Optimization of chromatographic selectivity of twelve sulphonamides in reversed-phase high-performance liquid chromatography using mixture designs and multi-criteria decision making

Separation of sulfonamides by capillary electrochromatography

“…However, of these six compounds only SISM displayed significant electrophoretic behaviour. The rest of the compounds showed chromatographic behaviour and the observed elution pattern was similar to that reported by Wieling et al [28] (SGW<SISM<ST<SPRY< SMR < SAA < SCP < SMOZ) when they separated sulfonamides by reversed phase chromatography at pH 3.0. In the present work, SAA eluted soon after sulfaguanidine.…”

“…The elution order observed was similar to that obtained on a reversedphase chromatographic system [28]. At this pH SISM eluted as a double peak at 11.71 minutes, with part of it co-eluting with SGW at 11.31 minutes.…”

Buffered superheated water as an eluent for reversed-phase high performance liquid chromatography

“…They also examined the effect of changing the proportion of methanol but there was little effect from 10 to 16% for these analytes. Wieling and coworkers [7], also found a similar order of retention at pH 3.0, except that in both methanol and acetonitrile as the modifier, sulfanilamide was the most highly retained of this group of compounds.…”

“…Changes in the retention of a series of sulfonamides on a polystyrene-divinylbenzene (PS-DVB) column with the pH of the eluent are compared with separations in conventional eluents. These drugs have been of considerable analytical interest over many years and a large number of methods have been reported for their determination, including reversed-phase liquid chromatography with buffered eluents on both ODS silica [6][7][8][9][10] and PS-DVB columns [11][12][13] and more recently capillary electrophoresis [14][15][16]. Changes in retention over a limited pH range are used to determine if the apparent pKa of the analytes is changed significantly by the elevated temperature.…”