Optimization of Cultural Conditions for Production of Chitinase by a Soil Isolate of Massilia timonae

Faramarzi, Mohammad Ali; Fazeli, Mehdi; Yazdi, Mojtaba Tabatabaei; Adrangi, Sina; Al-Ahmadi, K. J.; Tasharrofi, Noshin; Mohseni, F. A.

doi:10.3923/biotech.2009.93.99

Cited by 61 publications

(43 citation statements)

References 23 publications

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“…Intriguingly, there was also an increase in abundance of Massilia OTUs. Massilia is able to degrade chitin (Faramarzi et al, 2009;Adrangi et al, 2010) found in the fungus cell wall (James et al, 2006). It may also explain the repeated observation that Massilia is abundant in the rhizosphere (Bodenhausen et al, 2013) and found to be associated with soils colonised by pathogenic fungi like Fusarium and Rhizoctonia solani (Klein et al, 2013;Yin et al, 2013).…”

Section: Succession Of the Rhizosphere Microbiotamentioning

confidence: 74%

“…There was also a similar increase in abundance of the bacterial genus Massilia, suggesting it may be related to this invasion. Since this genus grows on chitin as a carbon and nitrogen source (Faramarzi et al, 2009;Adrangi et al, 2010) there may be a causal link, with Massilia growing on material derived from the putative O. brassicae (Figure 4) (James et al, 2006).…”

Section: Microbiota Selection In Compostmentioning

confidence: 99%

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Stability and succession of the rhizosphere microbiota depends upon plant type and soil composition

et al. 2015

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We examined succession of the rhizosphere microbiota of three model plants (Arabidopsis, Medicago and Brachypodium) in compost and sand and three crops (Brassica, Pisum and Triticum) in compost alone. We used serial inoculation of 24 independent replicate microcosms over three plant generations for each plant/soil combination. Stochastic variation between replicates was surprisingly weak and by the third generation, replicate microcosms for each plant had communities that were very similar to each other but different to those of other plants or unplanted soil. Microbiota diversity remained high in compost, but declined drastically in sand, with bacterial opportunists and putative autotrophs becoming dominant. These dramatic differences indicate that many microbes cannot thrive on plant exudates alone and presumably also require carbon sources and/or nutrients from soil. Arabidopsis had the weakest influence on its microbiota and in compost replicate microcosms converged on three alternative community compositions rather than a single distinctive community. Organisms selected in rhizospheres can have positive or negative effects. Two abundant bacteria are shown to promote plant growth, but in Brassica the pathogen Olpidium brassicae came to dominate the fungal community. So plants exert strong selection on the rhizosphere microbiota but soil composition is critical to its stability. microbial succession/ plant-microbe interactions/rhizosphere microbiota/selection.

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Section: Succession Of the Rhizosphere Microbiotamentioning

confidence: 74%

Section: Microbiota Selection In Compostmentioning

confidence: 99%

Stability and succession of the rhizosphere microbiota depends upon plant type and soil composition

et al. 2015

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“…One ose of bacterial culture (2 x 24 hours old) was taken and placed on object glass, then was dripped by one drop of KOH solution (3%). If clumping occurs, the bacteria is gram-negative and if not it is grampositive bacteria [4].…”

Section: B Characterization Of Physiology Of Indigenous Rb Isolatesmentioning

confidence: 99%

Characterization of Indigenous Rhizobacterial Isolates from Healthy Chilli Rhizosphere Capable of Inducing Resistance Against Anthracnose Disease (Colletotrichum Gloeosporioides).

Fatimah¹,

Habazar²,

Аrbаin³

et al. 2014

International Journal on Advanced Science, Engineering and Information Technology

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Abstract-Antrachnose disease on chilli caused by Colletotrichum gloeosporioides is difficult to be controlled because the disease can be transmitted through the seeds, and has a high genetic diversity. One of promising alternative control is using biological control agents, such as groups of rhizobacteria. The objective of this research were : to characterize the morphology, physiology and molecular of selected rhizobacterial isolates, which were capable of controlling the anthracnose disease and to enhance the growth and yield chilli. Three rhizobacterial isolates (B1.37, B2.11 and P1.31) were used. These isolates were indentified based on morphology (colony form, elevation, edge, and color), physiology (gram tes, the production of hormone IAA, chitinase enzyme, hydrogen cyanide, and solvents phosphate) and molecular. The isolates were identified by using 16S rRNA sequencing. The results indicated that isolate B1.37 belonged to species of Bacillus cereus strain ML 267, isolate B2.11 belonged to Bacillus cereus strain LH8 and isolate P1.31 belonged to Chryseobacterium gleum strain NBRC 15054.

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“…1) and put on medium agar surface supplemented with 1% colloidal chitin and incubated at 30˚C [5]. Chitinolytic index data (ratio of clear zone and colony sizediameter) were recorded up to 7 days incubations [11]. Data of chitinolytic index were analyzed using analysis of variance (ANOVA) that continued with Games-Howel test (α = 0.05) using SPSS for Windows V.16.…”

Section: Primary Screening Of Chitinolytic Bacteriamentioning

confidence: 99%

“…Colloidal chitin was prepared according modified method as described by Faramarzi et al [11]. Ten grams of chitin from shrimp shell flake were added into 100 ml concentrated HCl (37%) and kept in vigorous stirring for 2 h at room temperature or until chitin completely dissolved.…”

Section: Preparation Of Colloidal Chitinmentioning

confidence: 99%

Chitinolytic Assay and Identification of Bacteria Isolated from Shrimp Waste Based on 16S rDNA Sequences

Setia¹,

Suharjono²

2015

AiM

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Shrimp waste contains 20% -60% chitin and possible to be source of chitinolytic bacteria. Chitinolytic bacteria are capable of hydrolyzing of chitin progressively to produce N-acetylglucosamine monomer which can be used to overcome the shrimp waste. The objectives of this research were to identify species of bacteria with high activity of chitin degradation in shrimp waste and to analyze their potency as chitin degradation agent. The research consists of screening of chitinolytic bacteria based on chitinolytic index, activity assay of chitinase using colorimetric method, and molecular identification of bacteria based on 16S rDNA sequences. Two of eighteen isolates of chitinolytic bacteria (PBK 2 and SA 1.2 isolates) showed the highest chitinolytic index, which were 2.069 and 2.084, whereas chitinase activity was 0.213 and 0.219 U/ml respectively. Based on 16S rDNA sequences, isolate of PBK 2 was identified as Acinetobacter johnsonii 3-1, whereas SA 1.2 was identified as Bacillus amyloliquefaciens GR53 with 99.78% similarity.

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Optimization of Cultural Conditions for Production of Chitinase by a Soil Isolate of Massilia timonae

Cited by 61 publications

References 23 publications

Stability and succession of the rhizosphere microbiota depends upon plant type and soil composition

Stability and succession of the rhizosphere microbiota depends upon plant type and soil composition

Characterization of Indigenous Rhizobacterial Isolates from Healthy Chilli Rhizosphere Capable of Inducing Resistance Against Anthracnose Disease (Colletotrichum Gloeosporioides).

Chitinolytic Assay and Identification of Bacteria Isolated from Shrimp Waste Based on 16S rDNA Sequences

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