Optimization of Cultural Conditions for<i> Bacillus megaterium</i> Cultured in<i> Agaricus bisporus</i> Industrial Wastewater

Huang, Jinhong; Zhang, Danfeng; Ou, Yixin; Zhang, Guoguang; Zheng, Linhua; Lin, Lizhuan; Ye, Xiaomei; Zhu, Xiaofeng; Pan, Yutian

doi:10.1155/2018/8106245

Cited by 5 publications

(3 citation statements)

References 19 publications

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“…Even though the concentration of industrial wastewater was low, it was suit for microorganism growth. For example, 0.25% concentration of industrial wastewater was suitable to support B. mucilaginous growth (Huang et al, 2018b), and 1% concentration was suit for B. megaterium (Huang et al, 2018a). Comparing with B. mucilaginous and B. megaterium, the growth of S. cerevisiae needed more nutrition that higher concentration of industrial wastewater supported in the study.…”

Section: Verification Testmentioning

confidence: 86%

“…In the canning production process, the fresh mushrooms are precooked firstly, in which the weight is reduced by 35% ~40%, in other words, at least about 30% of A. bisporus production will be lost to industrial wastewater each year (Huang et al, 2016). However, the mushroom industrial wastewater has high BOD and COD content, of which the content of COD is 540.29 g/L, which is 13.07 times higher than the national three level emission standard (Huang et al, 2018a). The discharge of the wastewater into the surrounding areas is a cause of environmental pollution.…”

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confidence: 99%

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Plackett-Burman design and response surface optimization of conditions for culturing Saccharomyces cerevisiae in Agaricus bisporus industrial wastewater [pdf]

Huang¹,

Zhang²,

Zheng³

et al. 2019

Acta Sci Pol Technol Aliment

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Background. The Agaricus bisporus industrial wastewater that contains a variety of nutrients which could be used as culture for some beneficial microbiology will be one threat to our environment if the wastewater doesn't be comprehensively utilized. Plackett-Burman is the rapid and concise ways of screening the main effective factors. Box-Behnken response surface method is used to optimize interactions between the three main factors and predict optimal fermentation conditions. This study is aimed to select the main influence factors and optimize the conditions for culturing Saccharomyces cerevisiae in A. bisporus industrial wastewater by Plackett-Burman design and Box-Behnken response surface method. Material and methods. We analyzed the total number of living S. cerevisiae in the fermentation broth using multispectral imaging flow cytometry. Plackett-Burman design was used to screen out three factors from the original six factors of processing wastewater concentration, initial pH, inoculum size, liquid volume, culture temperature, and rotation speed that affected the total number of viable S. cerevisiae. Factors significantly affecting the total number of viable S. cerevisiae, including culturing temperature, processing wastewater concentration, and initial pH were investigated. Result. The results indicated that culture temperature (p = 0.0007) and pH (p = 0.0344) as negative factor and concentration (p = 0.0080) as positive effect were the significant factors affecting the total number of S. cerevisiae, inoculum (p = 0.1237) and shaking speed (p = 0.2112) as positive effect and loaded liquid (p = 0.4811) as negative factor were important factors. The optimum conditions for S. cerevisiae fermentation in A. bisporus wastewater were a rotational speed of 150 rpm, a culture temperature of 25°C, an initial pH of 6.0, a concentration of 8.4%, a inoculation volume of 8%, and a 100 mL liquid volume in a 250 mL flask, a culture time of 48 h. Under these conditions, the concentration of total viable yeast reached 1.04 ±0.02 × 10 8 Obj/mL which was at the 95% confidence interval of predicted model (0.89-1.14 × 10 8 Obj/mL). Conclusion. The experimental model is reliable and the experimental results are of good stability. Variance analysis is performed to determine the adequacy and significance of the linear model. Thus, Plackett-Burman This study was funded by the Fujian Provincial Department of Science and Technology (no. 2019N0018), Natural Science Fund Project of Fujian Province (no. 2015J01144) and the young teacher project of Fujian Provincial Department of Education (no. JT180297 and no. JT180306). Preparation of seed suspension Activated S. cerevisiae was picked with a vaccination loop and transferred to 100 mL sterilized seed liquid medium. This yeast suspension was placed in a 28°C incubator with shaking at 150 rpm for 48 h. Plackett-Burman design In this study, the experimental design included 6 factors and the experimental number selected was N = 12. A, B, C, D, E, and F respectively represented the industrial...

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Section: Verification Testmentioning

confidence: 86%

mentioning

confidence: 99%

Plackett-Burman design and response surface optimization of conditions for culturing Saccharomyces cerevisiae in Agaricus bisporus industrial wastewater [pdf]

Huang¹,

Zhang²,

Zheng³

et al. 2019

Acta Sci Pol Technol Aliment

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“…Mutations in CBS genes are implicated in colon and ovarian cancer 3 – 5 and infertility 6 . In particular, CBS is overexpressed in ovarian cancer and contributes to advanced cancer progression and chemoresistance.…”

Section: Introductionmentioning

confidence: 99%

Functional characterization of Caenorhabditis elegans cbs-2 gene during meiosis

Santonicola

Germoglio

d'Abbusco

et al. 2020

Sci Rep

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Cystathionine β-synthase (CBS) is a eukaryotic enzyme that maintains the cellular homocysteine homeostasis and catalyzes the conversion of homocysteine to L-cystathionine and Hydrogen sulfide, via the trans-sulfuration pathway. In Caenorhabditis elegans, two cbs genes are present: cbs-1 functions similarly as to human CBS, and cbs-2, whose roles are instead unknown. In the present study we performed a phenotypic characterization of the cbs-2 mutant. The null cbs-2 mutant is viable, fertile and shows the wild-type complement of six bivalents in most oocyte nuclei, which is indicative of a correct formation of crossover recombination. In absence of synaptonemal complex formation (syp-2 mutant), loss of cbs-2 leads to chromosome fragmentation, suggesting that cbs-2 is essential during inter-sister repair. Interestingly, although proficient in the activation of the DNA damage checkpoint after exposure to genotoxic stress, the cbs-2 mutant is defective in DNA damage-induced apoptosis in meiotic germ cells. These results suggest possible functions for CBS-2 in meiosis, distinct from a role in the trans-sulfuration pathway. We propose that the C. elegans CBS-2 protein is required for both inter-sister repair and execution of DNA damage-induced apoptosis.

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