Optimization of Alkaline Protease Production by Solibacillus silvestris, Isolated from Gir Region of Gujarat

Abstract

Objectives: Microbes from Mangrove ecosystem have the ability to produce useful primary and secondary metabolites of biotechnological significance and hence the objective of this study is to optimize the production of bacterial alkaline protease from a Mangrove isolate Solibacillus silvestris. Methodology: The bacterium (named as Madhwad 10 −3 Summer Zobell) used in the present study was isolated from the soil collected from Madhwad, Gir, India in summer season and was identified using MALDI-TOF (Mass spectrometry). It had shown the highest alkaline protease enzyme activity amongst the other isolates and hence was further analyzed using different physical and chemical factors. Selected parameters i.e. Sucrose, Peptone, and Salinity were further used for the optimization using Response Surface Methodology (RSM). Findings: The bacterium (named as Madhwad 10 −3 Summer Zobell) was identified as Solibacillus silvestris. Among the physical parameters, the results proved that highest enzyme production can be obtained at pH 8.5, 40 °C of temperature, 48 h of incubation period, and agitation speed of 120 rpm while in the chemical parameters, Sucrose and Peptone with 0% salinity were found to give highest enzyme activity i.e. 221.7 U/ml and 246.7 U/ml, respectively. In the statistical analysis done using RSM, all the parameters found to be significant and gave the highest result of 258.750 U/ml at 2.5% Sucrose, 10% Peptone, and 0% Salinity level. Applications/improvements: The enzyme produced due to its efficiency in working at alkaline pH can be utilized in industries such as detergent and textile or in Bioremediation.

Remarkable Shift In Structural And Functional Properties of An Animal Charcoal Polluted Soil Accentuated By Inorganic Nutrients Amendment

“…It is therefore not surprising that they are making an impressive appearance in such a unique and little understood ecosystem as the soil of cottage industry producing Ponmo. It is equally of interest that just like the Anoxybacillus, Solibacillus are receiving attention as potential sources of value-added products, especially enzymes (Kharadi et al, 2020). Thus, the biostimulation with CFMM dramatically altered the community structure in favour of unique, rarely isolated and physiologically versatile strain which deserve to be further explored by isolating and properly characterizing them.…”

A novel fibrinolytic enzyme producer from mangrove soil sediments: Screening, isolation, strain improvement, and fermentation

“…Mangrove samples from Thuwal, a coastal location in Jeddah, Saudi Arabia have been used to isolate hydrolytic enzyme-producing bacteria which were further investigated for antifungal properties as well [28]. Similarly, an alkaline protease producing Solibacillus silvestris exhibiting a maximum of 258.75 U/mL alkaline protease activity, has also been reported from the Gir mangrove region in India [29].…”

Production of Alkaline Protease by a Novel Anaerobic Bacterium Isolated From a Municipal Anaerobic Treatment System

“…A wide variety of soil microorganisms (Aftab et al, 2006;Palsaniya et al, 2012;Sinha et al, 2013;Chauhan et al, 2020;Jadhav et al, 2020;Farooq et al, 2021;Hashmi et al, 2022) and strains isolated from mangrove ecosystems can produce proteases. Microbes isolated from mangrove ecosystems have a rapid growth rate in a limited space and are easy to genetically manipulate to produce new modified protease enzymes (Kharadi et al, 2020). Moreover, strains isolated from soda lakes and deserts can grow at an extremely alkaline pH and produce naturally stable alkaline enzymes.…”