Optimization of cultural conditions for enhancement of anti-quorum sensing potential in the probiotic strain Lactobacillus rhamnosus GG against Pseudomonas aeruginosa

Devi, Surekha; Chhibber, Sanjay; Harjai, Kusum

doi:10.1007/s13205-022-03187-2

Cited by 2 publications

(2 citation statements)

References 73 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The MICs were determined using the broth microdilution method [ 24 , 25 ]. Briefly, A. hydrophila NJ-35 and C. violaceum CV026 were, respectively, inoculated into fresh LB broth to achieve a concentration of 1 × 10 6 CFU/mL, and we then administered 90 µL strains to a 96-well plate, respectively.…”

Section: Methodsmentioning

confidence: 99%

Carvacrol Inhibits Quorum Sensing in Opportunistic Bacterium Aeromonas hydrophila

Wang

Qin

et al. 2023

Microorganisms

View full text Add to dashboard Cite

Bacterial quorum sensing (QS) plays a crucial role in chemical communication between bacteria involving autoinducers and receptors and controls the production of virulence factors in bacteria. Therefore, reducing the concentration of signaling molecules in QS is an effective strategy for mitigating the virulence of pathogenic bacteria. In this study, we demonstrated that carvacrol at 15.625 μg/mL (1/4 MIC), a natural compound found in plants, exhibits potent inhibitory activity against QS in Chromobacterium violaceum, as evidenced by a significant reduction (62.46%) in violacein production. Based on its impressive performance, carvacrol was employed as a natural QS inhibitor to suppress the pathogenicity of Aeromonas hydrophila NJ-35. This study revealed a significant reduction (36.01%) in the concentration of N-acyl-homoserine lactones (AHLs), a QS signal molecular secreted by A. hydrophila NJ-35, after 1/4 MIC carvacrol treatment. Moreover, carvacrol was found to down-regulate the expression of ahyR/I, two key genes in the QS system, which further inhibited the QS system of A. hydrophila NJ-35. Finally, based on the above results and molecular docking, we proposed that carvacrol alleviate the pathogenicity of A. hydrophila NJ-35 through QS inhibition. These results suggest that carvacrol could serve as a potential strategy for reducing the virulence of pathogenic bacteria and minimizing the reliance on antibiotics in aquaculture.

show abstract

Section: Methodsmentioning

confidence: 99%

Carvacrol Inhibits Quorum Sensing in Opportunistic Bacterium Aeromonas hydrophila

Wang

Qin

et al. 2023

Microorganisms

View full text Add to dashboard Cite

show abstract

“…When the CFS of Lb. rhamnosus GG was incubated with P. aeruginosa PAO1, there was a decrease in AHL activity and levels [ 30 ]. In this study, the authors state that the CFS has inhibited AHL production, potentially by reducing the expression of the AHL-producing gene ( lasI ), albeit this was not evaluated.…”

Section: Mechanism Of Qs Inhibitionmentioning

confidence: 99%

Anti-Quorum Sensing Activity of Probiotics: The Mechanism and Role in Food and Gut Health

2023

View full text Add to dashboard Cite

Background: Quorum sensing (QS) is a cell-to-cell communication mechanism that occurs between inter- and intra-bacterial species and is regulated by signaling molecules called autoinducers (AIs). It has been suggested that probiotics can exert a QS inhibitory effect through their metabolites. Purpose: To provide an overview of (1) the anti-QS activity of probiotics and its mechanism against foodborne pathogenic and spoilage bacteria; (2) the potential role of the QS of probiotics in gut health; and (3) the impact of microencapsulation on QS. Results: Lactobacillus species have been extensively studied for their anti-QS activity and have been found to effectively disrupt QS in vitro. However, their effectiveness in a food matrix is yet to be determined as they interfere with the AI receptor or its synthesis. QS plays an important role in both the biofilm formation of probiotics and pathogenic bacteria. Moreover, in vitro and animal studies have shown that QS molecules can modulate cytokine responses and gut dysbiosis and maintain intestinal barrier function. In this scenario, microencapsulation was found to enhance AI activity. However, its impact on the anti-QS activity of probiotics and its underlying mechanism remains unclear. Conclusions: Probiotics are potential candidates to block QS activity in foodborne pathogenic and food spoilage bacteria. Microencapsulation increases QS efficacy. However, more research is still needed for the identification of the QS inhibitory metabolites from probiotics and for the elucidation of the anti-QS mechanism of probiotics (microcapsules and free cells) in food and the human gut.

show abstract

Optimization of cultural conditions for enhancement of anti-quorum sensing potential in the probiotic strain Lactobacillus rhamnosus GG against Pseudomonas aeruginosa

Cited by 2 publications

References 73 publications

Carvacrol Inhibits Quorum Sensing in Opportunistic Bacterium Aeromonas hydrophila

Carvacrol Inhibits Quorum Sensing in Opportunistic Bacterium Aeromonas hydrophila

Anti-Quorum Sensing Activity of Probiotics: The Mechanism and Role in Food and Gut Health

Contact Info

Product

Resources

About