Optimization of an Arterialized Venous Fasciocutaneous Flap in the Abdomen of the Rat

Casal, Diogo; Mota-Silva, Eduarda; Pais, Diogo; Iria, Inês; Videira, Paula A.; Tanganho, David; Alves, Sara; Mascarenhas-Lemos, Luís; Ferreira, José Martins; Ferraz-Oliveira, Mário; Vassilenko, Valentina; O’Neill, João Goyri

doi:10.1097/gox.0000000000001436

Cited by 5 publications

(8 citation statements)

References 29 publications

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“…Succinctly, a variant of the rat abdominal arterialized venous flap was used as model of ischemic flap (Fig. 2) 41,42 . This flap was transduced with an ex vivo infusion of a 100 µl solution containing approximately 4.7 × 10 9 plaque-forming-units of recombinant lentiviruses coding for Green Fluorescent Protein (GFP) or BD-2 and/or BD-3 43,44 .…”

Section: Methodsmentioning

confidence: 99%

“…In all animals, under a surgical operating microscope, a 5 cm long and 3 cm wide fasciocutaneous flap was raised on the left side of the rat’s abdomen 42 . Cranially, the flap was connected exclusively to the skeletonized lateral thoracic vein 46 .…”

Section: Methodsmentioning

confidence: 99%

“…Interrupted stitches were used for this anastomosis. The lateral thoracic vein was preserved cranially to ensure outflow from this arterialized venous flap 42 .…”

Section: Methodsmentioning

confidence: 99%

“…One hour after flap reperfusion, with the rat still under anesthesia, the rat’s abdomen was submitted to infrared thermography with a FLIR® E6 camera placed 25 cm above the abdomen. This evaluation intended to confirm the flap’s relative ischemia compared to the contralateral side 42 . Rats were placed on their backs for 10 min prior to this evaluation.…”

Section: Methodsmentioning

confidence: 99%

“…Rats were placed on their backs for 10 min prior to this evaluation. Thermographic measurements were made at a constant room temperature (22 °C) and humidity (50%) 42,47 .…”

Section: Methodsmentioning

confidence: 99%

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BD-2 and BD-3 increase skin flap survival in a model of ischemia and Pseudomonas aeruginosa infection

Casal

Iria

Ramalho

et al. 2019

Sci Rep

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The main aim of this work was to study the usefulness of human β-defensins 2 (BD-2) and 3 (BD-3), which are part of the innate immune system, in the treatment of infected ischemic skin flaps. We investigated the effect of transducing rat ischemic skin flaps with lentiviral vectors encoding human BD-2, BD-3, or both BD-2 and BD-3, to increase flap survival in the context of a P . aeruginosa infection associated with a foreign body. The secondary endpoints assessed were: bacterial counts, and biofilm formation on the surface of the foreign body. A local ischemic environment was created by producing arterialized venous flaps in the left epigastric region of rats. Flaps were intentionally infected by placing underneath them two catheters with 10 5 CFU of P . aeruginosa before the surgical wounds were hermetically closed. Flap biopsies were performed 3 and 7 days post-operatively, and the specimens submitted to immunohistochemical analysis for BD-2 and BD-3, as well as to bacterial quantification. Subsequently, the catheter segments were analyzed with scanning electron microscopy (SEM). Flaps transduced with BD-2 and BD-3 showed expression of these defensins and presented increased flap survival. Rats transduced with BD-3 presented a net reduction in the number of P . aeruginosa on the surface of the foreign body and lesser biofilm formation.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Interrupted stitches were used for this anastomosis. The lateral thoracic vein was preserved cranially to ensure outflow from this arterialized venous flap 42 .…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Rats were placed on their backs for 10 min prior to this evaluation. Thermographic measurements were made at a constant room temperature (22 °C) and humidity (50%) 42,47 .…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

BD-2 and BD-3 increase skin flap survival in a model of ischemia and Pseudomonas aeruginosa infection

Casal

Iria

Ramalho

et al. 2019

Sci Rep

Self Cite

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Algorithm for Automated Segmentation and Feature Extraction of Thermal Images

Poplavska¹,

Vassilenko²,

Poplavskyi³

et al. 2020

IFIP Advances in Information and Communication Technology

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Medical infrared thermal imaging techniques can provide the highquality images for monitoring and pre-clinical diagnostic of the diseases by showing the thermal abnormalities available in the body. Its biggest advantage is non-contact, non-invasive and very fast way of use. However, the incorrect interpretation of the thermal images via simple observation or manual analysis and detection approximate regions of interest lead to the numerous false positive results. The main objective of this research work is to develop an algorithm for automated image processing and analysis of the extracted features on thermal images for screening or pre-diagnosis of the diseases. This work presents the results of our previously developed Copyright algorithm applied to thermal images obtained from the patients with Axial Spondyloarthritis. This processing and analysis of the extracted features from thermal images can offer a novel quick and non-invasive tool for diagnosis and monitoring of rheumatoid diseases.

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Evaluation of Role of Arterialization of Venous Flaps in Abdomen in Rats

Aggarwal

Singh

Halageri

et al. 2021

Indian J Plast Surg

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Introduction Reconstruction forms the primary tenet in plastic surgery. Venous flaps are a known option but the survival is limited. Arterialization of venous flap can enhance its survival. While various techniques of arterialization of venous flaps are described, there are very few studies comparing them. Material and methods The current study was conducted among 34 rats weighing 160 to 200 grams. The rats were divided into four groups. Group I—islanded epigastric flap was raised with superficial caudal epigastric vessels as pedicle. Group II—arterialized flow through venous flap was raised with superficial caudal epigastric vein (SCEV) as afferent and lateral thoracic vein as drainage vein. Side-to-side anastomosis was done between femoral artery and vein, lateral to the origin of superficial caudal epigastric artery. Group III—after raising the flap, as in group II, femoral vein was ligated proximal to superficial caudal epigastric vessels. Group IV—an arterialized flow through venous flap was raised with superficial caudal epigastric vein as afferent and lateral thoracic vein as drainage vein. End-to-side anastomosis was done between femoral artery and superficial caudal epigastric vein. Animals that died before completion of the study were excluded. The color changes of flaps were noted. Flap survival was expressed as a percentage of the total flap surface area. The patency of anastomosis was seen on postoperative day 5. Results There was no total flap failure. On statical analysis, the flap survival area on day 5 between Group I and Group IV was not significant (p value 0.431). The survival area in Group I (78.85 ± 10.54%) was comparable to Group IV (65.71 ± 20.70%). Group II and III had poor results as compared with Group I. In four rats, thrombosis of arteriovenous anastomosis was noted with flap survival area of 30 to 33%. Conclusion It was noted that epigastric venous flaps with end-to-side anastomosis between femoral artery and superficial caudal epigastric vein (group IV) have survival area comparable to islanded flaps.

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Optimization of an Arterialized Venous Fasciocutaneous Flap in the Abdomen of the Rat

Abstract: Supplemental Digital Content is available in the text.

Cited by 5 publications

References 29 publications

BD-2 and BD-3 increase skin flap survival in a model of ischemia and Pseudomonas aeruginosa infection

BD-2 and BD-3 increase skin flap survival in a model of ischemia and Pseudomonas aeruginosa infection

Algorithm for Automated Segmentation and Feature Extraction of Thermal Images

Evaluation of Role of Arterialization of Venous Flaps in Abdomen in Rats

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