Optimization of Culture Conditions for Some Identified Fungal Species and Stability Profile ofα-Galactosidase Produced

Abstract

Microbial α-galactosidase preparations have implications in medicine and in the modification of various agricultural products as well. In this paper, four isolated fungal strains such as AL-3, WF-3, WP-4 and CL-4 from rhizospheric soil identified as Penicillium glabrum (AL-3), Trichoderma evansii (WF-3), Lasiodiplodia theobromae (WP-4) and Penicillium flavus (CL-4) based on their morphology and microscopic examinations, are screened for their potential towards α-galactosidases production. The culture conditions have been optimized and supplemented with specific carbon substrates (1%, w/v) by using galactose-containing polysaccharides like guar gum (GG), soya casein (SC) and wheat straw (WS). All strains significantly released galactose from GG, showing maximum production of enzyme at 7th day of incubation in rotary shaker (120 rpm) that is 190.3, 174.5, 93.9 and 28.8 U/mL, respectively, followed by SC and WS. The enzyme activity was stable up to 7days at −20°C, then after it declines. This investigation reveals that AL-3 show optimum enzyme activity in guar gum media, whereas WF-3 exhibited greater enzyme stability. Results indicated that the secretion of proteins, enzyme and the stability of enzyme activity varied not only from one strain to another but also differed in their preferences of utilization of different substrates.

Extracellularα-Galactosidase fromTrichodermasp. (WF-3): Optimization of Enzyme Production and Biochemical Characterization

“…The results from the present paper indicated that Trichoderma sp. exhibited tremendous potential to secrete α -galactosidases extracellularly under certain optimized culture conditions [ 16 ]. Trichoderma sp.…”

“…Selected fungi, that is, Trichoderma sp. (WF-3), were isolated from rhizospheric soil of Phyllanthus emblica (aanwla) collected from the local garden of Sagar, India, as discussed in previous paper [ 16 ]. They were isolated by direct soil plate method [ 17 ] and identified according to [ 18 – 20 ].…”

“…Earlier reports from this laboratory have indicated that soybean flour was the best carbon substrate in secondary media for α -galactosidase production by Trichoderma sp. , which was possibly due to the presence of various suitable nutrients in soybean flour and/or due to its most suitable particle size and consistency required for anchorage, colonization, and enzyme secretion [ 16 ]. The biophysical and biochemical properties of this enzyme have also been determined here under different experimental conditions.…”

A Novel Promising Strain ofTrichoderma evansii(WF-3) for Extracellularα-Galactosidase Production by Utilizing Different Carbon Sources under Optimized Culture Conditions

“…Selected fungi, that is, Trichoderma evansii (WF-3), were isolated from rhizospheric soil of Phyllanthus emblica (aamla) collected from the local garden of Sagar, India [ 33 ]. They were isolated by direct soil plate method [ 34 ] and identified accordingly [ 35 – 37 ].…”

Features of α-Galactosidase Production by Penicillium restrictum

“…Дослідження умов культивування продуцентів біотехнологічно важливих ензимів є запорукою ефективності процесів секреції та отримання кінцевого продукту [1]. Вже давно і успішно для отримання препаратів ензимів різної специфічності використовують метод глибинного культивування мікроорганізмів [2][3][4]. В такий спосіб отримують як позаклітинні, так і внутрішньоклітинні ензими.…”