Optimization of Culture Conditions for Production of Bioactive Metabolites by <i>Streptomyces</i> spp. Isolated from Soil

Abstract

The current work was carried out under a screening program targeted at isolation of bioactive Streptomyces species from soil samples. A total of 54 Streptomyces species were isolated from soil samples, out of which 4 isolates were found to be promising. These isolates were identified as Streptomyces spectabilis, Streptomyces purpurascens, Streptomyces coeruleorubidus and Streptomyces lavendofoliae and their sequences have been deposited in the GenBank. The influence of culture conditions including, incubation time, incubation temperature, initial pH and different carbon and nitrogen sources on growth and bioactive compound formation was investigated. Isolate R1, identified as Streptomyces spectabilis, showed maximum bioactive metabolite production with cellobiose and peptone as the carbon and nitrogen sources, on the 5 th day at pH 5 at 30˚C. The optimum conditions for production by isolate R3, identified as Streptomyces purpurascens, were observed to be starch and casein as the carbon and nitrogen sources, pH 7, temperature 30˚C and an incubation period of eight days. For isolate R5, identified as Streptomyces coeruleorubidus, maximal production resulted on the sixth day at pH 6 and temperature of 35˚C with mannitol and JBM. Isolate Y8, identified as Streptomyces lavendofoliae, was found to produce high levels of bioactive metabolites in the medium supplemented with starch and peptone on the 10 th day at pH 7 and at an incubation temperature of 30˚C. The four strains tested here behaved differently, each one requiring specific conditions for maximum growth as well as bioactive metabolite production.

Antiviral Activity of Secondary Metabolites Produced by Streptomyces Species Isolated From Egyptian Soil.

“…Our result agreed with other researches including Bundale, S. et al, (2015) who developed an efficient fermentation process for the production of secondary metabolites from Streptomyces species by adjusting several cultivation parameters like pH, incubation period and temperature (Bundale et al, 2015).…”

Biological Significance of Marine Actinobacteria of East Coast of Andhra Pradesh, India

“…Sengupta et al (2015) also recorded that most of the actinobacterial strains isolated from Sundarbans mangrove ecosystem preferred polysaccharides (D-galactose and D-raffinose) over monosaccharides for antibiotic production. Sunita et al (2015) observed maximum yields of antimicrobial metabolites by Streptomyces spp. in starch enriched medium.…”

Streptomyces sp. BV410 isolate from chamomile rhizosphere soil efficiently produces staurosporine with antifungal and antiangiogenic properties

“…With Streptomycetes, this has been achieved using classical strain improvement, optimization of process parameters as well as genetic engineering (Kieser et al, 2000;Olmos et al, 2013). The influence of the nutritional parameters on the production of antibiotics is undisputable (Bundale, Begde, Nashikkar, Kadam, & Upadhyay, 2015;Görke & Stülke, 2008). Through the initial optimization of staurosporine production, isolate BV410 was shown to produce 56.25 mg/L of staurosporine ( Figure 3).…”