Optimization of Automated Sample Preparation for Vitamin D Determination on a Biomek i7 Workstation

Bach, A.; Fleischer, Heidi; Wijayawardena, Bhagya K.; Thurow, Kerstin

doi:10.1177/24726303211030291

Cited by 8 publications

(4 citation statements)

References 63 publications

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“…To ensure a high concentration rate, a maximum sample volume should be transferred to the sorbent bed without any protein aggregate contaminations. A total volume of 950 µL including a 200 µL serum sample was found to be most suitable (as previously shown in [66]). Volumes used for hydrolysis were determined based on very similar constraints.…”

Section: Automated Sample Preparationmentioning

confidence: 56%

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Automation System for the Flexible Sample Preparation for Quantification of Δ9-THC-D3, THC-OH and THC-COOH from Serum, Saliva and Urine

et al. 2022

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In the life sciences, automation solutions are primarily established in the field of drug discovery. However, there is also an increasing need for automated solutions in the field of medical diagnostics, e.g., for the determination of vitamins, medication or drug abuse. While the actual metrological determination is highly automated today, the necessary sample preparation processes are still mainly carried out manually. In the laboratory, flexible solutions are required that can be used to determine different target substances in different matrices. A suitable system based on an automated liquid handler was implemented. It has been tested and validated for the determination of three cannabinoid metabolites in blood, urine and saliva. To extract Δ9-tetrahydrocannabinol-D3 (Δ9-THC-D3), 11-hydroxy-Δ9-tetrahydrocannabinol (THC-OH) and 11-nor-9-carboxy-Δ9-tetrahydrocannabinol (THC-COOH) from serum, urine and saliva both rapidly and cost-effectively, three sample preparation methods automated with a liquid handling robot are presented in this article, the basic framework of which is an identical SPE method so that they can be quickly exchanged against each other when the matrix is changed. If necessary, the three matrices could also be prepared in parallel. For the sensitive detection of analytes, protein precipitation is used when preparing serum before SPE and basic hydrolysis is used for urine to cleave the glucuronide conjugate. Recoveries of developed methods are >77%. Coefficients of variation are <4%. LODs are below 1 ng/mL and a comparison with the manual process shows a significant cost reduction.

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Section: Automated Sample Preparationmentioning

confidence: 56%

“…For this purpose, the investment costs for the purchase of the equipment, the operating costs and the time required to perform the methods were determined. A more detailed description of the calculation method can be found in [66].…”

Section: Evaluation Under Economic Criteriamentioning

confidence: 99%

Automation System for the Flexible Sample Preparation for Quantification of Δ9-THC-D3, THC-OH and THC-COOH from Serum, Saliva and Urine

et al. 2022

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“…Based on the conditions mentioned, an automation concept for the metrological determination of vitamin D in blood samples was created [ 18 ]. These are individual samples that are available in 2-mL Eppendorf vials (decision criterion sample container).…”

Section: Discussionmentioning

confidence: 99%

“…Liquid handler-based systems have been described for the fully automated determination of vitamin D in blood samples, among other things [ 18 ]. The heart of the system is a liquid handler with 8 parallel, freely configurable channels for dosing the liquids and a second arm that is used with a gripper to transport the labware.…”

Section: Automation Strategiesmentioning

confidence: 99%

Strategies for automating analytical and bioanalytical laboratories

Thurow

2023

Anal Bioanal Chem

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Analytical measurement methods are used in different areas of production and quality control, diagnostics, environmental monitoring, or in research applications. If direct inline or online measurement methods are not possible, the samples taken have to be processed offline in the manual laboratory. Automated processes are increasingly being used to enhance throughput and improve the quality of results. In contrast to bioscreening, the degree of automation in (bio)analytical laboratories is still low. This is due in particular to the complexity of the processes, the required process conditions, and the complex matrices of the samples. The requirements of the process to be automated itself and numerous other parameters influence the selection of a suitable automation concept. Different automation strategies can be used to automate (bio)analytical processes. Classically, liquid handler-based systems are used. For more complex processes, systems with central robots are used to transport samples and labware. With the development of new collaborative robots, there will also be the possibility of distributed automation systems in the future, which will enable even more flexible automation and use of all subsystems. The complexity of the systems increases with the complexity of the processes to be automated. Graphical Abstract

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Liquid Handling in Laboratory Automation

2022

Devices and Systems for Laboratory Automation

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Optimization of Automated Sample Preparation for Vitamin D Determination on a Biomek i7 Workstation

Cited by 8 publications

References 63 publications

Automation System for the Flexible Sample Preparation for Quantification of Δ9-THC-D3, THC-OH and THC-COOH from Serum, Saliva and Urine

Automation System for the Flexible Sample Preparation for Quantification of Δ9-THC-D3, THC-OH and THC-COOH from Serum, Saliva and Urine

Strategies for automating analytical and bioanalytical laboratories

Liquid Handling in Laboratory Automation

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