Optimization of Baicalin, Wogonoside, and Chlorogenic Acid Water Extraction Process from the Roots of Scutellariae Radix and Lonicerae japonicae Flos Using Response Surface Methodology (RSM)

Li, Jichang; Rui, Wen; Sheng, Zunlai; Wu, Zhiyong; Chen, Chunli; Ishfaq, Muhammad

doi:10.3390/pr7110854

Cited by 3 publications

(1 citation statement)

References 25 publications

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“…The effect of interactions between factors on the response was plotted as a three-dimensional plot based on an established regression model ( Figure 3 and Figure 4 ). The three-dimensional plot is suitable for surface response analysis studies because it shows the possible independence of the factor with the response [ 39 ]. In Figure 3 a, the lutein contents were highest at 30 °C for 3 h and decreased with the change of the temperature and time, and especially, it decreased rapidly at 60 °C for 1 h and at 0 °C for 5 h. Figure 3 b shows that the lutein contents increased as the temperature approached 30 °C and as the solid–liquid ratio increased.…”

Section: Resultsmentioning

confidence: 99%

Optimization of Lutein Recovery from Tetraselmis suecica by Response Surface Methodology

et al. 2021

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Microalgae have been attracting attention as feedstock for biorefinery because they have various advantages, such as carbon fixation, high growth rate and high energy yield. The bioactive compounds and lutein contained in microalgae are known to be beneficial for human health, especially eye and brain health. In this study, in order to improve the recovery of bioactive extracts including lutein from Tetraselmis suecica with higher efficiency, an effective solvent was selected, and the extraction parameters such as temperature, time and solid loading were optimized by response surface methodology. The most effective solvent for lutein recovery was identified as 100% methanol, and the optimum condition was determined (42.4 °C, 4.0 h and 125 g/L biomass loading) by calculation of the multiple regression model. The maximum content of recovered lutein was found to be 2.79 mg/mL, and the ABTS radical scavenging activity (IC50) and ferric reducing antioxidant power (FRAP) value were about 3.36 mg/mL and 561.9 μmol/L, respectively. Finally, the maximum lutein recovery from T. suecica through statistical optimization was estimated to be 22.3 mg/g biomass, which was 3.1-fold improved compared to the control group.

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Section: Resultsmentioning

confidence: 99%

Optimization of Lutein Recovery from Tetraselmis suecica by Response Surface Methodology

et al. 2021

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Ultrasound-assisted Aqueous Two-Phase Extraction of Flavonoids from Scutellariae Radix and Evaluation of their Bioactivities In Vitro

Ge,

Zhao,

Ling

et al. 2024

CAC

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Background:: Scutellariae Radix, one of the most widely used herbs in Traditional Chinese Medicine, exhibits various biological activities due to its chemical components, which stand out for a number of flavonoids. In this study, Ultrasound-assisted aqueous two-phase extraction (UAATPE) was employed for the first time to obtain a high extraction rate and high purity of flavonoids from Scutellariae Radix. Methods:: The Box-Behnken response surface method (RSM) was utilized to optimize the extraction conditions with the application of the new aqueous two-phase system (ATPS) composed of ethanol and ammonium sulfate. The major influence factors, including ethanol concentration, ammonium sulfate concentration, liquid-to-solid ratio, sonication time, and extraction temperature, were investigated by the single-factor experiment. The compositional characterization of flavonoids was characterized with HPLC-UV. Scanning electron microscopy (SEM) was applied to research the surface morphology of raw material. Furthermore, the bioactivities of the extract obtained by UA-ATPE were studied in vitro. Results:: The optimal extraction conditions were as follows: the ethanol content was 26.12% (w/w), the ammonium sulfate content was 20.02% (w/w), the liquid-to-solid ratio was 40 mL/g, the sonication time was 5 min with the ultrasonic power of 250 W, and the operating process was performed at room temperature. Compared with the traditional extraction methods, UA-ATPE exhibited higher extraction efficiency and better extraction selectivity. The DPPH and ABTS radical scavenging tests showed that enriched products possessed strong antioxidant activity. Conclusion:: The study confirmed that the developed method of UA-ATPE could be used as an efficient, eco-friendly, and low-consumption method for the extraction and purification of flavonoids from Scutellariae Radix.

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Chemical Influence of Scutellaria baicalensis—Coptis chinensis Pair on the Extraction Efficiencies of Flavonoids and Alkaloids at Different Extraction Times and Temperatures

Kim

2023

Separations

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The Scutellaria baicalensis—Coptis chinensis pair is an herbal combination used for the treatment of various heat-related diseases. During the extraction process, two herbs can mutually influence the extraction efficiency of the chemical constituents contained in each herb. The concentrations of five flavonoids from S. baicalensis and seven alkaloids from C. chinensis were compared in paired or single hot-water extracts at different temperatures (80, 90, and 100 °C) and extraction times (60, 90, and 120 min). Temperature- and time-dependent increases in marker compound concentrations were observed in both paired and single extracts, with the exception of baicalin, berberine, and coptisine in the paired extracts at 100 °C. However, the extractions of the compounds in the paired and single extracts were affected differently by the extraction conditions. Furthermore, the concentrations of most marker compounds in single extracts were 1.09–44.13 times those in paired extracts. The contents of baicalin, wogonoside, coptisine, and berberine, known to be easily aggregated by the flavonoid–alkaloid complex, were changed by 0.024–0.764-fold in the paired extract. The effect of extraction temperature and time on the formation of the flavonoid–alkaloid complex was not significant. The extraction efficiency of the flavonoids and alkaloids can be affected by the pair of S. baicalensis—C. chinensis, which is a primary factor in the chemical modification of two herb-containing herbal extracts.

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Optimization of Baicalin, Wogonoside, and Chlorogenic Acid Water Extraction Process from the Roots of Scutellariae Radix and Lonicerae japonicae Flos Using Response Surface Methodology (RSM)

Cited by 3 publications

References 25 publications

Optimization of Lutein Recovery from Tetraselmis suecica by Response Surface Methodology

Optimization of Lutein Recovery from Tetraselmis suecica by Response Surface Methodology

Ultrasound-assisted Aqueous Two-Phase Extraction of Flavonoids from Scutellariae Radix and Evaluation of their Bioactivities In Vitro

Chemical Influence of Scutellaria baicalensis—Coptis chinensis Pair on the Extraction Efficiencies of Flavonoids and Alkaloids at Different Extraction Times and Temperatures

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