Optimization of an Economical Medium Composition For The Coculture of Clostridium Butyricum And Bacillus Coagulans

Abstract

Clostridium butyricum is a spore-forming probiotic existing in the intestines of humans and animals which can promote the enhancement of beneficial bacteria and maintain intestinal microecological balance. However, it is difficult to improve the production level of C. butyricum by conventional fermentation process. In this study, a co-fermentation process of C. butyricum DL-1 and Bacillus coagulans ZC2-1 was established to improve the viable counts and spore yield of C. butyricum, and the formula of coculture medium was optimized by flask fermentation. The results showed that the optimum medium composition is bran 10 g/L, corn steep powder 15 g/L, peptone 15 g/L, K2HPO4 1 g/L and MnSO4 0.5 g/L. Cultured stationarily in the optimal medium for 36 h, the number of viable bacteria of C. butyricum DL-1 reached 1.5×108 CFU/mL and the spore forming rate was 92.6%. The results revealed an economical and effective medium composition for the coculture of C. butyricum and B. coagulans. The co-fermentation process established in this study provides a new fermentation mode for the industrial production of other absolute anerobic bacteria.

Media Optimization and Partial Purification of Anti-Candida Compound of Probiotics Cultures

“…As a result, this study presents media optimization for the maximum anti-candida activity of previously identified probiotic anti-candida Bacillus cultures designated as MW3, MW9, MW19, and MW27 [22][23][24]. The partial purification and chemical characterization of an anti-candida molecule are also reported in this study.…”

“…Bacillus isolates designated as MW3, MW9, MW19, (Bacillus tequilensis) and MW27 (Bacillus subtilis), previously isolated from Indian traditional fermented batter of Meduwada, were used for further analysis of their anti-candida components [22][23][24]. These cultures were grown on Sabouraud dextrose agar (SDA) slants and stored in a refrigerator for further use.…”