Optimization of cis-9-Heptadecenoic Acid Production from the Oleaginous Yeast Yarrowia lipolytica

Sahyouni, Wendy Al; Kantar, Sally El; Khelfa, Anissa; Park, Young Kyoung; Nicaud, Jean‐Marc; Louka, Nicolas; Koubàa, Mohamed

doi:10.3390/fermentation8060245

Cited by 8 publications

(5 citation statements)

References 42 publications

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“…However, the highest growth rate of JMY9398 did not correspond to the highest titer of 2-PE, which was 0.56 g/L of 2-PE in a medium composed of 40 g/L SBM and 5 g/L YE (Figure 3c,e). Increasing the concentration of YE would increase cell density rather than 2-PE production, since YE is a nitrogen source known to favor cellular proliferation [42].…”

Section: Optimization Of the Sbm-based Culture Media Compositionmentioning

confidence: 99%

Bioproduction of 2-Phenylethanol by Yarrowia lipolytica on Sugar Beet Molasses as a Low-Cost Substrate

Mitri,

Louka,

Rossignol

et al. 2024

Fermentation

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2-phenylethanol (2-PE) is a valuable aromatic alcohol with diverse applications in cosmetics, food, beverages, and pharmaceutical industries. Currently, 2-PE is produced either through chemical synthesis or by extraction from plant materials. However, both conventional production methods have their own limitations. Therefore, there is a need for more eco-friendly and cost-effective approaches to produce natural 2-PE. Biotechnological routes, particularly microbial fermentations, hold promise for natural 2-PE production, especially when using low-cost substrates. In this study, 2-PE was produced by de novo synthesis via the shikimate pathway, using the yeast Yarrowia lipolytica in a medium composed of sugar beet molasses (SBM) and yeast extract (YE) as carbon and nitrogen sources, respectively. A genetically engineered strain was generated, in which the SUC2 gene was transformed, expressing the invertase enzyme, enabling Y. lipolytica to efficiently utilize SBM as a cost-effective substrate. A central composite design allowed for the optimization of the concentrations of the carbon and nitrogen sources, resulting in approximately 0.71 g(2-PE)/L(culture medium). The results obtained highlight the potential of utilizing SBM as a low-cost substrate for 2-PE production, advancing biotechnological approaches in fragrance synthesis.

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Section: Optimization Of the Sbm-based Culture Media Compositionmentioning

confidence: 99%

Bioproduction of 2-Phenylethanol by Yarrowia lipolytica on Sugar Beet Molasses as a Low-Cost Substrate

Mitri,

Louka,

Rossignol

et al. 2024

Fermentation

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“…Moreover, most of the published studies use non-GMOs for such conversion. Only a few studies have tested GMOs with pure VFAs or their salt forms [ 96 , 100 , 101 ]. Many questions thus arise regarding the challenges associated with performing a simultaneous acidogenesis and VFA conversion and the limited use of GMOs for such transformations.…”

Section: Challenges In the Conversion Of Vfas Into Valuable Products:...mentioning

confidence: 99%

Integrated Biorefinery for a Next-Generation Methanization Process Focusing on Volatile Fatty Acid Valorization: A Critical Review

Koubaa

2024

Molecules

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This review addresses the critical issue of a rapidly increasing worldwide waste stream and the need for sustainable management. The paper proposes an integrated transformation toward a next-generation methanization process, which leads not only to treating waste but also to converting it into higher value compounds and greener energy. Although the current and commonly used anaerobic digestion process is useful for biogas production, it presents limitations of resource exploitation and some negative environmental impacts. Focusing on the acidogenic stage in waste stream processing, the paper discusses the recent strategies to enhance the recovery of volatile fatty acids (VFAs). These acids serve as precursors for synthesizing a variety of biochemicals and biofuels, offering higher value products than solely energy recovery and soil fertilizers. Additionally, the importance of recycling the fermentation residues back into the biorefinery process is highlighted. This recycling not only generates additional VFAs but also contributes to generating clean energy, thereby enhancing the overall sustainability and efficiency of the waste management system. Moreover, the review discusses the necessity to integrate life cycle assessment (LCA) and techno-economic analysis (TEA) to evaluate the environmental impacts, sustainability, and processing costs of the proposed biorefinery.

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“…Y. lipolytica, a GRAS oleaginous yeast, is a versatile production chassis whose potential in this application among myriad others is bolstered by the availability of a large panoply of genetic toolkits enabling its genetic manipulation. To date, Y. lipolytica has been successfully modified to synthesize a wide range of fatty acids [24,25] and flavonoids, among other molecules of interest, which it produces as secondary metabolites [26,27]. Another unique feature of this microorganism is its ability to utilize numerous carbon sources including dextrose and glycerol.…”

Section: Of 11mentioning

confidence: 99%

Purification of Natural Pigments Violacein and Deoxyviolacein Produced by Fermentation Using Yarrowia lipolytica

et al. 2023

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Violacein and deoxyviolacein are bis-indole pigments synthesized by a number of microorganisms. The present study describes the biosynthesis of a mixture of violacein and deoxyviolacein using a genetically modified Y. lipolytica strain as a production chassis, the subsequent extraction of the intracellular pigments, and ultimately their purification using column chromatography. The results show that the optimal separation between the pigments occurs using an ethyl acetate/cyclohexane mixture with different ratios, first 65:35 until both pigments were clearly visible and distinguishable, then 40:60 to create a noticeable separation between them and recover the deoxyviolacein, and finally 80:20, which allows the recovery of the violacein. The purified pigments were then analyzed by thin-layer chromatography and nuclear magnetic resonance.

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Optimization of cis-9-Heptadecenoic Acid Production from the Oleaginous Yeast Yarrowia lipolytica

Cited by 8 publications

References 42 publications

Bioproduction of 2-Phenylethanol by Yarrowia lipolytica on Sugar Beet Molasses as a Low-Cost Substrate

Bioproduction of 2-Phenylethanol by Yarrowia lipolytica on Sugar Beet Molasses as a Low-Cost Substrate

Integrated Biorefinery for a Next-Generation Methanization Process Focusing on Volatile Fatty Acid Valorization: A Critical Review

Purification of Natural Pigments Violacein and Deoxyviolacein Produced by Fermentation Using Yarrowia lipolytica

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