Optimization of conditions for the production of laccase by Trametes villosa (Sw.) Kreisel and its application in the bio-treatment of sugar cane vinasse

Abstract

Se optimizaron condiciones para la producción de lacasa por Trametes villosa mediante la metodología de superficie de respuesta: relación C:N, nivel de Cu+2 (mM) y tiempo de fermentación. El extracto crudo se inoculó en vinaza diluida al 25% y se evaluó el cambio en algunos parámetros de calidad de agua residual. La optimización produjo un aumento en la actividad de lacasa de 2,86 veces con respecto al medio no optimizado. Se observó reducción en los valores de DQO, fenoles y color en la vinaza tratada de 46,33%, 73,98% y 52,87% respectivamente. El pH aumentó de 6,50 a 6,57 y la actividad enzimática mostró un marcado aumento en medio con vinaza. Se confirma la utilidad de la metodología de superficie de respuesta en la optimización de condiciones en procesos de fermentación. Selected conditions for laccase production by Trametes villosa were optimized by surface response methodology: C:N ratio, Cu+2 level (mM), and fermentation time. The crude extract was inoculated on 25% (v/v) diluted sugarcane vinasse and some residual water quality parameters were evaluated. A 2.86-fold increase in the enzymatic activity was obtained upon optimization. Reduction of COD, phenol and color values on the treated vinasse were 46.33%, 73.98% and 52.87% respectively. pH increased from 6.50 to 6.57 and laccase enzymatic activity in vinasse media increased considerably. The advantage of surface respond methodologies for optimization of fermentation conditions is confirmed.

An Optimal Culture Medium for Laccase Production and Sugar Cane Vinasse Biotreatment with Trametes villosa Using Plackett-Burman and Central Composite Designs

“…The inoculum volume, the initial pH, and the dilution of vinasse were also evaluated in the screening experiment. Culture conditions such as temperature (33.6 °C), agitation speed (120 rpm), and light (alternate periods of 12 h), were established according to Caballero et al [24], De Gracia and Navarro [25] and Santos [26].…”