Optimization of Caesalpinia sappan L. heartwood extraction procedure to obtain the highest content of brazilin and greatest antibacterial activity

Settharaksa, Sukanya; Monton, Chaowalit; Charoenchai, Laksana

doi:10.1016/j.joim.2019.05.003

Cited by 29 publications

(22 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…TCM has a long history and had attracted lots of researchers’ interests. Nowadays, more efforts were focused on the functionally active chemical compounds isolated from TCM drugs 33,34 . We had a long‐term interest in combining TCM and gene therapy, and have confirmed that several TCM chemical compounds including shikonin, celastrol, and pristimerin can be introduced to improve the hepatocytes of transduction efficiency mediated by rAAV2, rAAV3 or rAAV8 23,24 .…”

Section: Discussionmentioning

confidence: 88%

“…Nowadays, more efforts were focused on the functionally active chemical compounds isolated from TCM drugs. 33,34 We had a long-term interest in combining TCM and gene therapy, and have confirmed that several TCM chemical compounds including shikonin, celastrol, and pristimerin can be introduced to improve the hepatocytes of transduction efficiency mediated by F I G U R E 5 Depletion of ROS reduced shikonin-enhanced rAAV6 transduction efficiency in hematopoietic cells. A and B, K562 cells were treated with 300 nM shikonin for 72 hours.…”

mentioning

confidence: 93%

See 1 more Smart Citation

Mechanism for enhanced transduction of hematopoietic cells by recombinant adeno‐associated virus serotype 6 vectors

Chen

Jia

et al. 2020

FASEB j.

View full text Add to dashboard Cite

Hematopoietic gene delivery, such as hematopoietic stem/progenitor cells (HSPCs), is a promising treatment for both inherited and acquired diseases, such as hemophilia. Recently, a combined strategy to achieve more than 90% transduction efficiency was documented using recombinant adeno‐associated virus serotype 6 (rAAV6) vectors. However, the mechanisms of enhanced vector transduction efficiency in hematopoietic cells are largely unknown. In this manuscript, we first reported that proteasome inhibitors, which are well‐known to facilitate rAAV intracellular trafficking in various cell types, are not effective in hematopoietic cells. From the screening of small molecules derived from traditional Chinese medicine, we demonstrated that shikonin, a potential reactive oxygen species (ROS) generator, significantly increased the in vitro and ex vivo transgene expression mediated by rAAV6 vectors in hematopoietic cells, including human cord blood‐derived CD34 + HSPCs. Shikonin mainly targeted vector intracellular trafficking, instead of host cell entry or endonuclear single to double strand vector DNA transition, in a vector serotype‐dependent manner. Moreover, a ROS scavenger completely prevented the capability of shikonin to enhance rAAV6 vector‐mediated transgene expression. Taken together, these studies expand our understanding of rAAV6‐mediated transduction in hematopoietic cells and are informative for improving rAAV6‐based treatment of blood diseases.

show abstract

Section: Discussionmentioning

confidence: 88%

mentioning

confidence: 93%

Mechanism for enhanced transduction of hematopoietic cells by recombinant adeno‐associated virus serotype 6 vectors

Chen

Jia

et al. 2020

FASEB j.

View full text Add to dashboard Cite

show abstract

“…It is also the source of a natural red dye that can be used as a coloring agent in food, beverage, and cosmetics [15]. A decoction made from its heartwood is used in Namya-Uthai as a cardiotonic and to quench thirst [16]. In Northern Thailand, especially in the Chiang Mai, Nan, and Lampang provinces, a CS heartwood decoction is used as an anti-inflammatory agent for the treatment of traumatic disease and arthritis [13].…”

Section: Introductionmentioning

confidence: 99%

“…In Northern Thailand, especially in the Chiang Mai, Nan, and Lampang provinces, a CS heartwood decoction is used as an anti-inflammatory agent for the treatment of traumatic disease and arthritis [13]. Many biological properties of CS have been reported such as antioxidant [12,13,17,18], antibacterial [15,16,19], anti-inflammatory [17,20], hypoglycemic [21,22], and hepatoprotective [23] activities. In addition, the constituents of CS extract exhibited high activity against the influenza virus (H3N2) [24].…”

Section: Introductionmentioning

confidence: 99%

Effect of Ethanolic Caesalpinia sappan Fraction on In Vitro Antiviral Activity against Porcine Reproductive and Respiratory Syndrome Virus

