Optimization of Bacitracin Production by Bacillus licheniformisB5

Ouled-Haddar, Houria; Aziz, Ghazi M.; Al-Gelawi, Majed Hussein

doi:10.3923/pjbs.2007.972.976

Cited by 12 publications

(4 citation statements)

References 15 publications

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“…In our study, the optimum temperature for antimicrobial compound production was observed to be 30 to 35 uC. Béahdy (1974) and Haddar et al (2007) observed production of bacitracin and other antibiotics by B. licheniformis (Zarei, 2012) at 37 uC, and it was also seen at 30 uC by Hosny et al (2011).…”

Section: Microbiologymentioning

confidence: 58%

See 1 more Smart Citation

Geochemical and mineralogical analysis of Kashmir Cave (SMAST), Buner, Pakistan, and isolation and characterization of bacteria having antibacterial activity

Zada¹,

Naseem²,

Lee³

et al. 2016

JCKS

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Bacterial strains having the ability to inhibit the growth of other bacteria were isolated from soil samples collected from Kashmir Smast (smast is Pushto for cave), Khyber Pakhtunkhwa, Pakistan. The study includes mineralogical and geochemical analyses of soil sample collected from the cave, so as to describe the habitat from which the microorganisms have been isolated. Total bacterial count of the soil sample was 5.25 3 10 4 CFU mL −1. Four bacterial isolates having activity against test organisms Micrococcus luteus, Klebsiella sp., Pseudomonas sp., and Staphylococcus aureus were screened out for further study. Two of the isolates were found to be Gram-positive and the other two Gram-negative. The four isolates showing antibacterial activity were identified as Serratia sp. KC1-MRL, Bacillus licheniformis KC2-MRL, Bacillus sp. KC3-MRL, and Stenotrophomonas sp. KC4-MRL on the basis of 16S rRNA sequence analysis. Although all isolates showed antibacterial activity, only Bacillus licheniformis KC2-MRL was selected for further study due to its large zone of inhibition. Antibacterial activity of B. licheniformis KC2-MRL was optimum when grown in nutrient broth adjusted to pH 5 and after 24 hours of incubation at 35 uC. The extracted antibacterial compound was stable at pH 5-7 and 40 uC when incubated for 1 hour. The strain was found resistant against cefotaxime (ctx). Atomic-absorption analysis of the soil sample collected from the cave showed high concentrations of calcium (332.938 mg kg ) compared to the control soil collected outside the cave. FTIR spectrum of the concentrated protein showed similarity to bacitracin. The antibacterial compound showed activity against both Gram-negative and positive test strains. Mineralogy of Kashmir Smast is diverse and noteworthy. Different geochemical classes identified by X-ray diffraction were nitrates, oxides, phosphates, silicates, and sulfates. Weathered cave limestone contributes notably to the formation of these minerals or compounds. FTIR spectroscopic analysis helped to identify minerals such as quartz, clinochlore, vermiculite, illite, calcite, and biotite.

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Section: Microbiologymentioning

confidence: 58%

“…Flickinger and Perlman (1979) reported pH 6.5 for the optimum production of antibiotics by B. licheniformis. Haddar et al (2007) found maximum bacitracin production rate (192 units/mL) at pH 7.5. A similar study was conducted by Gulahmadov et al (2006) that found antimicrobial activity was best at the wide pH range of 6-8 by Bacillus sp.…”

Section: Microbiologymentioning

confidence: 99%

Geochemical and mineralogical analysis of Kashmir Cave (SMAST), Buner, Pakistan, and isolation and characterization of bacteria having antibacterial activity

Zada¹,

Naseem²,

Lee³

et al. 2016

JCKS

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“…In recent decades, AMPs have drawn great interest from researchers due to their broad-spectrum activity, rapid lethality, and high cell selectivity [5]. For instance, Bacitracin can effectively inhibit the growth of Streptococcus pyogenes and Staphylococcus aureus, and it is currently utilized in clinical trials [6]. The cyclic lipopeptides polymyxin B and polymyxin E are used to treat Gram-negative infections, as the last line of defense against infections caused by multi-resistant Gram-negative bacteria [7].…”

Section: Therapeutic Peptides For Lung Infectionsmentioning

confidence: 99%

Therapeutic Peptides for Treatment of Lung Diseases: Infection, Fibrosis, and Cancer

Liu

et al. 2023

IJMS

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Various lung diseases endanger people’s health. Side effects and pharmaceutical resistance complicate the treatment of acute lung injury, pulmonary fibrosis, and lung cancer, necessitating the development of novel treatments. Antimicrobial peptides (AMPs) are considered to serve as a viable alternative to conventional antibiotics. These peptides exhibit a broad antibacterial activity spectrum as well as immunomodulatory properties. Previous studies have shown that therapeutic peptides including AMPs had remarkable impacts on animal and cell models of acute lung injury, pulmonary fibrosis, and lung cancer. The purpose of this paper is to outline the potential curative effects and mechanisms of peptides in the three types of lung diseases mentioned above, which may be used as a therapeutic strategy in the future.

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“…The polypeptides are the greatest importance of bacitracin, therefore it utilized in with anti microorganism agents [19]. For this reason, the cyclic and anionic AMP subtilosin, has been explained to apply as an anti microorganism activity against human pathogens [20].…”

Section: The Useful Functions To Amps Of the Bacillus Speciesmentioning

confidence: 99%

The Importance of Isolated and Purified Bacillus species as a Source of a Variety of Antimicrobial Substances

Alamoudi

2020

JPRI

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The rapid onset of resistance reduces the efficacy of most conventional antimicrobial drugs and is a general cause of concern for human well-being. Thus, there is a great demand for a continuous supply of novel antibiotics to combat this problem. Bacteria-derived antimicrobial peptides (AMPs) have long been used as food preservatives. Thus, antimicrobial agents are predominantly significant in limiting infectious diseases. Meanwhile, the development and dissemination of drug-resistant strains in pathogenic bacteria have begun to be a significant general health threat. Therefore, the lowest effectiveness of the present antibiotics in the direction of the administration was becoming prominent to the consequence of the discussion of new antibiotics. These considerations, Bacillus sp completely famous for making different metabolites of the possibility their utilization, thus, it is completed a search to isolate Bacillus sp for the action making of anti-microorganisms compounds. The Biosynthesis of Bacillus AMPs, extract, purifies, and identifies a bioactive compound by Bacillus sp. Furthermore, Bacillus-derived AMPs can be synthesized both ribosomal and non-ribosomal and can be classified according to peptide biosynthesis, structure, and molecular weight. The precise mechanism of action of these AMPs is not yet clear; however, the one proposed mechanism is that these AMPs kill bacteria by forming channels in and (or) disrupting the bacterial cell wall. Bacillus-derived AMPs have potential in the pharmaceutical industry, as well as the food and agricultural sectors. Here, we focus on Bacillus-derived AMPs as a novel alternative approach to antibacterial drug development.

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Optimization of Bacitracin Production by Bacillus licheniformisB5

Cited by 12 publications

References 15 publications

Geochemical and mineralogical analysis of Kashmir Cave (SMAST), Buner, Pakistan, and isolation and characterization of bacteria having antibacterial activity

Geochemical and mineralogical analysis of Kashmir Cave (SMAST), Buner, Pakistan, and isolation and characterization of bacteria having antibacterial activity

Therapeutic Peptides for Treatment of Lung Diseases: Infection, Fibrosis, and Cancer

The Importance of Isolated and Purified Bacillus species as a Source of a Variety of Antimicrobial Substances

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