Optimization of Agrobacterium Mediated Genetic Transformation in Paspalum scrobiculatum L. (Kodo Millet)

Bhatt, Ritika; Asopa, Prem Prakash; Jain, Rohit; Kothari-Chajer, Aditi; Kothari, S. L.; Kachhwaha, Sumita

doi:10.3390/agronomy11061104

Cited by 16 publications

(6 citation statements)

References 42 publications

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“…As a result, ascorbic acid, as a scavenging chemical, significantly enhanced GUS expression in, and shoot regeneration from, infected Chinese cabbage explants. Similarly, Bhatt et al (2021) reported maximum transformation efficiency was recorded in the presence of 2.5 mg/L ascorbic acid along with L-cysteine and silver nitrate in the pretreatment solution of Kodo millet callus.…”

Section: Discussionmentioning

confidence: 69%

“…When the antioxidants were used in the growing media, the tissue necrosis and browning can significantly reduce phenolic exudation in infected explants, and thereby increase the regeneration efficiency followed by improving the transformation efficiency. The addition of L-cysteine, ascorbic acid and silver nitrate in co-cultivation medium has also been revealed to considerably recover transformation efficiency in rice ( Enríquez-Obregón et al, 1999 ) and Kodo millet ( Bhatt et al, 2021 ). It has also been confirmed that L-cysteine improves the Agrobacterium -mediated transformation in soybean by reducing oxidative stress ( Olhoft et al, 2001 ).…”

Section: Introductionmentioning

confidence: 99%

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L-Cysteine Increases the Transformation Efficiency of Chinese Cabbage (Brassica rapa ssp. pekinensis)

et al. 2021

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Successful Agrobacterium-mediated transformations of Chinese cabbage have been limited owing to the plant’s recalcitrant nature, genomic background and explant necrosis upon infection, which hinders the transfer of T-DNA region into the Chinese cabbage. Consequently, in the current experiment, a stable Agrobacterium tumefaciens-mediated transformation method for Chinese cabbage cv. Kenshin established by employing important anti-oxidants in the co-cultivation and subsequent regeneration media. Four-day-old in vitro derived cotyledon explants were infected with A. tumefaciens strain GV3101 harboring the vector pCAMIBA1303. Cotyledon explants exposed to an Agrobacterium suspension (OD600 of approximately 0.6) for 10 min and then incubated for 3 days co-cultivation in Murashige and Skoog medium containing an L-cysteine + AgNO3 combination exhibited the highest β-glucuronidase (GUS) expression (94%) and explant regeneration efficiency (76%). After 3 days, the cotyledon explants were subjected to three selection cycles with gradually increasing hygromycin B concentrations (10 to 12 mg/L). The incorporation and expression of hptII in T0 transformed plants were verified by polymerase chain reaction and Southern blot analyses. These transgenic plants (T0) were fertile and morphologically normal. Using the present protocol, a successful transformation efficiency of 14% was achieved, and this protocol can be applied for genome editing and functional studies to improve Chinese cabbage traits.

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Section: Discussionmentioning

confidence: 69%

Section: Introductionmentioning

confidence: 99%

L-Cysteine Increases the Transformation Efficiency of Chinese Cabbage (Brassica rapa ssp. pekinensis)

et al. 2021

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“…The genome sequences of teff (VanBuren et al, 2020) and fonio millet were recently completed (Abrouk et al, 2020). In addition, the protocols for genetic transformation with agrobacterium and biolistic approaches in (Vetriventhan et al, 2020;Bhatt et al, 2021). These advances are expected to enhance the prospects of understanding the underlying genomics of small millets.…”

Section: Breeding Objectives and Prospects In Overcoming Constraintsmentioning

confidence: 99%

“…The genome sequences of teff ( VanBuren et al, 2020 ) and fonio millet were recently completed ( Abrouk et al, 2020 ). In addition, the protocols for genetic transformation with agrobacterium and biolistic approaches in finger millet, agrobacterium and biolistic methods in foxtail millet, biolistic methods in barnyard millet, agrobacterium-mediated approaches in teff and fonio, and agrobacterium-mediated methods in Kodo millet are now available ( Vetriventhan et al, 2020 ; Bhatt et al, 2021 ). These advances are expected to enhance the prospects of understanding the underlying genomics of small millets.…”

