Optimization of condition extraction in quantification of total flavonoid content in the seeds of the Arummanis (Mangifera indica L.) mango from Indonesia

Masturi, Masturi; Alighiri, Dante; Nuzulina, Khoirun; Rodhiyah, M.; Drastisianti, Apriliana

doi:10.1088/1742-6596/1321/2/022041

Cited by 10 publications

(3 citation statements)

References 9 publications

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“…This is because ethanol is superior to water and methanol regarding its traceability. According to Masturi et al, [24], the optimal total flavonoid content of harumanis seeds was obtained using a 96% ethanol solvent. Thus, it can be concluded that organic solvents derived from water, such as 50% ethanol and 50% methanol, were notably effective at extracting the flavonoid content of harumanis leaves.…”

Section: Total Phenolic and Flavonoid Contents Of Harumanis Leaves Ex...mentioning

confidence: 99%

Insights into the Pharmacognostic Elucidation of Harumanis Mango (Mangifera Indica Linn.) Leaves Extracts as Therapeutic Agent

Ryan Vitthaya Peron,

Amirul Ridzuan Abu Bakar,

Mohd Asraf Mohd Zainuddin

et al. 2024

ARMNE

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Mangifera indica L. (mango) of the Anacardiaceae family is a fruit with many phytochemicals that are extensively used in various traditional systems of medicine to prevent and treat various diseases. In Malaysia, harumanis is a local variety of mango widely cultivated in the northern region, namely Perlis. However, the drawback of this harumanis variety of mango is that research and information on its therapeutics are scarce and limited. Therefore, localized harumanis leaves were introduced to a series of pharmacognostic studies to provide critical details explaining harumanis as a potential therapeutic agent. Initially, the harumanis leaves were introduced to quality analysis tests, unveiling that the raw material used abided by proper post-harvesting protocols. Then, the harumanis leaves were extracted using ultrasonic aided extraction (UAE) with 100% methanol (13.73±0.24 %), 50% methanol (14.47±0.19 %), 100% ethanol (10.35±0.19 %), 50% ethanol (12.61±0.26 %), and distilled water (8.09±0.21 %). Next, the initial phytochemical screening revealed the presence of flavonoids, tannins, and saponins in all extracts. Subsequently, the phenolic and flavonoid content were quantified with the 50% methanol extracts recorded the highest total phenolic content (5.76 ± 0.02 mg/g), 50% ethanol extracts had the highest total flavonoid content (4.38 ± 0.04 mg/g). Fingerprinting analysis via FTIR spectroscopy was used to identify phytochemicals confirming the presence of flavonoids and phenolics compounds in all extracts. The extracts were then introduced to a DPPH inhibition assay with the highest IC50 value obtained from 50% methanol with 75.2 ± 0.22 microgram per milliliter. Next, the antimicrobial capabilities were tested using a disc diffusion study on E. coli, B. subtilis, and Face normal flora showing that the methanolic extract has superior abilities in microbial inhibitions with the value recorded at 13mm, 22mm, and 14mm respectively. Overall, we obtained valuable pharmacognostic data on the harumanis leaves extracts as potential antioxidant and antimicrobial agents.

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Section: Total Phenolic and Flavonoid Contents Of Harumanis Leaves Ex...mentioning

confidence: 99%

Insights into the Pharmacognostic Elucidation of Harumanis Mango (Mangifera Indica Linn.) Leaves Extracts as Therapeutic Agent

Ryan Vitthaya Peron,

Amirul Ridzuan Abu Bakar,

Mohd Asraf Mohd Zainuddin

et al. 2024

ARMNE

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“…The content of flavonoids in mulberry was determined by the AlCl 3 chromogenic method [27]. First, a 0.2 g mulberry sample was accurately weighed by an analytical balance, and 5.0 mL 70% C 2 H 5 OH was added according to a material to liquid ratio of 1:25, and placed into a centrifuge tube.…”

Section: Content Of Functional Componentsmentioning

confidence: 99%

Effect of vacuum freeze drying and hot air drying on dried mulberry fruit quality

et al. 2023

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Two different drying methods (vacuum freeze-drying and hot-air drying) were used to dry mulberry of three varieties ’Baiyuwang’(D1), ’Longsang’(D2) and ’Zhongshen.1’(D3), and the fresh fruit of each variety was used as the control. The effects of different processing conditions on the physical characteristics, nutrients, functional components and antioxidant activity of mulberry fruit were analyzed. The results show that after different drying methods, after vacuum freeze-drying, the physical properties of dried mulberry fruit such as wettability, hygroscopic property and water retention, soluble protein, ascorbic acid and other nutrients, functional components such as polyphenols, resveratrol, chlorogenic acid and anthocyanin, and antioxidant activities such as DPPH free radical scavenging ability and ABTS free radical scavenging ability were superior to hot air drying (P < 0.01). It was concluded that vacuum freeze drying was more beneficial for retaining the original quality of mulberry than hot air drying. This study can provide a retaining theoretical basis for mulberry deep processing and comprehensive development and utilization.

