Optimization of Atmospheric Cold Plasma Treatment with Different Gases for Reduction of <i>Escherichia coli</i> in Wheat Flour

Lee, Jeongmin; Park, Seulki; Korber, Darren R.; Baik, Oon‐Doo

doi:10.4014/jmb.2203.03056

Cited by 7 publications

(2 citation statements)

References 36 publications

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“…This quantitative approach holds promise in enhancing our understanding of the fundamental mechanisms behind plasma–cell interactions. By enabling the assessment and quantification of plasma-induced effects, this methodology facilitates the fine-tuning of treatment dosages, a critical factor in optimizing therapeutic outcomes. − …”

Section: Results and Discussionmentioning

confidence: 99%

Multishell Nanophotonic Particles with Perovskite Core for Ratiometric Biochemical Sensing and Imaging

Nath,

Patrone,

Ray

2024

ACS Appl. Opt. Mater.

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Ultrabright nanomaterials are crucial for facilitating real-time imaging and biosensing applications, especially in deep tissues. Here, we present a strategy to utilize ultrabright perovskite nanocrystals (PNCs) that exhibit near-unity quantum yield and possess exceptionally high multiphoton absorption cross sections for biological imaging and sensing applications. Our approach includes synthesizing multishell coating for CsPbBr 3 PNCs by incorporating a silica (SiO 2 ) layer followed by biocompatible poly(lactic-co-glycolic acid) (PLGA), a Food and Drug Administration-approved copolymer, to effectively stabilize them in an aqueous environment. The resulting CsPbBr 3 @SiO 2 @PLGA nanoparticles exhibited remarkable stability in aqueous solution for >7 days. These highly luminescent PNC-based nanoparticles can be used for labeling cells and furthermore be readily converted into ratiometric nanosensors, allowing precise monitoring of various cellular processes. For a proof of concept, we incorporated a nitric oxide (NO)-responsive fluorescent dye, 4,5-diamino-rhodamine B, into the PLGA layer yielding a dual-emission nanoparticle with one emission peak constant and another responsive to the local NO concentration. The emission intensity ratio serves as a metric for quantifying NO concentrations, enabling detection of extracellular NO in the presence of live cells and monitoring NO concentrations during therapeutic processes. Furthermore, we also showcased the feasibility of employing two-photon microscopy to excite these nanosensors, for deep tissue imaging and sensing of the NO level.

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Section: Results and Discussionmentioning

confidence: 99%

Multishell Nanophotonic Particles with Perovskite Core for Ratiometric Biochemical Sensing and Imaging

Nath,

Patrone,

Ray

2024

ACS Appl. Opt. Mater.

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“…[34,35] Studies have demonstrated that the window of inhibition efficiency is determined by the concentration of intracellular ROS, which is determined by the parameters of CAP treatment, such as gas type, voltage, distance, or time. [36][37][38] In this work, we obtained a dose-response relationship of CAP treatment and U251 cell viability. Our results suggested that the apoptotic effects positively correlated with the dose of CAP.…”

Section: Discussionmentioning

confidence: 99%

Cold atmospheric‐pressure plasma selectively inhibits glioblastoma via DNA damage and AKT dephosphorylation in vitro and in vivo

Chen,

Qiao,

Liu

et al. 2024

Plasma Processes & Polymers

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In this work, the effects of cold atmospheric‐pressure plasma (CAP) on glioblastoma are evaluated comprehensively. After CAP treatment, U251 cell viability, migration, and invasion functions were inhibited, while an appropriate dose of CAP had no inhibitory effect on human brain glial cell line cells. Western blots indicated that expression of caspase‐3 was upregulated with ki‐67 expression downregulated. Moreover, mitochondrial membrane potential decreased, and energy metabolisms of U251 cells were influenced afterward. TUNEL assays and comet assays suggested the DNA damage of U251 cells after CAP treatment. Furthermore, as one of the DNA damage responses associated pathways, the AKT (AKT8 virus oncogene cellular homolog) signaling pathway was also indicated in the work. The findings raise great promise for clinical applications of CAP in glioblastoma treatments.

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Effects of plasma‐activated water and mild heating on Escherichia coli inactivation during wheat tempering and flour quality

Rivera,

Wang,

Doddabematti Prakash

et al. 2024

Cereal Chem

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Background and ObjectivesHeat treatment, as a pathogen reduction step, could compromise flour quality. Plasma‐activated water (PAW) is a novel pathogen reduction treatment for foods. Combining these treatments could improve pathogen inactivation during wheat tempering and maintain flour quality. This study evaluated the effects of PAW and mild heating on Escherichia coli inactivation during wheat tempering and wheat flour quality.FindingsE. coli was inoculated into wheat grains at 6.0 ± 0.1 log CFU/g. A 5‐log reduction was achieved after 6 h of tempering with PAW and heating (55°C), whereas 12 h were needed when using heating and deionized water (DI). Tempering with DI (control) and PAW alone only produced a 1‐log reduction after 24 h. The PAW and PAW + heat tempering treatments produced flours with comparable yield, physicochemical, dough rheology, and bread characteristics compared to the control treatment.ConclusionMild heating and PAW tempering demonstrated a synergistic effect as it produced greater E. coli reductions at shorter tempering times compared to the individual treatments. The hurdle approach used in this study did not compromise flour quality.Significance and NoveltyThe demonstrated hurdle approach can be a viable pathogen mitigation step in wheat milling which could help improve food safety of wheat‐based foods.

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Optimization of Atmospheric Cold Plasma Treatment with Different Gases for Reduction of Escherichia coli in Wheat Flour

Cited by 7 publications

References 36 publications

Multishell Nanophotonic Particles with Perovskite Core for Ratiometric Biochemical Sensing and Imaging

Multishell Nanophotonic Particles with Perovskite Core for Ratiometric Biochemical Sensing and Imaging

Cold atmospheric‐pressure plasma selectively inhibits glioblastoma via DNA damage and AKT dephosphorylation in vitro and in vivo

Effects of plasma‐activated water and mild heating on Escherichia coli inactivation during wheat tempering and flour quality

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