Optimization of cellulase production by a novel endophytic fungus Pestalotiopsis microspora TKBRR isolated from Thalakona forest

Goukanapalle, Praveen Kumar Reddy; Kumar, Kanderi Dileep; Rajoji, Girinath; Kumari, B. S. Shanthi; Bontha, Rajasekhar Reddy

doi:10.1007/s10570-020-03220-8

Cited by 29 publications

(9 citation statements)

References 40 publications

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“…Because of the various ecological relationships this genus has with plants, it has attracted attention for its cellulolytic abilities, with over 400 possible enzymes found through the study of a Pestalotiopsis isolated from a mangrove [ 63 ]. So far, there are some cellulolytic enzymes known from this genus that have been studied, such as the xylanase [ 64 ] or the cellulases [ 65 ]. Another single isolate obtained from the Political Constitution was Periconia CP1-A1C, which belongs to the dark septate fungi Pleosporales, with some isolates reported with thermostable β-Glucosidases suitable for biotechnological processes [ 66 ].…”

Section: Discussionmentioning

confidence: 99%

Fungi with history: Unveiling the mycobiota of historic documents of Costa Rica

Escudero-Leyva

Vieto²,

Avendaño³

et al. 2023

PLoS ONE

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We studied the physicochemical characteristics and mycobiota associated to five key historic documents from Costa Rica, including the Independence Act of Costa Rica from 1821. We used nondestructive techniques (i.e., ATR-FTIR and XRF) to determine paper and ink composition. Results show that some documents are composed of cotton-based paper, whereas others were made of wood cellulose with an increased lignin content. We also determined that the ink employed in some of the documents is ferrogallic. Cultivation and molecular techniques were used to characterize the fungi inhabiting the documents. In total, 22 fungal isolates were obtained: 15 from the wood-cellulose-based documents and seven from the other three cotton-based. We also tested the cellulolytic activity of the recovered fungi; 95% of the fungi presented cellulolytic activity correlated to their ability to cause deterioration of the paper. Results suggest that cotton-based paper is the most resistant to fungal colonization and that most of the isolates have cellulolytic activity. This work increases the knowledge of the fungal diversity that inhabits historic documents and its relationship with paper composition and provides valuable information to develop strategies to conserve and restore these invaluable documents.

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Section: Discussionmentioning

confidence: 99%

Fungi with history: Unveiling the mycobiota of historic documents of Costa Rica

Escudero-Leyva

Vieto²,

Avendaño³

et al. 2023

PLoS ONE

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“…Pestalotiopsis isolated from a mangrove (Arfi et al 2013). So far, there are some cellulolytic enzymes known from this genus that have been studied, such as the xylanase (Koh et al 2021) or the cellulases (Goukanapalle et al 2020). Another single isolate obtained from the Political Constitution was Periconia CP1-A1C, which belongs to the dark septate fungi Pleosporales, with some isolates reported with thermostable b-Glucosidases suitable for biotechnological processes (Harnpicharnchai et al 2009) (Borgi and Gargouri 2016).…”

Section: Discussionmentioning

confidence: 99%

“…So far, there are some cellulolytic enzymes known from this genus that have been studied, such as the xylanase (Koh et al . 2021) or the cellulases (Goukanapalle et al . 2020).…”

