizzerosine and introduced seven detection methods and their principles for analyzing gizzerosine. This review summarizes the issues of gizzerosine research and suggests methods for the future development of gizzerosine detection methods.

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“…The derivatization reagent was prepared by mixing 1 mL of OPA solution (10 mg/mL, dissolved in methanol) in 10 mL of borate buffer (consisting of 0.8 M borate buffer in KCl and 0.8 M NaOH with a final pH of 9.9) and 16 µL MCE. The derivatization reagent is stable within 9 days [29].…”

Section: Hplc Determination Of Lysine 241 Preparation Of Solutionsmentioning

confidence: 99%

The Effect of Terpenoid Compounds on the Formation of Advanced Glycation Endproducts (AGEs) in Model Systems

et al. 2022

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Background: Terpenoid compounds, despite their established antioxidant ability, are neglected as potential glycation regulators. Methods: In-vitro model systems of lysine (0.1 M) with glucose (0.1 M and 1 M) were incubated at 80 °C and 100 °C for 3 h in the presence of aniseed oil, thymol and linalool (2–8 μΜ). Color development, absorbance at UV-Vis (280 nm, 360 nm, 420 nm), fluorescence intensity (λexc = 370 nm, λemm = 440 nm) and lysine depletion (HPLC-FL) were measured to monitor the progress of the Maillard reaction. Response Surface Methodology was used to analyze the impact of the five experimental conditions on the glycation indices. Results: All terpenoid compounds promoted color development and did not affect lysine depletion. The choice of terpenoid compound impacted glycation at 280 nm, 360 nm and 420 nm (p < 0.02). The effect was stronger at lower temperatures (p < 0.002) and 0.1 M glucose concentrations (p < 0.001). Terpenoid concentration was important only at 360 nm and 420 nm (p < 0.01). No impact was seen for fluorescence intensity from the choice of terpenoid compounds and their dose (p = 0.08 and p = 0.44 respectively). Conclusion: Terpenoid compounds show both anti- and proglycative activity based on the incubation conditions. Thymol showed the largest antiglycative capacity, followed by aniseed oil and linalool. Maximal antiglycative capacity was seen at 0.1 M glucose, 2 μΜ terpenoid concentration, 80 °C and 1 h incubation.

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Optimization of a Precolumn OPA Derivatization HPLC Assay for Monitoring of l-Asparagine Depletion in Serum during l-Asparaginase Therapy

Cited by 16 publications

References 25 publications

The mitochondrial BCKD complex interacts with hepatic apolipoprotein E in cultured cells in vitro and mouse livers in vivo

The mitochondrial BCKD complex interacts with hepatic apolipoprotein E in cultured cells in vitro and mouse livers in vivo

Formation and Detection of Gizzerosine in Animal Feed Matrices: Progress and Perspectives

The Effect of Terpenoid Compounds on the Formation of Advanced Glycation Endproducts (AGEs) in Model Systems

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