Arjin

Hongsibsong

Pringproa

et al. 2021

Veterinary Sciences

View full text Add to dashboard Cite

Porcine reproductive and respiratory syndrome virus (PRRSV) is a major epidemic in pig production, leading to economic losses in the pig industry worldwide. The use of medicinal plants with antiviral properties might be useful help to prevent and control PRRSV outbreaks. Caesalpinia sappan (CS) heartwood is an important herbal ingredient used in Thai folk medicine, possessing various biological activities, including antiviral activity. The present study focuses on the in vitro antiviral activity against PRRSV of a semi-purified fraction of ethanolic CS crude extract using preparative high-performance liquid chromatography. Qualification of the fractions illustrating positive antiviral activity was carried out with liquid chromatography–quadrupole time-of-flight mass spectrometry. The preparative chromatography separated the crude extract into six consecutive fractions, among which the first fraction showed potential antiviral activity by inhibiting PRRSV replication in a MARC-145 monolayer (virus titer 2.75 median tissue culture infective dose (TCID50)/mL (log10) vs. 9.50 median log10 TCID50/mL of the control) at 72 h post-infection, and this fraction included byakangelicin, brazilin, naringenin, and brazilein. These results provide useful information for further study to effectively develop the CS bioactive antiviral compounds against PRRSV as a feed additive or veterinary drug in the pig industry.

show abstract

“…Dried heartwood of sappan has long been used as herbal medicines as it contains many phytochemicals and bioactive compounds 1 – 5 . The biological abilities of the wood extract in terms of antiacne 6 , anti-bacterial 7 , 8 , anticancer 9 , antidiabetic 10 , antidiarrhea 11 , antiinflammatory 12 – 14 , antioxidant 15 and anti-photoaging 16 activities have been demonstrated by a number of researchers.…”

Section: Introductionmentioning

confidence: 99%

Color and molecular structure alterations of brazilein extracted from Caesalpinia sappan L. under different pH and heating conditions

Ngamwonglumlert

Devahastin

Chiewchan

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Brazilein extract from sappan wood (Caesalpinia sappan L.) has potential for use as natural food colorant since it has no unique flavor and taste. Although brazilein has long been applied in several traditional foods and beverages, information on its stability, which is of importance for practical application, is still limited. In this work, brazilein was isolated from sappan wood; its purity was confirmed by nuclear magnetic resonance spectroscopy. Relations between molecular structures and color as well as thermal stabilities of brazilein in aqueous solutions at pH 3, 7 and 9 were for the first time investigated. At the lowest pH, zero net-charge structure of brazilein, which exhibited yellow color, was predominantly found. The deprotonated and fully deprotonated structures of brazilein, which exhibited orange and red colors, respectively, were found when pH of the aqueous solutions increased. The forms of brazilein existing at the higher pH suffered extensive degradation upon heating, while the form existing at the lowest pH possessed higher stability. Heat-induced deprotonation and degradation were confirmed by UV-visible and Fourier-transform infrared spectra as well as losses of brazilein content. Sappan (Caesalpinia sappan L.), which has several such local names as Buckham wood in English, Bois de sappan in French, Pau de sappan in Polish, Sappanholz in German, Su mu in Chinese, Suo in Japanese and Fang in Thai, is a wood in the family Leguminosae. This wood is naturally available throughout Asia, including China, Japan and Thailand. Due to its several valuable functions, this wood plant is also nowadays cultivated in various other regions of the world, including Africa, Europe as well as North and South Americas. Dried heartwood of sappan has long been used as herbal medicines as it contains many phytochemicals and bioactive compounds 1-5. The biological abilities of the wood extract in terms of antiacne 6 , anti-bacterial 7,8 , anticancer 9 , antidiabetic 10 , antidiarrhea 11 , antiinflammatory 12-14 , antioxidant 15 and anti-photoaging 16 activities have been demonstrated by a number of researchers. The major active ingredient of sappan is brazilin, which is a colorless phenolic compound consisting of two aromatic rings, one pyrone and one five-membered ring. Based on its molecular structure, brazilin is identified as a neoflavonoid. However, hydroxyl group in the brazilin structure is easily oxidized and can change into carbonyl group, resulting in the structural transformation and formation of brazilein, which is a colored compound 17-19. Brazilein has indeed widely been used as a natural colorant; sappan extract containing brazilein has traditionally been used in the painting of books as well as in the dyeing of silk and wool. Since an aqueous extract of sappan possesses many beneficial properties over other red-color pigments, e.g.

show abstract

Optimization of Caesalpinia sappan L. heartwood extraction procedure to obtain the highest content of brazilin and greatest antibacterial activity

Cited by 29 publications

References 24 publications

Mechanism for enhanced transduction of hematopoietic cells by recombinant adeno‐associated virus serotype 6 vectors

Mechanism for enhanced transduction of hematopoietic cells by recombinant adeno‐associated virus serotype 6 vectors

Effect of Ethanolic Caesalpinia sappan Fraction on In Vitro Antiviral Activity against Porcine Reproductive and Respiratory Syndrome Virus

Color and molecular structure alterations of brazilein extracted from Caesalpinia sappan L. under different pH and heating conditions

Contact Info

Product

Resources

About