Optimization of Conditions for Bacteriocin Extraction in PEG/Salt Aqueous Two‐Phase Systems Using Statistical Experimental Designs

Li, Chan; Bai, Jinghua; Li, Wei; Cai, Zhaoling; Ouyang, Fan

doi:10.1021/bp000167w

Cited by 47 publications

(32 citation statements)

References 20 publications

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“…The digestion time (A) is also showing the lowest effect (A, -0.0014) (Table 3) among all other factors and their interactions. The model presented correlation coefficient R 2 = 96.41 % and adjusted correlation coefficient R 2 (adj) = 93.28 %, which is in very good agreement (Li et al 2001;Jadhav et al 2012) between the experimental data and the model. Therefore, the model is statistically significant and predictable within the chosen variables.…”

Section: Screening Of Factorssupporting

confidence: 58%

“…As a result, the optimum value of the total sulphur measurement for KZK-1 is expected to present between the chosen ranges of the experimental variables; hence, it is not necessary to follow the steepest ascent technique for the optimization process (Li et al 2001). The screening of the investigated variables was performed by a regression model analysis to identify the factors with a significant effect on the response.…”

Section: Full Factorial Designmentioning

confidence: 99%

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Optimization of sample preparation method of total sulphur measurement in mine tailings

Khan

Shang

Alam

2013

Int. J. Environ. Sci. Technol.

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A sample preparation method of total sulphur measurement of reactive mine tailings was optimized. The total sulphur was measured by inductively coupled plasma optical emission spectroscopy, and ultrasound technique was used for sample digestion. The optimization process was adopted by a combined approach of experimental design and response surface methodology. The digestion time, temperature and acid-oxidant combination (i.e. effect of H 2 O 2 with a fixed amount of acid mixture) were investigated. A two-level and three-factor (2 3 ) full factorial design of experiment was applied to identify the most significant factors, and a central composite design was used to optimize the digestion procedure. KZK-1, a sericite schist, was selected as the certified reference material. The optimum methodology at 95 % confidence level (P \ 0.05) was identified to be 10 min of digestion at 77°C, with a solution of 1 ml HNO 3 :1 ml HCl:1.35 ml H 2 O 2 . This combination resulted in 100 % sulphur recovery. The investigated method was verified by X-ray diffraction analysis. The optimum digestion level was applied to a reactive mine tailings, which achieved satisfactory results with a percentage relative standard deviation \ 3 %.

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Section: Screening Of Factorssupporting

confidence: 58%

Section: Full Factorial Designmentioning

confidence: 99%

Optimization of sample preparation method of total sulphur measurement in mine tailings

Khan

Shang

Alam

2013

Int. J. Environ. Sci. Technol.

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“…That means, the output responses are situated in the vicinity of the optimal experimental region, thus the significant factors can be optimized by RSM in the next DoE step [7,14]. On the other hand, when the output responses are far away from the optimal experimental region no difference or curvature is existing, and therefore steepest ascent [16,18,23,24] or steepest descent [25] experiments have to be carried out in order to increase or decrease the output responses, respectively, thus reaching the proximity of the optimal experimental region and investigate the experimental direction [7,9]. These steeptest ascent/descent experiments are initially performed at the center-point of the significant factors and each factor level is increased or decreased according to the magnitude of the main effects [7].…”

Section: Steepest Ascent / Descent Methodsmentioning

confidence: 99%

“…12 has been frequently set to ± 1.41 [6,18,23,35,38,[46][47][48][49] for the optimization of PEG-salt ATPS. Hence, the CCC design for three factors is obviously represented by a hyperspherical experimental arrangement due to corner and axial experiments approximating a spherical surface (Fig.…”

Section: Accepted M Manuscriptmentioning

confidence: 99%

Optimization of PEG–salt aqueous two-phase systems by design of experiments

Glyk

Solle

Scheper

et al. 2015

Chemometrics and Intelligent Laboratory Systems

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Since the mechanism governing the partitioning behavior of biomolecules, such as proteins and enzymes, in polyethylene glycol (PEG)-salt aqueous two-phase systems (ATPS) is complex and not easily predictable, many laborious experiments have to be performed for an optimization of these systems, causing increased overall cost. However, the multivariate statistical design of experiments (DoE) methodology is representing a promising and efficient optimization technique which can overcome the limitations of traditional optimization methods. Therefore, DoE has emerged as a powerful and efficient optimization tool for PEGsalt ATPS, since it is faster, more efficient and cost-effective, allowing a simultaneous and rigorous evaluation of process/system parameters. In the present review, different DoE process steps are represented to highlight the feasibility of this approach to operate as a promising and efficient optimization tool, thus facilitating the evaluation of the partitioning behavior, recovery and purification of different proteins and enzymes in PEG-salt ATPS. In this context, several experimental designs, such as factorial and response surface designs, have been discussed and evaluated by statistical regression analysis and analysis of variance (ANOVA), as well as various applications of PEG-salt ATPS using DoE have been outlined which may further promote the optimization of these systems.

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“…Bacteriocins of LAB are cationic, hydrophobic and present high isoeletric point that facilitates the extraction from the producers cells, depending on pH (9).…”

mentioning

confidence: 99%

Improved adsorption-desorption extraction applied to the partial characterization of the antilisterial bacteriocin produced by Carnobacterium maltaromaticum C2

Tulini¹,

Martinis²

2010

Braz. J. Microbiol.

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Bacteriocins are ribosomally produced peptides useful for food biopreservation. An improved adsorptiondesorption process is proposed for the partial purification of the bacteriocin produced by the fish isolate Carnobacterium maltaromaticum C2. Analyzis of extract by SDS-PAGE indicated this method may offer an alternative to improve the yield of purification of bacteriocins. Bacteriocin extraction was done in a two-steps approach,

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Optimization of Conditions for Bacteriocin Extraction in PEG/Salt Aqueous Two‐Phase Systems Using Statistical Experimental Designs

Cited by 47 publications

References 20 publications

Optimization of sample preparation method of total sulphur measurement in mine tailings

Optimization of sample preparation method of total sulphur measurement in mine tailings

Optimization of PEG–salt aqueous two-phase systems by design of experiments

Improved adsorption-desorption extraction applied to the partial characterization of the antilisterial bacteriocin produced by Carnobacterium maltaromaticum C2

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