Optimization of Biosurfactant Lipopeptide Production from Bacillus subtilis S499 by Plackett-Burman Design

Jacques, Philippe; Hbid, Choukai; Destain, Jacqueline; Razafindralambo, Hary; Paquot, Michel; Pauw, Edwin De; Thonart, Philippe

doi:10.1385/abab:77:1-3:223

Cited by 121 publications

(79 citation statements)

References 20 publications

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“…The production of the phytohormone (IAA) and siderophores by Bacillus species has been investigated in many studies. The IAA stimulates the plant growth and siderophores chelate iron (Fe) and deprive the phytopathogenic fungi of it [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24]. Here, the Bacillus spp.…”

Section: Resultsmentioning

confidence: 99%

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Biocontrol and Plant Growth Promotion Characterization of Bacillus Species Isolated from Calendula officinalis Rhizosphere

et al. 2013

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The phenotypic and genotypic diversity of the plant growth promoting Bacillus genus have been widely investigated in the rhizosphere of various agricultural crops. However, to our knowledge this is the first report on the Bacillus species isolated from the rhizosphere of Calendula officinalis. 15 % of the isolated bacteria were screened for their important antifungal activity against Fusarium oxysporum, Botrytis cinerea, Aspergillus niger, Cladosporium cucumerinium and Alternaria alternata. The bacteria identification based on 16S r-RNA and gyrase-A genes analysis, revealed strains closely related to Bacillus amyloliquefaciens, B. velezensis, B. subtilis sub sp spizezenii and Paenibacillus polymyxa species. The electro-spray mass spectrometry coupled to liquid chromatography (ESI-LC MS) analysis showed that most of the Bacillus isolates produced the three lipopeptides families. However, the P. polymyxa (18SRTS) didn't produce any type of lipopeptides. All the tested Bacillus isolates produced cellulase but the protease activity was observed only in the B. amyloliquefaciens species (9SRTS). The Salkowsky colorimetric test showed that the screened bacteria synthesized 6-52 lg/ml of indole 3 acetic acid. These bacteria produced siderophores with more than 10 mm wide orange zones on chromazurol S. The greenhouse experiment using a naturally infested soil with Sclerotonia sclerotiorum showed that the B. amyloliquefaciens (9SRTS) had no significant (P [ 0.05) effect on the pre-germination of the chickpea seeds. However, it increased the size of the chickpea plants and reduced the stem rot disease (P \ 0.05).These results suggested that the Bacillus strains isolated in this work may be further used as bioinoculants to improve the production of C. officinalis and other crop systems.

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Section: Resultsmentioning

confidence: 99%

“…The Bacillus isolates were grown in the opt liquid medium described by Jacques et al [12], for 72 h at 30°C and agitated flasks (180 rpm). Spores concentration in Bacillus cultures was investigated using a thermal chock technique [8].…”

Section: Spore Yields Determinationmentioning

confidence: 99%

Biocontrol and Plant Growth Promotion Characterization of Bacillus Species Isolated from Calendula officinalis Rhizosphere

et al. 2013

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“…The strains CWBI-B1567 and CWBI-B1568 were grown at 30°C for 72 h in agitated flasks at 200 rpm in 350 ml of a liquid culture medium optimised for lipopeptide production (named Opt medium) as described by Jacques et al (1999). Cultures were centrifuged at 17,400 g for 20 min.…”

Section: Production and Purification Of Lipopeptidesmentioning

confidence: 99%

Antifungal activity and bioactive compounds produced by Bacillus mojavensis and Bacillus subtilis

Youcef-Ali¹,

Chaouche²,

Dehimat³

et al. 2014

Afr. J. Microbiol. Res.

