Optimization of cleaning and amino‐ silanization protocols for Si wafers to be used as platforms for biochip microarrays by surface analysis (XPS, ToF‐SIMS and NEXAFS spectroscopy)

Graf, Nora; Yeğen, Eda; Lippitz, Andreas; Treu, Dieter; Wirth, Thomas; Unger, Wolfgang

doi:10.1002/sia.2621

Cited by 17 publications

(10 citation statements)

References 6 publications

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“…In a paper published recently [30] we described a protocol for the preparation of aminosiloxane layers on Si wafers with 3-aminopropyltrimethoxysilane (ABCR GmbH & Co. KG, Germany, purity 96%) and p-aminophenyltrimethoxysilane (ABCR GmbH & Co. KG, Germany, purity 90%) to obtain APS 1 and APhS 2, which was also applied in this study. Briefly, Si wafers were cleaned with acetone and water and then treated with ''piranha solution" (conc.…”

Section: Aminosiloxane Samplesmentioning

confidence: 99%

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XPS and NEXAFS studies of aliphatic and aromatic amine species on functionalized surfaces

et al. 2009

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Section: Aminosiloxane Samplesmentioning

confidence: 99%

“…A purification procedure using NH 3 /H 2 O 2 was applied as described in Ref. [30] and silanization based on the protocol for sciCHIP AMINO glass slide manufacturing. Table 1 Relevant nitrogen species which are supposed to occur at those aminated surfaces investigated in this study.…”

Section: Aminosiloxane Samplesmentioning

confidence: 99%

XPS and NEXAFS studies of aliphatic and aromatic amine species on functionalized surfaces

et al. 2009

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“…On the basis of the labelling reaction depicted in Scheme 5, the amount fraction of carbon atoms containing amino groups [NH 2 ] C that are reactive towards 5 can be derived from the fluorine content according to Equation (1). [13,17,[77][78][79][80][81]…”

Section: X-ray Photoelectron Spectroscopymentioning

confidence: 99%

Fluorinated Boron‐Dipyrromethene (BODIPY) Dyes: Bright and Versatile Probes for Surface Analysis

et al. 2013

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A family of bright boron-dipyrromethene-type fluorophores with a high number of fluorine atoms (F-BODIPYs) has been developed and characterized by X-ray crystallography and optical spectroscopy. The introduction of 3,5-bis(trifluoromethyl)phenyl and pentafluorophenyl moieties significantly enhances the photostability of such dyes, yielding for instance photostable near-infrared (NIR) fluorophores that show emission maxima>750 nm, when the BODIPY’s π system is extended with two (dimethylamino)styryl and (dimethylamino)naphthastyryl moieties, or green-emitting BODIPYs with fluorescence quantum yields of unity. When equipped with a suitable group that selectively reacts for instance with amines, F-BODIPYs can be used as potent dual labels for the quantification of primary amino groups on surfaces by X-ray photoelectron spectroscopy (XPS) and fluorescence, two powerful yet complementary tools for the analysis of organic surface functional groups. The advantage of reactive F-BODIPYs is that they allow a fast and non-destructive mapping of the labelled supports with conventional fluorescence scanners and a subsequent quantification of selected areas of the same sample by the potentially traceable XPS technique. The performance is exemplarily shown here for the assessment of the amino group density on SiO2 supports, one of the most common reactive silica supports, in particular, for standard microarray applications.

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“…APS reacts with inorganic surfaces such as silica, silica gels, , glass, quartz, alumina, , zeolites, iron, titania, germanium, and mica , through its −Si(OH) 3 group, and it reacts with polymer matrices through its amino group, providing strong adhesion at the polymer–inorganic material interface. Many studies have presented protocols for obtaining reproducible APS layers with a specific thickness, roughness, reactivity, and stability . Indeed, the structure and properties of APS layers can vary, depending on the nature of the substrate, the solvent used, deposition time and temperature, amount of hydrolyzed reactive siloxane groups in the APS molecule, and curing conditions .…”

Section: Introductionmentioning

confidence: 99%

Hydrolytic Stability of 3-Aminopropylsilane Coupling Agent on Silica and Silicate Surfaces at Elevated Temperatures

Okhrimenko

Budi

Ceccato

et al. 2017

ACS Appl. Mater. Interfaces

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3-Aminopropylsilane (APS) coupling agent is widely used in industrial, biomaterial, and medical applications to improve adhesion of polymers to inorganic materials. However, during exposure to elevated humidity and temperature, the deposited APS layers can decompose, leading to reduction in coupling efficiency, thus decreasing the product quality and the mechanical strength of the polymer-inorganic material interface. Therefore, a better understanding of the chemical state and stability of APS on inorganic surfaces is needed. In this work, we investigated APS adhesion on silica wafers and compared its properties with those on complex silicate surfaces such as those used by industry (mineral fibers and fiber melt wafers). The APS was deposited from aqueous and organic (toluene) solutions and studied with surface sensitive techniques, including X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), streaming potential, contact angle, and spectroscopic ellipsometry. APS configuration on a model silica surface at a range of coverages was simulated using density functional theory (DFT). We also studied the stability of adsorbed APS during aging at high humidity and elevated temperature. Our results demonstrated that APS layer formation depends on the choice of solvent and substrate used for deposition. On silica surfaces in toluene, APS formed unstable multilayers, while from aqueous solutions, thinner and more stable APS layers were produced. The chemical composition and substrate roughness influence the amount of deposited APS. More APS was deposited and its layers were more stable on fiber melt than on silica wafers. The changes in the amount of adsorbed APS can be successfully monitored by streaming potential. These results will aid in improving industrial- and laboratory-scale APS deposition methods and increasing adhesion and stability, thus increasing the quality and effectiveness of materials where APS is used as a coupling agent.

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Optimization of cleaning and amino‐ silanization protocols for Si wafers to be used as platforms for biochip microarrays by surface analysis (XPS, ToF‐SIMS and NEXAFS spectroscopy)

Cited by 17 publications

References 6 publications

XPS and NEXAFS studies of aliphatic and aromatic amine species on functionalized surfaces

XPS and NEXAFS studies of aliphatic and aromatic amine species on functionalized surfaces

Fluorinated Boron‐Dipyrromethene (BODIPY) Dyes: Bright and Versatile Probes for Surface Analysis

Hydrolytic Stability of 3-Aminopropylsilane Coupling Agent on Silica and Silicate Surfaces at Elevated Temperatures

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