Optimization of Conditions for Extraction of Pepsin-Soluble Collagen from Indonesian Local “Kacang” Goatskin by Response Surface Methodology

Wahyuningsih, Rina; Rusman,; Nurliyani, Nurliyani; Pertiwiningrum, Ambar; Rohman, Abdul; Fitriyanto, Nanung Agus; Erwanto, Y.

doi:10.3844/ajavsp.2018.70.75

Cited by 7 publications

(5 citation statements)

References 18 publications

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“…Further findings on the amino acid analysis indicate that out of the 13 amino acids identified by HPLC, glycine composition was 37.37%. Normally, a typical collagen is composed of a glycine-prolinehydroxyproline sequence whereby the composition of glycine dominates the chain (Nurilmala et al, 2019;Wahyuningsih et al, 2018). Therefore, the amount of glycine obtained in this study confirms the extracted product is collagen.…”

Section: Amino Acid Compositionsupporting

confidence: 74%

Extraction and Characterization of Collagen from Cattle Horns for Potential Wound Healing

Halson

Haule

Nambela

et al. 2023

TJET

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The need for bandages and gauze for wound dressing and healing is inevitable due to the high increase in accidents. Most of the material used for the production of bandages and gauzes for wound healing are from non-renewable synthetic material. The sustainability of nonrenewable resources is at jeopardy due to the health and associated environmental impact. Consequently, the use of naturally occurring and biodegradable material is a promising solution to the sustainability challenges of synthetic material. In this paper, the extraction of collagen films from cattle horns for potential wound healing is reported. Collagen was extracted from cattle horns by acid soluble method. The maximum yield of collagen was 12% (on dry weight basis). The physicochemical characterization was carried out to confirm the purity and structural integrity of the extracted collagen. Based on the SDS-PAGE analysis, the estimated molecular weight of the α chain and β chain were approximately 135 kDa and 245 kDa respectively. UV/Vis absorption peak at 220 nm indicated that the collagen produced was type I collagen. FTIR spectra showed regions of amides A,

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Section: Amino Acid Compositionsupporting

confidence: 74%

Extraction and Characterization of Collagen from Cattle Horns for Potential Wound Healing

Halson

Haule

Nambela

et al. 2023

TJET

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“…Collagen hydrolysis using the method by [8] with slight modification. The best acid-soluble collagen (ASC) in the previous study was hydrolyzed using collagen [9]. [A single gram of ASC sample was dissolved in 100 milliliters of buffer (pH 2.0) and incubated for 15 minutes at 37°C.…”

Section: Hydrolysis Of Collagenmentioning

confidence: 99%

Acid-soluble collagen (ASC) hydrolysate from Javanese goat (Capra aegagrus hocus) skin: effect of pepsin hydrolysis and antioxidant activity

Hakim,

Ujilestari,

Rusman

et al. 2024

IOP Conf. Ser.: Earth Environ. Sci.

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Javanese goat (Capra aegagrus hocus) is an animal species which have many populations in Indonesia and it is used because more selective in feed, especially Leguminosae and seeds which of rich in collagen. The study aims to determine the impact of hydrolysis time and antioxidant activities on the extraction of skin Javanese goats using the acid-soluble collagen (ASC) method. The ASC used in this investigation was taken from another study and hydrolyzed with pepsin throughout the course of 0, 30, 60, 90, and 120 minutes of incubation. The success of the hydrolysis process can be shown by the percent degree of hydrolysis. Methods are used to determine the percent degree of hydrolysis of the TCA method. Results showed that hydrolyzing for 60 min yields the highest total soluble collagen of ASC, with a value of 0.77 mg/ml. Finally, the ASC which is hydrolysis use pepsin for 60 min has a degree of hydrolysis, and total soluble collagen is the highest. The protein molecular weight of ASC which is the hydrolysis of 60 min is smaller than before hydrolysis and it has radical scavenging activity of 56.42% ± 1.45. So far, ASC from Javanese goat (Capra aegagrus hocus) has been hydrolyzed in this study has high antioxidant activity and can be recommended as a substitute for functional food.

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“…A two-step extraction process without a preceding treatment has been reported for recovery of collagen from sheepskin; an enzymatic step was used first and this was followed by acid hydrolysis [ 79 ]. Another hybrid extraction procedure using acetic acid in combination with pepsin has been described by several authors [ 55 , 56 , 59 , 60 , 62 , 68 , 80 , 81 , 82 , 83 , 84 ].…”

Section: Collagen Extraction Processmentioning

confidence: 99%

“…Salts are most often used to achieve this, with a salt concentration exceeding 1.0 kmol m −3 . This treatment has been used for collagen from chicken skin [ 62 , 63 , 80 , 93 ], sheep and goatskin [ 54 , 55 , 68 , 81 ], pigskin [ 82 , 94 ], and cattle skin [ 59 , 95 ].…”

Section: Collagen Extraction Processmentioning

confidence: 99%

Collagen Extraction from Animal Skin

Matinong

Chisti

Pickering

et al. 2022

Biology

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Collagen is the most abundant structural protein in animals. It is the major component of skin. It finds uses in cosmetics, medicine, yarn production and packaging. This paper reviews the extraction of collagen from hides of most consumed animals for meat with the focus on literature published since 2000. The different pretreatment and extraction techniques that have been investigated for producing collagen from animal skins are reviewed. Pretreatment by enzymatic, acid or alkaline methods have been used. Extraction by chemical hydrolysis, salt solubilization, enzymatic hydrolysis, ultrasound assisted extraction and other methods are described. Post-extraction purification methods are also explained. This compilation will be useful for anyone wishing to use collagen as a resource and wanting to further improve the extraction and purification methods.

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Optimization of Conditions for Extraction of Pepsin-Soluble Collagen from Indonesian Local “Kacang” Goatskin by Response Surface Methodology

Cited by 7 publications

References 18 publications

Extraction and Characterization of Collagen from Cattle Horns for Potential Wound Healing

Extraction and Characterization of Collagen from Cattle Horns for Potential Wound Healing

Acid-soluble collagen (ASC) hydrolysate from Javanese goat (Capra aegagrus hocus) skin: effect of pepsin hydrolysis and antioxidant activity

Collagen Extraction from Animal Skin

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