OPTIMIZATION OF CELLULASE ENZYME PRODUCTION FROM Aspergillus oryzae FOR INDUSTRIAL APPLICATIONS

Abstract

Cellulases are the hydrolytic group of enzymes, responsible for release of sugars in the bioconversion of the cellulosic biomass into a variety of value added industrial products. Fungal isolated cellulases are well studied and playing a significant role in various industrial processes. Enzymatic depolymerisation of cellulosic material has been done by the various fungal isolated enzymes. In the present study, the cultivation conditions for cellulase production from Aspergillus species were optimized. Optimization of scarification conditions such as time course, inoculum size, carbon source and concentration, nitrogen source, various pH levels were performed for the production of extracellular carboxymethyl cellulase and endoglucanase enzyme. The result exhibited, 15 % inoculums size, corncobs 2 % concentration, Urea and medium pH 7 at 30oC supported high yield of carboxymethyl cellulase (38.80 U/ml/min) and exoglucanase enzyme (10.94 U/ml/min) through a submerged fermentation (SmF). In future biotechnological applications in cellulase enzyme production attain a vital role to obtain high degradable yield.

The effects of compound treatment of Aspergillus oryzae and fibrolytic enzyme on in vitro degradation, gas production and fermentative profile of maize silage and sugarcane silage

“…Aspergillus oryzae has a physical action on fibre due to the growth of mycelium and hyphae through the roughage, increasing bacterial access and colonization of the fibre fraction, increasing fibre digestibility (Giraldo et al ., 2008). Furthermore, fungi can produce a range of enzymes, fibrolytic and amylolytic, and phytohormones that stimulate the activities of microorganisms, which can also promote improvements in fibre digestibility (Sher et al ., 2017; Zayed, 2018). Similarly, the addition of fibrolytic enzyme may increase fibrolytic activity by stimulating the growth of ruminal microorganisms through the release of cell wall compounds (López-Aguirre et al ., 2016).…”

Gamma Rays Mediated Mutagenesis of Super Koji (Aspergillus oryzae) to enhance the Thermostability and Production of CMCases: Process Scale-up at Pilot Level

“…Due to easy optimization of temperature and pH and handling, submerged fermentation is used for cellulase and other enzymes production, while solid state fermentation is cost effective with high yield [22,23].…”