Optimization of an effective extraction procedure for the analysis of microcystins in soils and lake sediments

Chen, Wei; Lin, Lu; Gan, Nanqin; Song, Lirong

doi:10.1016/j.envpol.2005.11.030

Cited by 75 publications

(62 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…For each experiment, samples were taken 3 times a week and lyophilized by frozen vacuum drier. 2 g of the dried soil sample was extracted with 0.1 M EDTA e 0.1 M Na 4 P 2 O 7 e 0.1% TFA and ultrasonicated (400 W, 10 min) (Chen et al, 2006), the resulting suspensions were centrifuged at 4000 Â g for 8 min, and the residues were extracted for two times. For each sample, the three supernatants were applied to preconditioned Sep-pak ODS cartridges and the eluted methanol solutions were reduced to 1ml under nitrogen flow, and then analyzed using an HPLC.…”

Section: Persistence Studiesmentioning

confidence: 99%

Sorption, degradation and mobility of microcystins in Chinese agriculture soils: risk assessment for groundwater protection

Chen

Song

Gan

et al. 2006

Environmental Pollution

Self Cite

124

View full text Add to dashboard Cite

Section: Persistence Studiesmentioning

confidence: 99%

Sorption, degradation and mobility of microcystins in Chinese agriculture soils: risk assessment for groundwater protection

Chen

Song

Gan

et al. 2006

Environmental Pollution

Self Cite

124

View full text Add to dashboard Cite

“…doi:10.1016/j.aca.2011.10.027 [11,15,17] and 0.1 M EDTA-0.1 M Na 4 P 2 O 7 [18]. However, a single solvent extraction is not considered sufficient for accurate quantification of many MCs in sediment samples owing to the lack of standard materials and inconsistent extraction efficiency, which could be strongly affected by both the type of sediments and the structure of MC variants [17].…”

Section: Introductionmentioning

confidence: 99%

Optimal strategies for determination of free/extractable and total microcystins in lake sediment

Wang

Xiao

et al. 2012

Analytica Chimica Acta

View full text Add to dashboard Cite

“…Previous results reported that mixtures of methanol and water extracted more efficiently compared to extraction with methanol and water, respectively [14], and acidified methanol led to the higher microcystins yields, since hydrophobic character of microcystins improves as the pH value decreases [15]. Wei et al [16] shows that microcystin-LR yield in acidified methanol was twice that obtained in methanol alone. Similar results also obtained in the present study.…”

Section: A Extraction Of Microcystins With Different Solvent Combinamentioning

confidence: 98%

Optimization of Microcystin Extraction for Their Subsequent Analysis by HPLC-MS/MS Method in Urban Lake Water

Qu¹,

Zhang²,

Jia³

et al. 2013

IJESD

View full text Add to dashboard Cite

Abstract-This paper represents to propose an effective extraction procedure for traditional toxin determination techniques in urban lake. Efficiency of each extraction solvent as well as other key parameters affecting extraction efficiency including ultrasonication time and extraction pH were evaluated and optimized. The present study results indicated that 40% acidified methanol (pH~3) with sonication 15~20min has been shown to be a rapid and efficient method for the routine analysis of a wide range of microcystins in water samples. Our study also suggested that analytical method based on HPLC-MS/MS with SPE techniques provides a simplified enrichment procedure and rich information for both quantization and identification effective tool for analysis of urban landscape water quality. The development of this method may facilitate the understanding of the best extraction method for the routine analysis of microcystins in water bodies. Moreover, it may be valuable for purification scale. Index Terms-Microcystin, extraction, HPLC-MS/MS, urban landscape water. I. INTRODUCTIONUrban landscape water in China are very often man-made ecosystem. They are very small and shallow. As an essential part of urban ecosystem, urban lake also play an important role in promoting socio-economic development, by regulating climate and providing a good place for recreational [1]- [2]. With increasing anthropogenic activities and excessive inputs of nitrogen and phosphorus to water environments, urban lake waters quality are extremely deteriorated in recent years. As a consequence, the appearance of Cyanobacteria (blue-green algae) can produce microsytins, frequently breakout in highly eutrophication lakes. It is considered to be an important water quality problem, implicating that a serious health problem in water supplies, both for livestock and human health [3]-[5].Microcystin is the most commonly synthesized toxin produced by toxin producing cyanobacteria species of Currently, there is a lack of understanding toxicology and risk assessment of urban lake Cyanobacterial toxins. For deeply study urban lake microcystins during algal blooms, optimization of extraction were conducted by HPLC-MS/MS approaches in our study for determination of microcystins from urban lake samples. II. MATERIALS AND METHODS A. ChemicalsAll the chemicals include HPLC-S grade acetonitrile, HPLC grade methanol and Trifluoroacetic acid (TFA) were purchased from Beijing Chemicals Ltd. Ultra-pure water was prepared on a Milli-Q Synthesis system (Millipore, France). microcystin LR, RR standards were bought from Beijing express Company. Sep-pak ODS cartridge (1000g, 12ml) was manufactured by Tianjin Alega Technologies. B. Sample Preparation for Microcystins ExtractionBefore the HPLC analyses, all water samples (1 L) were filtered on GF/C filters. Commercially available SPE fibers were purchased from Alega. Each fiber was eluted by 20ml methanol and pure water 20ml separately before extraction. To carry out the SPE extraction, water samples were extracted under diff...

show abstract

Optimization of an effective extraction procedure for the analysis of microcystins in soils and lake sediments

Cited by 75 publications

References 30 publications

Sorption, degradation and mobility of microcystins in Chinese agriculture soils: risk assessment for groundwater protection

Sorption, degradation and mobility of microcystins in Chinese agriculture soils: risk assessment for groundwater protection

Optimal strategies for determination of free/extractable and total microcystins in lake sediment

Optimization of Microcystin Extraction for Their Subsequent Analysis by HPLC-MS/MS Method in Urban Lake Water

Contact Info

Product

Resources

About