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“…Total kandungan flavonoid sampel kemudian ditentukan berdasarkan ekuivalen dengan kurva standar kuersetin. Kuersetin digunakan sebagai standar karena termasuk golongan flavonoid dari gugus flavonol yang memiliki gugus keto pada C-4 dan memiliki gugus hidroksil pada atom C-3 atau C-5 yang berdekatan dari flavon dan flavonol (Matsuri et al, 2019). Berdasarkan Tabel 3, total kandungan flavonoid pada penelitian tertinggi yaitu Xylaria sp.…”

Section: Kandungan Total Flavonoid Cendawan Xylaria Spunclassified

Potensi Cendawan Xylaria sp. Sebagai Sumber Antioksidan

Prasetyo,

Listiyowati,

Batubara

2023

Al-Kauniyah J. Biol.

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AbstrakPencegahan radikal bebas di dalam tubuh dapat dilakukan dengan menggunakan antioksidan. Cendawan Xylaria memiliki kandungan senyawa bioaktif yang berasal dari metabolit sekunder yang berpotensi sebagai sumber antioksidan alami baru. Penelitian ini bertujuan menentukan potensi Xylaria sp. (strain F, D, C) sebagai sumber antioksidan melalui pengukuran aktivitas antioksidan dan kandungan total flavonoidnya. Cendawan ditumbuhkan pada media Potato Dextrose Yeast Extract Broth (PDYEB) dan diinkubasi 14 hari dengan kondisi gelap dan statis. Miselium cendawan digerus dengan bantuan nitrogen cair, kemudian ekstraksi dilakukan menggunakan pelarut metanol sebanyak dua kali ulangan. Penentuan aktivitas antioksidan menggunakan metode 2,2-Diphenyl-1-picrylhydrazyl (DPPH) dan kandungan total flavonoid ditentukan menggunakan metode alumunium klorida (AlCl3) yang dinyatakan ekuivalen kuersetin (QE). Seluruh sampel Xylaria sp. memiliki aktivitas antioksidan yang lemah dan kandungan flavonoid yang juga rendah. Xylaria sp. strain F memiliki aktivitas antioksidan tertinggi sebesar 1915,14 ± 24,73 µg/mL dan Xylaria sp. strain D memiliki kandungan total flavonoid tertinggi sebesar 2,41 ± 0,09 mg QE/g ekstrak. Senyawa flavonoid pada sampel Xylaria sp. tidak menjadi senyawa utama yang menunjukkan aktivitas antioksidannya.AbstractPrevention of free radicals in the body can be done by using antioxidants. Xylaria fungus contains bioactive compounds derived from secondary metabolites that have the potential as a source of new natural antioxidants. This study aims to determine the potential of Xylaria sp. (strains F, D, C) as a source of antioxidants by measuring their antioxidant activity and total flavonoid content. The fungus was grown on Potato Dextrose Yeast Extract Broth (PDYEB) and incubated for 14 days in dark and static conditions. The mycelium of the fungus was crushed with the help of liquid nitrogen, then the extraction was carried out using methanol as a solvent for two repetitions. Antioxidant activity was determined using the 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method and the total flavonoid content was determined using the alumunium chloride (AlCl3) method which is expressed as quercetin equivalent (QE). All samples of Xylaria sp. have the weakest antioxidant activity and lowest flavonoid content. Xylaria sp. strain F had the highest antioxidant activity of 1915,14 ± 24,73 µg/mL and Xylaria sp. strain D had the highest total flavonoid content of 2,41 ± 0,09 mg QE/g extract. The flavonoid compounds in the sample Xylaria sp. did not become the main compound showing antioxidant activity.

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Optimization of condition extraction in quantification of total flavonoid content in the seeds of the Arummanis (Mangifera indica L.) mango from Indonesia

Cited by 10 publications

References 9 publications

Insights into the Pharmacognostic Elucidation of Harumanis Mango (Mangifera Indica Linn.) Leaves Extracts as Therapeutic Agent

Insights into the Pharmacognostic Elucidation of Harumanis Mango (Mangifera Indica Linn.) Leaves Extracts as Therapeutic Agent

Effect of vacuum freeze drying and hot air drying on dried mulberry fruit quality

Potensi Cendawan Xylaria sp. Sebagai Sumber Antioksidan

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