Optimization of Cu and Zn co-doped PEO titania coatings produced in a novel borate-based electrolyte for biomedical applications

Pavarini, Matteo; Moscatelli, Marco; Nardo, Luigi De; Chiesa, Roberto

doi:10.1016/j.matlet.2021.129627

Cited by 4 publications

(4 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Copper and zinc ions are recognized as antibacterial agents for such purposes, especially because they decrease the adhesion of bacteria and the formation of biofilms. Copper- and zinc-based compounds were successfully incorporated into an oxide layer on a Ti surface when the PEO process was carried out in anodizing bath containing Zn(CH 3 COO) 2 and Cu(CH 3 COO) 2 [ 149 ].…”

Section: Control Strategies For the Formation Of Clinically Important...mentioning

confidence: 99%

Recent Advances in the Control of Clinically Important Biofilms

Krukiewicz

Kazek-Kęsik

Brzychczy-Włoch

et al. 2022

IJMS

View full text Add to dashboard Cite

Biofilms are complex structures formed by bacteria, fungi, or even viruses on biotic and abiotic surfaces, and they can be found in almost any part of the human body. The prevalence of biofilm-associated diseases has increased in recent years, mainly because of the frequent use of indwelling medical devices that create opportunities for clinically important bacteria and fungi to form biofilms either on the device or on the neighboring tissues. As a result of their resistance to antibiotics and host immunity factors, biofilms have been associated with the development or persistence of several clinically important diseases. The inability to completely eradicate biofilms drastically increases the burden of disease on both the patient and the healthcare system. Therefore, it is crucial to develop innovative ways to tackle the growth and development of biofilms. This review focuses on dental- and implant-associated biofilm infections, their prevalence in humans, and potential therapeutic intervention strategies, including the recent advances in pharmacology and biomedical engineering. It lists current strategies used to control the formation of clinically important biofilms, including novel antibiotics and their carriers, antiseptics and disinfectants, small molecule anti-biofilm agents, surface treatment strategies, and nanostructure functionalization, as well as multifunctional coatings particularly suitable for providing antibacterial effects to the surface of implants, to treat either dental- or implant-related bacterial infections.

show abstract

Section: Control Strategies For the Formation Of Clinically Important...mentioning

confidence: 99%

Recent Advances in the Control of Clinically Important Biofilms

Krukiewicz

Kazek-Kęsik

Brzychczy-Włoch

et al. 2022

IJMS

View full text Add to dashboard Cite

show abstract

“…aureus and Pseudomonas aeruginosa at a Zn ion concentration below 0.5 ppm after 24 h . The morphology and chemical composition of Cu- and Zn-codoped PEO coatings were optimized by regulating process parameters . Zn-containing TiO 2 coating exhibited bactericidal activity against both Gram-negative E.…”

Section: Introductionmentioning

confidence: 95%

“…For example, Zn-incorporated PEO coatings showed a minor bactericidal effect against S. aureus and Pseudomonas aeruginosa at a Zn ion concentration below 0.5 ppm after 24 h. 37 The morphology and chemical composition of Cu-and Zn-codoped PEO coatings were optimized by regulating process parameters. 38 Zn-containing TiO 2 coating exhibited bactericidal activity against both Gram-negative E. coli and Gram-positive S. aureus a little over 90%, which is not enough. 39 The antibacterial tests of Ca-, P-, and Znincorporated PEO coatings revealed low antibacterial ability toward S. aureus and slightly higher activity against E. coli.…”

Section: Introductionmentioning

confidence: 97%

Synergistic Bactericidal Effect of Zn²⁺ Ions and Reactive Oxygen Species Generated in Response to Either UV or X-ray Irradiation of Zn-Doped Plasma Electrolytic Oxidation TiO₂ Coatings

Popova,

Advakhova,

Sheveyko

et al. 2024

ACS Appl. Bio Mater.

View full text Add to dashboard Cite

Zn-containing TiO 2 -based coatings with Na, Ca, Si, and K additives were obtained by plasma electrolytic oxidation (PEO) of Ti in order to achieve an effective and broad bactericidal protection without compromising biocompatibility. A protocol has been developed for cleaning the coating surface from electrolyte residues, ensuring the preservation of the microstructure and composition of the surface layer. Using high-resolution transmission electron microscopy, three characteristic microstructural zones in the PEO-Zn coating are well documented: zone 1 with a TiO 2 -based nanocrystalline structure, zone 2 with an amorphous structure, and zone 3 around pores with an amorphous-nanocrystalline structure. The excellent cytocompatibility of PEO-Zn samples was confirmed by three different methods: monitoring the proliferation of MC3T3-E1 cells, assessing the viability of sheep osteoblast cells using calcein-AM staining and fluorescence microscopy, and incubation with spheroids based on primary osteoblast cells and mouse embryonic fibroblast NIH3T3 cells. The PEO-Zn coatings absorb >60% of the incident light over the UV and Vis-NIR spectral ranges. After 24 h, the PEO-Zn coatings completely inactivate four types of strains: Gram-positive Staphylococcus aureus CSA154 and ATCC29213 and Gram-negative Escherichia coli K261 and U20, and also prevent E. coli U20 and K261 biofilm formation. The superior antibacterial activity is associated with the synergistic effect of Zn 2+ ions in safe concentration and reactive oxygen species (ROS) generated in response to either UV irradiation or soft short-term X-ray irradiation. The X-ray irradiationinduced ROS formation by a PEO coating is reported for the first time. The enhanced bactericidal activity after X-ray irradiation compared to UV illumination is attributed to the more intense ROS generation in the first few hours. The results obtained significantly expand the possibilities of using PEO coatings on the surfaces of titanium implants.

