Optimization of commercially available Zika virus antibodies for use in a laboratory‐developed immunohistochemical assay

Bollweg, Brigid C.; Silva‐Flannery, Luciana; Spivey, Pamela; Hale, Gillian

doi:10.1002/cjp2.84

Cited by 19 publications

(15 citation statements)

References 23 publications

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“…Also, the capability of this strain to infect fetus passing placental barrier is unknown and might be lower than the capability of the Asian lineage to cause fetal infection. The labeling observed was consistent to previously described ZIKV IHC regarding location and shape 21 , the antibody utilized targets ZIKV NS1. NS1 detection in serum is a diagnostic marker of Flavivirus infection 22 , this protein is involved in immune evasion and pathogenicity, and the antibody used in this study presents no cross reactivity with other flaviviruses 21 .…”

Section: Discussionsupporting

confidence: 88%

“…The labeling observed was consistent to previously described ZIKV IHC regarding location and shape 21 , the antibody utilized targets ZIKV NS1. NS1 detection in serum is a diagnostic marker of Flavivirus infection 22 , this protein is involved in immune evasion and pathogenicity, and the antibody used in this study presents no cross reactivity with other flaviviruses 21 . NS5 fragment sequencing revealed 3 mutations of which, 2 were located in the MTase coding region and 1 was in the RdRP coding region.…”

Section: Discussionsupporting

confidence: 88%

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Detection of a novel African-lineage-like Zika virus naturally infecting free-living neotropical primates in Southern Brazil

Almeida

Ehlers

Demoliner

et al. 2019

Preprint

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Mosquito borne flaviviruses cause a series of important diseases in humans and animals. These viruses are maintained in cycles involving replication in mosquito and in vertebrate hosts. Most natural hosts are vertebrate animals living in sylvatic or peridomestic environments. Human contact with these environments may result in host shifts that lead to the establishment of urban transmission cycles. Zika virus is a Flavivirus that persists in nature in a transmission cycle involving non-human primates (NHP). Its recent emergence in Brazil has shed light upon the importance of surveying this agent in Brazilian sylvatic environments. Here we present histopathological and molecular evidence that free ranging howler monkeys (Alouatta guariba) in Southern Brazil are infected by ZIKV closely related to African lineage MR766. Nine NHP were nested RT-PCR positive for ZIKV RNA. Sequence analysis revealed 96 to 98% identity to ZIKV MR766 and 85% identity to ZIKV P6-740, the current epidemic strain. The affected howler monkeys presented discrete inflammatory infiltrates in several tissues and immunohistochemichal (IHC) labeling of viral antigen was observed in placenta. These findings point to the circulation of African lineage Zika virus in the Americas in non-human primates. And raises the possibility that ZIKV was introduced into the Americas on more than one occasion. 1

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Section: Discussionsupporting

confidence: 88%

Section: Discussionsupporting

confidence: 88%

Detection of a novel African-lineage-like Zika virus naturally infecting free-living neotropical primates in Southern Brazil

Almeida

Ehlers

Demoliner

et al. 2019

Preprint

View full text Add to dashboard Cite

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“…Paraffin-embedded tissue samples were processed for histopathology and stained with hematoxylin and eosin (HE). For immunohistochemistry (IHC), an adapted Streptavidin Alkaline Phosphatase (SAAP) assay [8,13,14,15,16,17,18] with anti-ZIKV polyclonal mouse antibody. The polyclonal anti-ZIKV antibody used in the IHC assay was prepared at the Instituto Evandro Chagas (IEC, Ananindeua, Brazil) in young Swiss mice using a ZIKV strain isolated in cell culture (C6/36 cells).…”

Section: Methodsmentioning

confidence: 99%

Zika Virus Epidemic in Brazil. II. Post-Mortem Analyses of Neonates with Microcephaly, Stillbirths, and Miscarriage

Azevedo

Araújo

Oliveira

et al. 2018

JCM

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Introduction: The recent Zika virus(ZIKV) epidemic in Brazil was characterized by a range of different clinical presentations, particularly microcephaly, Guillain-Barré syndrome, and death. In this context, we determined the causal relationship between fatal microcephaly cases and ZIKV infection. Methods: Twelve fatal cases of neonates, whose mothers were infected with ZIKV during pregnancy, were examined; cases included nine neonatal deaths due to microcephaly, one miscarriage, and two stillbirths. Tissue samples were obtained from all cases at necropsy and were submitted for virological investigation (RT-qPCR and virus isolation) and/or histopathology (hematoxylin and eosin staining) and immunohistochemical assay for the detection of ZIKV antigens. Results: ZIKV antigens and/or ZIKV RNA were detected in tissue samples of all 12 cases examined. ZIKV was recovered in one case. Results of the virological and immunohistochemical analyses, as well as the anatomic abnormalities and histopathologic changes observed at necropsy on the 12 fatal cases, are presented. Conclusions: Data from these 12 cases provide strong evidence of the causal relationship between ZIKV and congenital disease in fetuses of women who were infected with the virus during pregnancy.

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“…Tetramethylbenzidine (TMBZ) was purchased from Dojindo (Kumamoto, Japan). Anti-ZIKV NS1 protein antibody was purchased from Gene Tex Inc. (Irvine, CA, USA), which does not react with any other type of flavivirus group of the NS1 protein [20]. Zika virus recombinant NS1 antigen was purchased from the Native Antigen (Oxford, UK).…”

Section: Methodsmentioning

confidence: 99%

A localized surface plasmon resonance-amplified immunofluorescence biosensor for ultrasensitive and rapid detection of nonstructural protein 1 of Zika virus

et al. 2019

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Among the members of flaviviruses, the Zika virus (ZIKV) remains a potent infectious disease agent, with its associated pandemic prompting the World Health Organization (WHO) to declare it a global public health concern. Thus, rapid and accurate diagnosis of the ZIKV is needed. In this study, we report a new immunofluorescence biosensor for the detection of nonstructural protein 1 (NS1) of the ZIKV, which operates using the localized surface plasmon resonance (LSPR) signal from plasmonic gold nanoparticles (AuNPs) to amplify the fluorescence intensity signal of quantum dots (QDs) within an antigen-antibody detection process. The LSPR signal from the AuNPs was used to amplify the fluorescence intensity of the QDs. For ultrasensitive, rapid, and quantitative detection of NS1 of the ZIKV, four different thiol-capped AuNPs were investigated. Our biosensor could detect the ZIKV in a wide concentration range from 10–107 RNA copies/mL, and we found that the limit of detection (LOD) for the ZIKV followed the order Ab-L-cysteine-AuNPs (LOD = 8.2 copies/mL) > Ab-3-mercaptopropionic acid-AuNPs (LOD = 35.0 copies/mL). Immunofluorescence biosensor for NS1 exhibited excellent specificity against other negative control targets and could also detect the ZIKV in human serum.

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Optimization of commercially available Zika virus antibodies for use in a laboratory‐developed immunohistochemical assay

Cited by 19 publications

References 23 publications

Detection of a novel African-lineage-like Zika virus naturally infecting free-living neotropical primates in Southern Brazil

Detection of a novel African-lineage-like Zika virus naturally infecting free-living neotropical primates in Southern Brazil

Zika Virus Epidemic in Brazil. II. Post-Mortem Analyses of Neonates with Microcephaly, Stillbirths, and Miscarriage

A localized surface plasmon resonance-amplified immunofluorescence biosensor for ultrasensitive and rapid detection of nonstructural protein 1 of Zika virus

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