Optimization of Conditions for Blood Plasma Peptidome Analysis

Ziganshin, Rustam H.; Ryabinin, V. V.; Azarkin, I. V.; Govorun, Vadim M.; Иванов, В. Т.

doi:10.1134/s1068162018030159

Cited by 2 publications

(1 citation statement)

References 21 publications

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“…To analyze the biochemical composition of the ECM, the obtained decellularized liver tissue was treated by enzymes [45]. Peptides were separated on an Ultimate 3000 Nano LC System chromatographic system (Thermo Fisher Scientific, USA) coupled to a Q Exactive HF mass spectrometer (Thermo Fisher Scientific, USA) using a nanoelectrospray source (Thermo Fisher Scientific, USA).…”

Section: Mass Spectrometry Analysis Of Biochemical Composition Of Dec...mentioning

confidence: 99%

Relation between micro- and nanostructure features and biological properties of the decellularized rat liver

Bobrova¹,

Safonova²,

Ефимов³

et al. 2021

Biomed. Mater.

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Organ decellularization is one of the promising technologies of regenerative medicine, which allows obtaining cell-free extracellular matrix (ECM), which provide preservation of the composition, architecture, vascular network and biological activity of the ECM. The method of decellularization opens up wide prospects for its practical application not only in the field of creating full-scale bioengineered structures, but also in the manufacture of vessels, microcarriers, hydrogels, and coatings. The main goal of our work was the investigation of structure and biological properties of lyophilized decellularized Wistar rat liver fragments (LDLFs), as well as we assessed the regenerative potential of the obtained ECM. We obtained decellularized liver of a Wistar rat, the vascular network and the main components of the ECM of tissue were preserved. H&E staining of histological sections confirmed the removal of cells. DNA content of ECM is equal to 0.7% of native tissue DNA content. Utilizing scanning probe nanotomogrphy method, we showed sinuous, rough topography and highly nanoporous structure of ECM, which provide high level of mouse 3T3 fibroblast and Hep-G2 cells biocompatibility. Obtained LDLF had a high regenerative potential, which we studied in an experimental model of a full-thickness rat skin wound healing: we observed the acceleration of wound healing by 2.2 times in comparison with the control.

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Section: Mass Spectrometry Analysis Of Biochemical Composition Of Dec...mentioning

confidence: 99%