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The soil bacteria, CWBI-B1567 and CWBI-B1568, isolated from arid regions and identified as Bacillus subtilis (accession number KC341751) and Bacillus mojavensis (accession number KC341749) respectively, were screened and evaluated for their antifungal activity against Candida albicans, one of the most important human fungal pathogens. In vitro assay with the antagonists and their cell-free culture supernatants on agar plates showed that the two Bacillus strains effectively inhibited growth of the yeast. The capacity of these Bacillus strains to produce the cell-wall degrading enzymes was further studied. B. subtilis and B. mojavensis are able to produce cellulase and protease, but not chitinase. Bioactive molecules were produced by B. subtilis CWBI-B1567 and B. mojavensis CWBI-B1568 in a liquid culture medium optimised for lipopeptide production. The antifungal activity was equally demonstrated by testing the resulting supernatants and lipopeptide-enriched extracts. The electro-spray mass spectrometry coupled to liquid chromatography (ESI-LC-MS) analysis showed that both the B. subtilis and the B. mojavensis produced surfactin and iturin. However the fengycin group was produced only by B. mojavensis. Through this study, we have demonstrated that B. subtilis and B. mojavensis have a strong antifungal activity especially against C. albicans. This growth inhibition is probably due to the production of cell-wall degrading enzymes and different families of lipopeptide including iturins, fengycins and surfactins.

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“…This, make the production of bacteria belonging to the Bacillus genus, at the industrial level, realizable and profitable. In this work, the strain (4RH) was characterized by a high sporulation yield up to 22±0.86 X 10 8 spores/ml (Table 1), estimated after three days of incubation in optimum medium and under culture conditions, as described by Jacques et al (1999). This sporulation yield is considered important, in comparison with the highest reported values estimated in a bioreactor culture conditions as established by Monteiro et al (2010Monteiro et al ( , 2014.…”

Section: Resultsmentioning

confidence: 62%

“…The Bacillus isolate 4RH was grown in the optimum liquid medium, prepared as described by Jacques et al (1999), for 72 h of incubation, under a temperature of 30°C and an agitation of 180 rpm. The culture obtained was treated by heat chock at 80°C for 12 min, followed by immediate cooling to room temperature, in cooled water.…”

Section: Sporulation Assessmentmentioning

confidence: 99%

In vitro biocontrol and biofertilization features study of a Bacillus amyloliquefaciens (4RH) strain isolated from a hot spring soil in Algeria

Kaki¹,

Ali²,

Milet³

et al. 2017

Afr. J. Microbiol. Res.

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Several Bacillus strains were isolated from "Ouled Yelass" hot spring soil, located in Setif city (Eastern Algeria). Three isolates of them coded (4RH), (14 RH), and (Set-oxy), were screened for their ability to inhibit the growth of some phytopathogenic fungi, such as Fusarium oxysporium, Botrytis cinerea, Aspergillus niger, Cladosporium cucumerinium and Alternaria alternata. The molecular identification of the strain (4RH) based on the 16S-DNA and gyrase-A genes sequences analysis, showed that it is closely related (99.9%) to the Bacillus amyloliquefaciens species. This bacterium was characterized by a high sporulation yield reaching 22±0.86 × 10 8 spores/ml and had important inhibition rates up to 80 and 70%, against F. oxysporium and B. cinerea, respectively. The bacterium (4RH) was able to produce cell wall degrading enzymes of cellulase and protease, in contrast to chitinase activity which was negative. Furthermore, it produced the three lipopeptides families, which are, iturins A (C14; C15), fengycin A (C14, C15, C16, C17, and C18), and surfactin (C12, C13, C14, C15, C16). Interestingly, LC-MS profiles of 80% acetonitrile fengycin extract of the (4RH) strain, showed the presence of some new molecular ions (MH+) with masses different, but near to conventional fengycin variants, which correspond to new variants described for the first time in this work. The isolate (4RH) produced 5 µg/ml of the phytohormone (IAA), on TGE medium and sidérophores with more than 10 mm of yellow-orange zones, on CAS medium. To conclude, B. amyloliquefaciens (4RH) strain possessed interesting biocontrol and biofertilization characteristics, in vitro, in addition to its high sporulation yield, which make it a potential agent for future biopesticide that could be efficient in the integrate pest management and organic agricultural production systems.

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Optimization of Biosurfactant Lipopeptide Production from Bacillus subtilis S499 by Plackett-Burman Design

Cited by 121 publications

References 20 publications

Biocontrol and Plant Growth Promotion Characterization of Bacillus Species Isolated from Calendula officinalis Rhizosphere

Biocontrol and Plant Growth Promotion Characterization of Bacillus Species Isolated from Calendula officinalis Rhizosphere

Antifungal activity and bioactive compounds produced by Bacillus mojavensis and Bacillus subtilis

In vitro biocontrol and biofertilization features study of a Bacillus amyloliquefaciens (4RH) strain isolated from a hot spring soil in Algeria

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