Section: Discussionmentioning

confidence: 99%

Fungi with history: Unveiling the mycobiota of historic documents of Costa Rica

Escudero-Leyva

Vieto²,

Avendaño³

et al. 2022

Preprint

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Through nondestructive techniques, we studied the physicochemical characteristics and mycobiota of five key historic documents from Costa Rica, including the Independence Act of Costa Rica from 1821. We determined that for documents dated between 1500 and 1900 (i.e., the Cloudy Days Act, the Independence Act, and two documents from the Guatemalan Series from 1539 and 1549), the paper composition was cotton, whereas the 1991 replicate of the Political Constitution from 1949 was made of wood cellulose with an increased lignin content. We also determined that the ink employed in 1821 documents is ferrogallic, i.e., formed by iron sulfate salts in combination with gallic and tannic acids. In total, 22 fungal isolates were obtained: 15 from the wood-cellulose-based Political Constitution and seven from the other three cotton-based documents. These results suggest that cotton-based paper is the most resistant to microbial colonization. Molecular identifications using three DNA markers (i.e., ITS nrDNA, beta-tubulin, and translation elongation factor 1-alpha) classified the isolates in eight orders and ten genera. The most frequent genera were Cladosporium, Penicillium, and Purpureocillium. Of the isolates, 95% presented cellulolytic activity correlated to their ability to cause deterioration of the paper. This work increases the knowledge of the fungal diversity that inhabits historic documents and its relationship with paper composition and provides valuable information to develop strategies to conserve and restore these invaluable documents.

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“…Trichoderma reesei [32] Cladosporium cladosporioides NS2 [33] Trichoderma reesei NCIM 1186 and Penicillium citrinum NCIM 768 [34] Aspergillus flavus [35] Penicillium sp., Cladosporium sp., Fusarium sp. [36] Trichoderma reesei RUT C30 [37] Talaromyces pinophilus EMU [38] Trichoderma reesei [39] Pestalotiopsis microspora TKBRR [40] Talaromyces emersonii [41] Trichoderma orientalis EU7-22 [42] Trichoderma reesei [43] Aspergillus niger [44] Trichoderma harzianum EM0925 [45] Humicola grisea var. thermoidea [46] Bacteria Bacillus pseudomycoides [47] Bacillus velezensis [48] Bacillus amyloliquefaciens FW2 [49] Lactobacillus plantarum RI 11 [50] Burkholderia sp.…”

Section: Fungimentioning

confidence: 99%

Integrating 1G with 2G Bioethanol Production by Using Distillers’ Dried Grains with Solubles (DDGS) as the Feedstock for Lignocellulolytic Enzyme Production

2022

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First-generation (1G) bioethanol is one of the most used liquid biofuels in the transport industry. It is generated by using sugar- or starch-based feedstocks, while second-generation (2G) bioethanol is generated by using lignocellulosic feedstocks. Distillers’ dried grains with solubles (DDGS) is a byproduct of first-generation bioethanol production with a current annual production of 22.6 million tons in the USA. DDGS is rich in fiber and valuable nutrients contents, which can be used to produce lignocellulolytic enzymes such as cellulases and hemicellulases for 2G bioethanol production. However, DDGS needs a pretreatment method such as dilute acid, ammonia soaking, or steam hydrolysis to release monosaccharides and short-length oligosaccharides as fermentable sugars for use in microbial media. These fermentable sugars can then induce microbial growth and enzyme production compared to only glucose or xylose in the media. In addition, selection of one or more suitable microbial strains, which work best with the DDGS for enzyme production, is also needed. Media optimization and fermentation process optimization strategies can then be applied to find the optimum conditions for the production of cellulases and hemicellulases needed for 2G bioethanol production. Therefore, in this review, a summary of all such techniques is compiled with a special focus on recent findings obtained in previous pieces of research conducted by the authors and by others in the literature. Furthermore, a comparison of such techniques applied to other feedstocks and process improvement strategies is also provided. Overall, dilute acid pretreatment is proven to be better than other pretreatment methods, and fermentation optimization strategies can enhance enzyme production by considerable folds with a suitable feedstock such as DDGS. Future studies can be further enhanced by the technoeconomic viability of DDGS as the on-site enzyme feedstock for the manufacture of second-generation bioethanol (2G) in first-generation (1G) ethanol plants, thus bridging the two processes for the efficient production of bioethanol using corn or other starch-based lignocellulosic plants.

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Optimization of cellulase production by a novel endophytic fungus Pestalotiopsis microspora TKBRR isolated from Thalakona forest

Cited by 29 publications

References 40 publications

Fungi with history: Unveiling the mycobiota of historic documents of Costa Rica

Fungi with history: Unveiling the mycobiota of historic documents of Costa Rica

Fungi with history: Unveiling the mycobiota of historic documents of Costa Rica

Integrating 1G with 2G Bioethanol Production by Using Distillers’ Dried Grains with Solubles (DDGS) as the Feedstock for Lignocellulolytic Enzyme Production

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