Section: Progress Of Crop Improvement In Small Milletsmentioning

confidence: 99%

Revitalization of small millets for nutritional and food security by advanced genetics and genomics approaches

et al. 2023

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Small millets, also known as nutri-cereals, are smart foods that are expected to dominate food industries and diets to achieve nutritional security. Nutri-cereals are climate resilient and nutritious. Small millet-based foods are becoming popular in markets and are preferred for patients with celiac and diabetes. These crops once ruled as food and fodder but were pushed out of mainstream cultivation with shifts in dietary habits to staple crops during the green revolution. Nevertheless, small millets are rich in micronutrients and essential amino acids for regulatory activities. Hence, international and national organizations have recently aimed to restore these lost crops for their desirable traits. The major goal in reviving these crops is to boost the immune system of the upcoming generations to tackle emerging pandemics and disease infestations in crops. Earlier periods of civilization consumed these crops, which had a greater significance in ethnobotanical values. Along with nutrition, these crops also possess therapeutic traits and have shown vast medicinal use in tribal communities for the treatment of diseases like cancer, cardiovascular disease, and gastrointestinal issues. This review highlights the significance of small millets, their values in cultural heritage, and their prospects. Furthermore, this review dissects the nutritional and therapeutic traits of small millets for developing sustainable diets in near future.

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“…After years of experiments, this perspective was completely changed. Numerous species, including cereals [179][180][181][182][183], gymnosperms [184][185][186][187], and algae [188] that were previously recalcitrant to A. tumefaciens infection can now be transformed. A. tumefaciens is not the only bacterium tested for the biological transformation of plants.…”

Section: Biological Systemsmentioning

confidence: 99%

Applications of In Vitro Tissue Culture Technologies in Breeding and Genetic Improvement of Wheat

Wijerathna-Yapa

Ramtekey

Ranawaka

et al. 2022

Plants

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Sources of new genetic variability have been limited to existing germplasm in the past. Wheat has been studied extensively for various agronomic traits located throughout the genome. The large size of the chromosomes and the ability of its polyploid genome to tolerate the addition or loss of chromosomes facilitated rapid progress in the early study of wheat genetics using cytogenetic techniques. At the same time, its large genome size has limited the progress in genetic characterization studies focused on diploid species, with a small genome and genetic engineering procedures already developed. Today, the genetic transformation and gene editing procedures offer attractive alternatives to conventional techniques for breeding wheat because they allow one or more of the genes to be introduced or altered into an elite cultivar without affecting its genetic background. Recently, significant advances have been made in regenerating various plant tissues, providing the essential basis for regenerating transgenic plants. In addition, Agrobacterium-mediated, biolistic, and in planta particle bombardment (iPB) gene delivery procedures have been developed for wheat transformation and advanced transgenic wheat development. As a result, several useful genes are now available that have been transferred or would be helpful to be transferred to wheat in addition to the current traditional effort to improve trait values, such as resistance to abiotic and biotic factors, grain quality, and plant architecture. Furthermore, the in planta genome editing method will significantly contribute to the social implementation of genome-edited crops to innovate the breeding pipeline and leverage unique climate adaptations.

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Optimization of Agrobacterium Mediated Genetic Transformation in Paspalum scrobiculatum L. (Kodo Millet)

Cited by 16 publications

References 42 publications

L-Cysteine Increases the Transformation Efficiency of Chinese Cabbage (Brassica rapa ssp. pekinensis)

L-Cysteine Increases the Transformation Efficiency of Chinese Cabbage (Brassica rapa ssp. pekinensis)

Revitalization of small millets for nutritional and food security by advanced genetics and genomics approaches

Applications of In Vitro Tissue Culture Technologies in Breeding and Genetic Improvement of Wheat

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