Optimization of cellulase and xylanase production by Aspergillus niger CECT 2700 using brewery spent grain based on Taguchi design

Morán-Aguilar, María Guadalupe; Calderón‐Santoyo, Montserrat; Domínguez, José Manuel; Aguilar‐Uscanga, María Guadalupe

doi:10.1007/s13399-021-01808-z

Cited by 12 publications

(3 citation statements)

References 34 publications

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“…The short 5-day incubation period can provide advantages such as lowering the risk of contamination and reducing costs associated with enzyme production [43]. Statistical analysis (orthogonal Taguchi design) performed by Morán-Aguilar and co-workers, indicated that the factor that most contributes to the expression of xylanases is fermentation time [67]. Prolonged incubation times lead to a sharp decrease in enzymatic activity, mainly due to the production of non-specific proteases secreted by the fungus, which could lead to the denaturation of synthesized xylanase.…”

Section: Discussionmentioning

confidence: 99%

Rice Husk, Brewer’s Spent Grain, and Vine Shoot Trimmings as Raw Materials for Sustainable Enzyme Production

Guimarães,

Mota,

Pereira

et al. 2024

Materials

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Solid by-products with lignocellulosic structures are considered appropriate substrates for solid-state fermentation (SSF) to produce enzymes with diverse industrial applications. In this work, brewer’s spent grain (BSG), rice husk (RH), and vine shoot trimmings (VSTs) were employed as substrates in SSF with Aspergillus niger CECT 2088 to produce cellulases, xylanases, and amylases. The addition of 2% (NH4)2SO4 and 1% K2HPO4 to by-products had a positive effect on enzyme production. Substrate particle size influenced enzyme activity and the overall highest activities were achieved at the largest particle size (10 mm) of BSG and RH and a size of 4 mm for VSTs. Optimal substrate composition was predicted using a simplex centroid mixture design. The highest activities were obtained using 100% BSG for β-glucosidase (363 U/g) and endo-1,4-β-glucanase (189 U/g), 87% BSG and 13% RH for xylanase (627 U/g), and 72% BSG and 28% RH for amylase (263 U/g). Besides the optimal values found, mixtures of BSG with RH or VSTs proved to be alternative substrates to BSG alone. These findings demonstrate that SSF bioprocessing of BSG individually or in mixtures with RH and VSTs is an efficient and sustainable strategy to produce enzymes of significant industrial interest within the circular economy guidelines.

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Section: Discussionmentioning

confidence: 99%

Rice Husk, Brewer’s Spent Grain, and Vine Shoot Trimmings as Raw Materials for Sustainable Enzyme Production

Guimarães,

Mota,

Pereira

et al. 2024

Materials

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“…central composite design (CCD) and Box-Behnken design (BBD). Recognized as a simple statistical tool, Taguchi technique has been widely applied in multiple studies [21][22][23] for the enhancement on protocol design and process efficacy.…”

Section: Introductionmentioning

confidence: 99%

Optimization of Endoglucanase Synthesis by Trichoderma harzianum via Taguchi Approach

Teo¹,

Wahab

2023

J.Trop.Life.Science

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The oil palm biomass produced by plantations and mills in Malaysia is the largest contributor to the nation’s agro-waste, with the oil palm leaves (OPL) topping the list. Nevertheless, the surplus of OPL might have applicability as the substrate for cultivating lignocellulolytic bio-degraders. Ipso facto, this study employed raw OPL as the carbon source for cultivating Trichoderma harzianum under solid-state fermentation (SSF). Optimization of the SSF process using the Taguchi orthogonal design to produce endoglucanase (CMCase) successfully established the optimal fermentation conditions as the following: 7.00 × 108 spore/g inoculum size, 50% moisture content, pH 12 Mandel’s medium, with 3-day incubation at 40°C. The crude enzyme cocktail exhibited the corresponding maximum activity of 417.49 U/g CMCase. The enzymatic activities were significantly affected by factors, moisture content, inoculum size, and initial pH (p-value < 0.05). In short, the high extracellular CMCase activity of the T. harzianum crude enzyme cocktail may prove valuable in accelerating the saccharification of cellulose for biofuel-and nanocellulose production.

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“…At the same time, the annual sugarcane production is about 1.6 billion tons, being therefore the main by-product of the sugar cane industry [8]. Both BSG and SCB have a high added value, Energies 2022, 15, 4101 2 of 12 being available for conversion to other valuable products, e.g., fermentable sugars, which are the carbon source for the growth of microorganisms and enzymes production [9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Development of Sustainable Biorefinery Processes Applying Deep Eutectic Solvents to Agrofood Wastes

et al. 2022

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The growing demand for renewable energies and the application of sustainable and economically viable biorefinery processes have increased the study and application of lignocellulosic biomass. However, due to lignocellulosic biomass recalcitrance hindering its efficient utilization, the pretreatment in the biorefinery is an essential stage for success in the process. Therefore, Deep Eutectic Solvent (DES) has emerged as a promising green pretreatment. During this study, the effect of choline chloride [ChCl]:glycerol and [ChCl]:urea on sugarcane bagasse and brewery bagasse is evaluated. Results have demonstrated that using [ChCl]:glycerol in SCB reduced about 80% and 15% for acid-soluble lignin and Klason lignin, respectively, and improved efficiency on saccharification yields, achieving conversions of 60, 80, and 100% for glucan, xylan, and arabinan, correspondingly. In the case of BSG saccharification yields, about 65% and 98% are attained for glucan and xylan, respectively, when [ChCl]:glycerol was employed. These results confirm the effectiveness and facility of DES pretreatment as a suitable method that can improve the biorefinery processes.

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Optimization of cellulase and xylanase production by Aspergillus niger CECT 2700 using brewery spent grain based on Taguchi design

Cited by 12 publications

References 34 publications

Rice Husk, Brewer’s Spent Grain, and Vine Shoot Trimmings as Raw Materials for Sustainable Enzyme Production

Rice Husk, Brewer’s Spent Grain, and Vine Shoot Trimmings as Raw Materials for Sustainable Enzyme Production

Optimization of Endoglucanase Synthesis by Trichoderma harzianum via Taguchi Approach

Development of Sustainable Biorefinery Processes Applying Deep Eutectic Solvents to Agrofood Wastes

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