Optimization of Biomass and Fatty Acid Production by Aurantiochytrium sp. Strain 4W-1b

Nakazawa, Atsushi; Matsuura, Hiroshi; Kose, Ryoji; Ito, Kenichiro; Ueda, Mayumi; Honda, Daiske; Inouye, Isao; Kaya, Kunimitsu; Watanabe, Makoto M.

doi:10.1016/j.proenv.2012.05.006

Cited by 23 publications

(16 citation statements)

References 12 publications

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“…The growth rate of A. limacinum is very fast, production efficiency of hydrocarbons production is higher than Botryococcus braunii [3]. In general, the lipids content in A. limacinum is around 30-60wt% on a dry weight basis [4]. Commonly, chloroform, methanol, ethanol, and n-hexane were used as medium to extract lipids from marine tissue such as microalgae.…”

Section: Introductionmentioning

confidence: 99%

Economical Wet Extraction of Lipid from labyrinthula Aurantiochytrium limacinum by Using Liquefied Dimethyl Ether

Hoshino

Murakami

Wahyudiono

et al. 2016

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Abstract. Recently, a simple method for the extraction of lipids from wet biomass using liquefied dimethyl ether (DME) without drying, cell disruption, or heating was proposed. Here, the versatility of this method was evaluated for labyrinthula Aurantiochytrium limacinum (A. limacinum). The liquefied DME was passed through the extractor that filled by A. limacinum at different time intervals. The extraction of lipids from A. limacinum of moisture-rich microorganism was successfully achieved, the yield of lipid was 46.1 wt% of the dry weight of the sample. In comparison, the yields of lipid were 21.3 wt%, 43.6 wt% and 50.7 wt% when supercritical carbon dioxide (SCCO2), hexane-Soxhlet and Bligh-Dyer (BD) extraction methods were applied as extractants, respectively. However, the drying and cell-disruption process were required in SCCO2, hexane-Soxhlet, and BD extraction methods.

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Section: Introductionmentioning

confidence: 99%

Economical Wet Extraction of Lipid from labyrinthula Aurantiochytrium limacinum by Using Liquefied Dimethyl Ether

Hoshino

Murakami

Wahyudiono

et al. 2016

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“…DHA content in A. limacinum BUCHAXM 122 was higher than in Schizochytrium limacinum SR21 (14.72-18.38% TFA) [27], Aurantiochytrium sp. KRS101 (19.88% TFA) [28] and Aurantiochytrium 4W-1b (27.9% TFA) [29]. The DHA content was also higher than those reported for genus Thraustochytrium (T. striatum) (0.8-6% TFA) [30] and Thraustochytrium sp.…”

Section: Discussionmentioning

confidence: 67%

Characterization and PUFA production of Aurantiochytrium limacinum BUCHAXM 122 isolated from fallen mangrove leaves

Sirirak¹,

Suanjit²,

Powtongsook³

et al. 2020

ScienceAsia

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Aurantiochytrium limacinum BUCHAXM 122, a strain of thraustochytrid microorganism is potentially an alternative source of essential nutrients for commercial products. In this study, the strain was screened for its characterization and determination of polyunsaturated fatty acid (PUFA) production. Cells were isolated from fallen mangrove leaves, and grown in a glucose-yeast extract-peptone (GYP) medium. Their morphology, life cycle, biomass, fatty acid, extracellular enzymes and major elements were also investigated. Colonies occurred prominently on the fourth day of cultivation, appearing opaque white in color and containing large numbers of amoeboid cells. These thraustochytrids were observed to have a multi-stage life cycle, developing through zoospore, vegetative and zoosporangium stages with a life span of approximately 24 h. Zoospores developed into vegetative cells within 10-12 h and vegetative cells developed into zoosporangia, releasing zoospores, within approximately 10-15 h. Results showed that A. limacinum BUCHAXM 122 produced highest biomass at 108 h, with 23.85 ± 1.02 g/l dry weight. The isolates had high content of docosahexaenoic acid (DHA; 30.31 ± 5.88% of total fatty acids), indicating that they could serve as a fatty acid source for human consumption and aquaculture feeds. Major elemental analysis showed that cells contained 57% carbon, 29% oxygen, 9% hydrogen, 4% nitrogen and 1% sulfur. Extracellular degradative enzymes including protease, lipase, urease, α-glucosidase and phosphatase activities were also detected.

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“…Concerning the FAME profiles obtained in this study, they are in accordance with those previously described in the literature for Aurantiochytrium sp., with particularly high contents of myristic, palmitic, DPA and DHA [11,42,46,47] (Table 3). Interestingly, in the final validation trial, although WOM and IOM led to similar growth performances, higher biomass lipid and DHA contents were obtained in WOM.…”

Section: Discussionmentioning

confidence: 99%

Lab-Scale Optimization of Aurantiochytrium sp. Culture Medium for Improved Growth and DHA Production

Trovão¹,

Pereira

Costa³

et al. 2020

Applied Sciences

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Thraustochytrids have gained increasing relevance over the last decades, due to their fast growth and outstanding capacity to accumulate polyunsaturated fatty acids (PUFAs), particularly docosahexaenoic acid (DHA). In this context, the present work aimed to optimize the growth performance and DHA yields by improving the culture medium of Aurantiochytrium sp. AF0043. Accordingly, two distinct culture media were optimized: (i) an inorganic optimized medium (IOM), containing only monosodium glutamate and glucose as nitrogen and carbon sources, respectively; and (ii) an organic and sustainable waste-based optimized medium (WOM), containing corn steep powder and glycerol, added in fed-batch mode, as nitrogen and carbon sources, respectively. Overall, the lab-scale optimization allowed to increase the biomass yield 1.5-fold and enhance DHA content 1.7-fold using IOM. Moreover, WOM enabled a 2-fold increase in biomass yield and a significant improvement in lipid contents, from 22.78% to 31.14%. However, DHA content was enhanced almost 3-fold, from an initial content of 10.12% to 29.66% of total fatty acids contained in the biomass. Therefore, these results strongly suggest, not only that the production pipeline was significantly improved but also confirmed the potential use of Aurantiochytrium sp. AF0043 as a source of DHA.

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Optimization of Biomass and Fatty Acid Production by Aurantiochytrium sp. Strain 4W-1b

Cited by 23 publications

References 12 publications

Economical Wet Extraction of Lipid from labyrinthula Aurantiochytrium limacinum by Using Liquefied Dimethyl Ether

Economical Wet Extraction of Lipid from labyrinthula Aurantiochytrium limacinum by Using Liquefied Dimethyl Ether

Characterization and PUFA production of Aurantiochytrium limacinum BUCHAXM 122 isolated from fallen mangrove leaves

Lab-Scale Optimization of Aurantiochytrium sp. Culture Medium for Improved Growth and DHA Production

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