2022
DOI: 10.18805/ag.d-5557
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Optimization of Agrobacterium tumefaciens-mediated Transformation in Oxalis corniculata (L.)

Abstract: Background: Optimization of co-cultivation parameters during Agrobacterium-mediated transformation to Oxalis corniculata evaluated were bacterial density, infection period, acetosyringone (AS) concentration and co-cultivation temperature. Five fold transformation efficiency is achieved in transient gus gene expression after optimizing various parameters. The optimized conditions were Agrobacterium tumefaciens growth phase of A600nm 0.17, infection period of 30 min, addition of acetosyringone (AS) in co-cultiva… Show more

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