Optimization of carvacrol, rosmarinic, oleanolic and ursolic acid extraction from oregano herbs (Origanum onites L., Origanum vulgare spp. hirtum and Origanum vulgare L.)

Abstract

The aim of our study was to increase the extraction efficiency of carvacrol, rosmarinic, oleanolic and ursolic acid from the different species of oregano herbs (Origanum onites L., Origanum vulgare spp. hirtum and Origanum vulgare L.). Various extraction methods (ultrasound-assisted, heat-reflux, continuous stirring, maceration, percolation) and extraction conditions (different solvent, material:solvent ratio, extraction temperature, extraction time) were used, and the active substances were determined by HPLC. The lowest content of carvacrol, rosmarinic, oleanolic and ursolic acid was obtained by percolation. During heat-reflux extraction, the content of active substances depended on the solvent used: ethanol/non-aqueous solvent (glycerol or propylene glycol) mixture was more effective compared with ethanol alone. The results showed that for each species of oregano the most optimal extraction method should be selected to maximize the content of biologically active substances in the extracts.

Repellency of the Origanum onites L. essential oil and constituents to the lone star tick and yellow fever mosquito

“…As the carvacrol content is high (75.70%) in our sample (Table S1), the current O. onites sample is considered as carvacrol-type. In the recent study, Baranauskaitė et al (2016) reported that carvacrol content found depended on the oregano species and the extraction methods.…”

The Influence of Different Oregano Species on the Antioxidant Activity Determined Using HPLC Postcolumn DPPH Method and Anticancer Activity of Carvacrol and Rosmarinic Acid

“…Prepared extract was filtered using vacuum filter. These conditions were determined in our previous study as the best for the extraction of main active compounds of Turkish Oregano [ 18 ].…”

“…The linear calibration curve was made and expressed by the following quadratic equation: R 2 (RA) = 0.999918, ( y = 2.01 · 10 7 x + 5.52 · 10 3 ), the linearity range: 10–100 μ g/ml. ( Figure 1(a) ) [ 18 ]. For determination of CA: the mobile phase was composed of methanol and water (60/40, v/v).…”

“…The quantification has been carried out by the external standard method. The calibration curve was made and expressed by the following quadratic equation: R 2 (CA) = 0.999751, ( y = 4.99 ∗ 10 9 x + 1.08 ∗ 10 3 ), the linearity range: 0.18–3 μ g/ml ( Figure 1(b) ) [ 18 ].…”

Subcritical Water Extraction of Ursolic Acid from Hedyotis diffusa

“…Many extraction methods, all conventional, such as Soxhlet extraction [13], maceration [14], reflux extraction [15] and modern, e.g., ultrasonic-assisted extraction (UAE) [16], accelerated solvent extraction (ASE) and microwave-assisted extraction (MAE) [9] have been used to extract triterpenes and their derivatives from plant material. However, conventional organic solvent extractions have an energy-consuming nature, low extraction selectivity, lengthy extraction procedures, and cause environmental problems.…”