show abstract

“…Silver is one of the most well-known antibacterial agents and has been added to the electrolyte composition to obtain an Ag-containing PEO coating . Another promising bactericidal element is copper (Cu), whose ions exhibit a strong bactericidal effect against Gram-positive and Gram-negative bacteria at concentrations that are safe for the body. − Cu is being studied as an antibacterial additive to various materials, such as Ti-, Co-, or Mg-based alloys, stainless steel, calcium phosphate cement, and others. − PEO coatings were subjected to Cu 2+ ion implantation, which, however, significantly complicates the process .…”

Section: Introductionmentioning

confidence: 99%

Osteoconductive, Osteogenic, and Antipathogenic Plasma Electrolytic Oxidation Coatings on Titanium Implants with BMP-2

Popova,

Sheveyko,

Kuptsov

et al. 2023

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

We report a one-pot plasma electrolytic oxidation (PEO) strategy for forming a multi-element oxide layer on the titanium surface using complex electrolytes containing Na 2 HPO 4 , Ca(OH) 2 , (NH 2 ) 2 CO, Na 2 SiO 3 , CuSO 4 , and KOH compounds. For even better bone implant ingrowth, PEO coatings were additionally loaded with bone morphogenetic protein-2 (BMP-2). The samples were tested in vivo in a mouse craniotomy model. Tests for bactericidal and fungicidal activity were carried out using clinically isolated multi-drug-resistant Escherichia coli (E. coli) K261, E. coli U20, methicillin-resistant Staphylococcus aureus (S. aureus) CSA154 bacterial strains, and Neurospora crassa (N. crassa) and Candida albicans (C. albicans) D2528/20 fungi. The PEO-Cu coating effectively inactivated both Gram-positive and Gram-negative bacteria at low concentrations of Cu 2+ ions: minimal bactericidal concentration for E. coli and N. crassa (99.9999%) and minimal inhibitory concentration (99.0%) for S. aureus were 5 ppm. For all studied bacterial and fungal strains, PEO-Cu coating completely prevented the formation of bacterial and fungal biofilms. PEO and PEO-Cu coatings demonstrated bone remodeling and moderate osteoconductivity in vivo, while BMP-2 significantly enhanced osteoconduction and osteogenesis. The obtained results are encouraging and indicate that Ti-based materials with PEO coatings loaded with BMP-2 can be widely used in customized medicine as implants for orthopedics and craniomaxillofacial surgery.

show abstract

Optimization of Cu and Zn co-doped PEO titania coatings produced in a novel borate-based electrolyte for biomedical applications

Cited by 4 publications

References 10 publications

Recent Advances in the Control of Clinically Important Biofilms

Recent Advances in the Control of Clinically Important Biofilms

Synergistic Bactericidal Effect of Zn²⁺ Ions and Reactive Oxygen Species Generated in Response to Either UV or X-ray Irradiation of Zn-Doped Plasma Electrolytic Oxidation TiO₂ Coatings

Osteoconductive, Osteogenic, and Antipathogenic Plasma Electrolytic Oxidation Coatings on Titanium Implants with BMP-2

Contact Info

Product

Resources

About

Optimization of Cu and Zn co-doped PEO titania coatings produced in a novel borate-based electrolyte for biomedical applications

Cited by 4 publications

References 10 publications

Recent Advances in the Control of Clinically Important Biofilms

Recent Advances in the Control of Clinically Important Biofilms

Synergistic Bactericidal Effect of Zn2+ Ions and Reactive Oxygen Species Generated in Response to Either UV or X-ray Irradiation of Zn-Doped Plasma Electrolytic Oxidation TiO2 Coatings

Osteoconductive, Osteogenic, and Antipathogenic Plasma Electrolytic Oxidation Coatings on Titanium Implants with BMP-2

Contact Info

Product

Resources

About

Synergistic Bactericidal Effect of Zn²⁺ Ions and Reactive Oxygen Species Generated in Response to Either UV or X-ray Irradiation of Zn-Doped Plasma Electrolytic Oxidation TiO₂ Coatings