Optimization of conditions for cultivation of pathogens of infectious rhinotracheitis and viral diarrhea

Abstract

This article reflects the results of research on optimizing the conditions of cultivation of strain “ТК-A(VIEV)-В2” of infectious bovine rhinotracheitis (IBR) and strain “ВК-1” of bovine viral diarrhea (BVDV). It was found that the roller cultivation method is most easily applicable and provides the production of viral material with high infectious activity. A comparative analysis of the results of studying the sensitivity of continuous cell cultures to the pathogens of IBR and BVDV of cattle of the latter made it possible to select the MDBK cell line to obtain the maximum titer of viruses. The optimal dose for infecting cell culture with both pathogens was the content of viral particles from 0.2 to 0.5 TCD50/cm3 per cell. The maximum reproduction of viruses was observed using a culture concentration of cells from 110 to 120 thousand cells/cm3 and the duration of their cultivation for 2-3 days. The results obtained can be applied for the further development and creation of preventive and diagnostic tools for IBR and BVDV of cattle.