Optimization of cardiac cine in the rat on a clinical 1.5-T MR system

Montet-Abou, Karin; Daire, Jean-Luc; Ivancevic, Marko K.; Hyacinthe, Jean-Noël; Nguyen, Duy; Jorge-Costa, Manuel; Morel, Denis R.; Vallée, Jean‐Paul

doi:10.1007/s10334-006-0037-z

Cited by 14 publications

(9 citation statements)

References 26 publications

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“…In addition, quantitative assessment of cardiac volumes, mass and infarct size provided robust results with high inter-study, inter-reader and intra-reader reproducibility. The high reproducibility for the quantification of left-ventricular volumes and mass was in accordance to previously published data by Montet-Abou et al [17] who investigated nine normal rats with four standard MR fast gradient- echo sequences using a 1.5 Tesla MR system. However, inter-, and intra-reader reproducibility was limited to four rats.…”

Section: Discussionsupporting

confidence: 89%

Reproducibility of small animal cine and scar cardiac magnetic resonance imaging using a clinical 3.0 tesla system

et al. 2013

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BackgroundTo evaluate the inter-study, inter-reader and intra-reader reproducibility of cardiac cine and scar imaging in rats using a clinical 3.0 Tesla magnetic resonance (MR) system.MethodsThirty-three adult rats (Sprague–Dawley) were imaged 24 hours after surgical occlusion of the left anterior descending coronary artery using a 3.0 Tesla clinical MR scanner (Philips Healthcare, Best, The Netherlands) equipped with a dedicated 70 mm solenoid receive-only coil. Left-ventricular (LV) volumes, mass, ejection fraction and amount of myocardial scar tissue were measured. Intra-and inter-observer reproducibility was assessed in all animals. In addition, repeat MR exams were performed in 6 randomly chosen rats within 24 hours to assess inter-study reproducibility.ResultsThe MR imaging protocol was successfully completed in 32 (97%) animals. Bland-Altman analysis demonstrated high intra-reader reproducibility (mean bias%: LV end-diastolic volume (LVEDV), -1.7%; LV end-systolic volume (LVESV), -2.2%; LV ejection fraction (LVEF), 1.0%; LV mass, -2.7%; and scar mass, -1.2%) and high inter-reader reproducibility (mean bias%: LVEDV, 3.3%; LVESV, 6.2%; LVEF, -4.8%; LV mass, -1.9%; and scar mass, -1.8%). In addition, a high inter-study reproducibility was found (mean bias%: LVEDV, 0.1%; LVESV, -1.8%; LVEF, 1.0%; LV mass, -4.6%; and scar mass, -6.2%).ConclusionsCardiac MR imaging of rats yielded highly reproducible measurements of cardiac volumes/function and myocardial infarct size on a clinical 3.0 Tesla MR scanner system. Consequently, more widely available high field clinical MR scanners can be employed for small animal imaging of the heart e.g. when aiming at serial assessments during therapeutic intervention studies.

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Section: Discussionsupporting

confidence: 89%

Reproducibility of small animal cine and scar cardiac magnetic resonance imaging using a clinical 3.0 tesla system

et al. 2013

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“…We obtain an ejection fraction of 81% in the normal rat, that is in agreement with values obtained in the literature using cardiac gated MRI [9,32,34,35]. Using hfMRI or cMRI, 2D slices of ten to 26 phases per cardiac cycle are acquired in TAT of 2 min per slice (Table 2) [2,9,31,34]. Even if the 3D isotropic acquisition takes a longer TAT, it allows to make a direct measurement of the left ventricular volume rather than an evaluation based on a geometric ellipsoidal model.…”

Section: Biological Applications Of Low Field Mrisupporting

confidence: 90%

“…Nowadays, two kinds of MRI systems are used for rat imaging: dedicated high magnetic field magnets (hfMRI) [2][3][4][5][6][7] rather present in specialized imaging centres for small animal imaging and clinical MRI (cMRI) devices widely spread but with accessibility for small animal imaging limited in time [8][9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Low field magnetic resonance imaging in rat in vivo

Breton¹,

Goetz²,

Choquet³

et al. 2008

IRBM

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“…The thoracotomy only group had an LV ejection fraction of 67.56 ± 2.86%, which falls within the range of expected ejection fractions for normal rat hearts [23]. The hearts that underwent ligation of the LAD to induce a myocardial infarction followed by injection of VEGF-transfected myoblasts showed no significant decrease in LV ejection fraction (61.68 ± 2.83%) compared to the control, i.e.…”

Section: Resultssupporting

confidence: 53%

Bioreducible polymer-transfected skeletal myoblasts for VEGF delivery to acutely ischemic myocardium

McGinn

Nam

et al. 2011

Biomaterials

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Implantation of skeletal myoblasts to the heart has been investigated as a means to regenerate and protect the myocardium from damage after myocardial infarction. While several animal studies utilizing skeletal myoblasts have reported positive findings, results from clinical studies have been mixed. In this study we utilize a newly developed bioreducible polymer system to transfect skeletal myoblasts with a plasmid encoding vascular endothelial growth factor (VEGF) prior to implantation into acutely ischemic myocardium. VEGF has been demonstrated to promote revascularization of the myocardium following myocardial infarction. We report that implanting VEGF expressing skeletal myoblasts into acutely ischemic myocardium produces superior results compared to implantation of untransfected skeletal myoblasts. Skeletal myoblasts expressing secreted VEGF were able to restore cardiac function to non-diseased levels as measured by ejection fraction, to limit remodeling of the heart chamber as measured by end systolic and diastolic volumes, and to prevent myocardial wall thinning. Additionally, arteriole and capillary formation, retention of viable cardiomyocytes, and prevention of apoptosis was significantly improved by VEGF expressing skeletal myoblasts compared to untransfected myoblasts. This work demonstrates the feasibility of using bioreducible cationic polymers to create engineered skeletal myoblasts to treat acutely ischemic myocardium.

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Optimization of cardiac cine in the rat on a clinical 1.5-T MR system

Abstract: Using optimized sequences on a clinical 1.5-T MR scanner can provide accurate quantification of cardiac function in small animals and can promote cardiovascular research on small animals at 1.5-T.

Cited by 14 publications

References 26 publications

Reproducibility of small animal cine and scar cardiac magnetic resonance imaging using a clinical 3.0 tesla system

Reproducibility of small animal cine and scar cardiac magnetic resonance imaging using a clinical 3.0 tesla system

Low field magnetic resonance imaging in rat in vivo

Bioreducible polymer-transfected skeletal myoblasts for VEGF delivery to acutely ischemic myocardium

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