Optimization of capillary electrophoresis method with contactless conductivity detection for the analysis of tobramycin and its related substances

El-Attug, Mohamed; Hoogmartens, Jos; Adams, Erwin; Schepdael, Ann Van

doi:10.1016/j.jpba.2011.09.032

Cited by 40 publications

(19 citation statements)

References 27 publications

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“…The CE–C 4 D technique has been applied increasingly for the determination of many pharmaceutical and biological entities . Specifically, the CE–C 4 D method was used for the determination of many antibiotics, for example, colistin and tobramycin . The analytical method development with C 4 D detection for the determination of the three selected antibiotics has not been reported so far.…”

Section: Introductionmentioning

confidence: 99%

Capillary electrophoresis with capacitively coupled contactless conductivity detection method development and validation for the determination of azithromycin, clarithromycin, and clindamycin

Paul

Duchateau

Griend

et al. 2017

J of Separation Science

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A capillary electrophoresis with capacitively coupled contactless conductivity detection based method for the assay of azithromycin, clarithromycin and clindamycin was optimized and validated in this study. A buffer solution of 20 mM 2-(N-morpholino) ethane sulfonic acid, 40 mM l-histidine and 0.6 mM cetyltrimethylammonium bromide (pH 6.39) was used for the electrophoresis. An uncoated, bare-fused silica capillary (total length 60 cm, effective length 32 cm, 75 μm id) was used at 25°C. The sample was injected hydrodynamically at 0.5 psi for 5 s. The electrophoresis was conducted at 30 kV in reverse polarity for 6 min with 3 and 2 min of in-between sodium hydroxide (0.1 M) and background electrolyte rinsing, respectively. Ammonium acetate was used as internal standard. This simple and robust method showed reasonable limit of detection and limit of quantitation for azithromycin (0.0125/0.03 mg/mL), clarithromycin (0.017/0.03 mg/mL), and clindamycin (0.038/0.06 mg/mL), with good selectivity, precision both intraday (relative standard deviation ≤ 1.0%) and interday (relative standard deviation < 3.7%), linearity (R > 0.999) and recovery (99 - 101.7%). The method was successfully applied for the determination of azithromycin, clarithromycin and clindamycin in formulations.

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Section: Introductionmentioning

confidence: 99%

Capillary electrophoresis with capacitively coupled contactless conductivity detection method development and validation for the determination of azithromycin, clarithromycin, and clindamycin

Paul

Duchateau

Griend

et al. 2017

J of Separation Science

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“…These methods were applied for the detection of drug residues in a wide range of biological derived matrices such as cell tissues [15], serum [16], milk [17], eggs [15,18], and honey [18]. Chemical methodologies included the application of gas chromatography (GC) [19], thin layer chromatography (TLC) [20], high-performance liquid chromatography (HPLC) [21] and capillary electrophoresis (CE) [22]. Biological methodologies included the development of microbiological assays [23], radiochemical and radioimmunochemical assays (RIA) [24], enzyme linked- and fluoro- immunoassays (ELISA, FIA) [16, 25, 26], nano-sensors and nano-particle based immunoassays [27,28].…”

Section: Introductionmentioning

confidence: 99%

Development of generic immunoassay for the detection of a series of aminoglycosides with 6′-OH group for the treatment of genetic diseases in biological samples

Shalev

Kandasamy

Skalka

et al. 2013

Journal of Pharmaceutical and Biomedical Analysis

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Over the last two decades, a growing number of scientific evidences highlighted the potential therapeutic value of several structures of aminoglycoside antibiotics (including gentamicin and G418) for the treatment of various genetic diseases caused by nonsense mutations. These findings resulted in a fast evolvement of synthetic derivatives of aminoglycosides which were shown to be more target specific and less toxic than the clinically used antibiotics. The emerging progress in drug design and development has necessitated the urge to develop a fast, easy and accurate procedure for the determination of these potential therapeutic agents in various biologically derived matrices. Here we describe the preparation of a generic polyclonal antibody that was used for the development of homologous and heterologous immunoassays for the detection of a wide range of natural and synthetic aminoglycoside derivatives, highlighted today as potential therapeutic agents for the treatment of various genetic diseases. A common two-ring scaffold, NB82, present in the majority of compounds exhibiting potent biological activity, was used as a generic immunization hapten for the immunization of two rabbits. By using a series of chemical steps, NB82 was selectively conjugated via the N-1 position through glutaric acid linker to a carrier protein. Sensitivity (I50) values for the recognition of three representative compounds NB82, NB84 and NB124 were determined to be 10±3 ng mL−1, 0.5±0.04 μg mL−1 and 1±0.12 μg mL−1, respectively. Limits of detection were determined to be 1±0.3 ng mL−1 for NB82, 20±7 ng mL−1 for NB84 and 15±8 ng mL−1 for NB124. The developed assays were further exploited for the in-vivo monitoring of the therapeutic compounds in mice serum. Serum experimentations exhibited similar detection limits as observed for the PBS calibration experiments, demonstrating no interference with assays sensitivity, with rather high recovery ratios ranging from 92–107% in whole blood samples.

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“…Several analytical techniques were reported for the analysis of TOB in dosage forms and in biological fluids including spectrophotometry,6–9 spectrofluorimetry,6,7,10 capillary electrophoresis,11 and TLC densitometry 12,13. A number of high- performance liquid chromatography (HPLC) methods were described using specific detection modes such as evaporative light scattering detection,14,15 pulsed electrochemical detector, and tandem mass spectrometry 16–18.…”

Section: Introductionmentioning

confidence: 99%

Utility of Experimental Design in Pre-Column Derivatization for the Analysis of Tobramycin by HPLC—Fluorescence Detection: Application to Ophthalmic Solution and Human Plasma

El‐Zaher

Mahrouse

2013

Anal Chem�Insights

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A novel, selective, and sensitive reversed phase high-performance liquid chromatography (HPLC) method coupled with fluorescence detection has been developed for the determination of tobramycin (TOB) in pure form, in ophthalmic solution and in spiked human plasma. Since TOB lacks UV absorbing chromophores and native fluorescence, pre-column derivatization of TOB was carried out using fluorescamine reagent (0.01%, 1.5 mL) and borate buffer (pH 8.5, 2 mL). Experimental design was applied for optimization of the derivatization step. The resulting highly fluorescent stable derivative was chromatographed on C18 column and eluted using methanol:water (60:40, v/v) at a flow rate of 1 mL min−1. A fluorescence detector (λex 390 and λem 480 nm) was used. The method was linear over the concentration range 20–200 ng mL−1. The structure of the fluorescent product was proposed, the method was then validated and applied for the determination of TOB in human plasma. The results were statistically compared with the reference method, revealing no significant difference.

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Optimization of capillary electrophoresis method with contactless conductivity detection for the analysis of tobramycin and its related substances

Cited by 40 publications

References 27 publications

Capillary electrophoresis with capacitively coupled contactless conductivity detection method development and validation for the determination of azithromycin, clarithromycin, and clindamycin

Capillary electrophoresis with capacitively coupled contactless conductivity detection method development and validation for the determination of azithromycin, clarithromycin, and clindamycin

Development of generic immunoassay for the detection of a series of aminoglycosides with 6′-OH group for the treatment of genetic diseases in biological samples

Utility of Experimental Design in Pre-Column Derivatization for the Analysis of Tobramycin by HPLC—Fluorescence Detection: Application to Ophthalmic Solution and Human Plasma

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