Optimization of butanol production from corn straw hydrolysate by Clostridium acetobutylicum using response surface method

Abstract

Butanol is a new kind of very potential biofuels. Enzymatic hydrolysis of corn stalk was utilized in this study to produce butanol by Clostridium acetobutylicum CICC 8008. Plackett-Burman (P-B) design and Central Composite Design (CCD) were adopted to screen crucial factors during fermentation as well as the optimization of experimental conditions. The result demonstrated that among the seven factors, namely, Yeast extract, (NH 4 ) 2 SO 4 , KH 2 PO 4 , MgSO 4 , FeSO 4 , CuSO 4 and CaCO 3 , only CaCO 3 was selected as the most critical factor. The optimization experiment results for CaCO 3 usage, temperature and reaction time by CCD were determined to be 5.04 g/L, 35°C and 70 h, respectively. A corresponding mathematical model was established to predict the fermentation experiment and maximum butanol yield of 6.57 g/L was acquired. The result of verification experiment under the optimum conditions showed that 6.20 g/L was the maximum butanol yield. This demonstrated that statistical method was a powerful tool for the optimization of butanol production from enzymatic hydrolysis of corn stalk. Lin Y S, Wang J, Wang X M, et al. Optimization of butanol production from corn straw hydrolysate by Clostridium acetobutylicum using response surface method.

Microbial inhibitors: formation and effects on acetone-butanol-ethanol fermentation of lignocellulosic biomass

“…Although Clostridium species can grow in different media, C. beijerinckii can grow faster at slightly higher temperatures compared to C. acetobutylicum. Different studies suggest that the optimum average temperatures for C. beijerinckii and C. acetobutylicum are 36.5 and 35.6°C, respectively Ennis and Maddox 1985;Ezeji et al 2007a;Gao et al 2012;Guo et al 2012;Lin et al 2011;Fig. 1 Composition and ABE yield of the most common agricultural residues.…”

Can Microbially Derived Advanced Biofuels Ever Compete with Conventional Bioethanol? A Critical Review

“…Researchers have examined a range of biomass sources including corn stover, corn fiber, barley straw, and switch grass, as well as a variety of pretreatment conditions to assess their influence on n-butanol production (Parekh and Blaschek 1999;Parekh and Formanek 1999;Qureshi et al 1999;Qureshi et al 2006;Ezeji et al 2007;Zhang et al 2009;Qureshi et al 2010;Lin et al 2011;Mu et al 2011;Guo et al 2012Guo et al , 2013. Sugar concentrations ranged from 31 to 58 g/L with solvent titers ranging from 2 to 26 g/L (Parekh and Blaschek 1999;Parekh and Formanek 1999;Qureshi et al 1999Qureshi et al , 2006Ezeji et al 2007;Zhang et al 2009;Qureshi et al 2010;Lin et al 2011;Mu et al 2011;Guo et al 2012Guo et al , 2013. The highest yield reported was 0.44 g/g (Qureshi et al 2006(Qureshi et al , 2010, and the highest productivity was 5 g/L/h ).…”

Fermentable sugars and microbial inhibitors formation from two-stage pretreatment of corn stalk with variation in particle size and severity factor

“…The average activity of the enzyme was 100 filter paper units per ml (FPU/ml). The cellulase was loaded at 4.5 FPU/g glucan (Lin et al, 2011) and hydrolysis was performed at 45 ± 1°C, 100 rpm in a rotary shaker (FLY-2102C, Shanghai Shenxian Thermostatic Equipment Factory, China). All the tests were run in triplicate.…”

“…Liquid samples were taken periodically for reducing sugar analysis. The samples were filtered by eight layers of gauze and centrifuged at 8000 rpm for 10 min (Lin et al, 2011).…”