OPTIMIZATION OF CHLOROPHYLL EXTRACTION FROM Gynura procumbens

Abstract

Gynura procumbens (Sambung nyawa) is a bicolor (green and red) herbal plant, which is widely grown in Asia. This herbs are famously used for medicinal purposes as a safe alternative to chemical-based medicine. Recent studies proved that this plant has anti-herpes simplex virus, anti-flammatory and anti-hyperglycaemic properties. This work investigates the extraction of chlorophyll a and b in Gynura procumbens using Solid liquid extraction method. The solid to solvent ratio, temperature, solvent used and extraction time were varied to determine the optimum conditions for extraction. It was found that at 80 C in 90 minutes with 2:5 solid to solvent ratio was the most favorable condition to extract chlorophyll a and b. Five valuable compounds were found from GC-MS analysis, which are 2-hexanal, phenol, oleic acid, copaene and phytol. This implies that Gynura procumbens promises a good source of many useful bioactive compounds.Keywords: Gynura procumbens, sambung nyawa, chlorophyll, extraction, optimization Abstrak Sambung Nyawa ialah pokok herba dwi-warna yang tumbuh secara meluas di Asia. Herba ini sangat popular digunakan sebagai bahan alternatif yang selamat bagi tujuan perubatan berbanding ubatan berunsurkan bahan kimia. Penyelidikan terbaru membuktikan bawaha pokok ini anti-virus herpes simplex, anti-keradangan dan anti-sifat hiperglisemia. Eksperimen ini menyiasat tentang pengekstrakan klorofil a dan b dalam Gynura procumbens. Menggunakan pengekstrakan cecair pepejal. Nisbah cecair pepejal, suhu, pelarut dan masa mengekstrak telah dimanipulasi untuk menentukan keadaan yang optimum untuk proses pengekstrakan tersebut. Keputusan eksperimen mendapati bahawa pengekstrakan selama 90 minit pada suhu 80C serta nisbah cecair pepejal 2:5 merupakan kondisi yang paling sesuai untuk mengekstrak klorofil a dan b. Lima kompoun berharga telah ditemui melalui keputusan analisa GC-MS iaitu 2-heksanal, fenol, asid oleik, copaene and fitol. Keputusan ini mencadangkan potensi Gynura procumbens sebagai sumber yang baik kepada sebatian bioaktif.

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Gynura procumbens ethanol extract and its fractions inhibit Th1, Th2 and Th17 but induce Treg cells differentiation during atherosclerosis development

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