Optimization of Culture Conditions for Total Carotenoid Amount  Using Response Surface Methodology in Green Microalgae / Ankistrodesmus convolutus

Şener, Neslihan; Demirel, Zeliha; İmamoğlu, Esra; Dalay, Meltem Conk

doi:10.26650/ase2020785091

Cited by 7 publications

(3 citation statements)

References 16 publications

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“…Ankistrodesmus sp. convolutes grew in nitrogen and light stress were found to produce violaxanthin and astaxanthin, according to yet another investigation [43].…”

Section: Carotenoid Profiling By Uplc-q-tof-msmentioning

confidence: 94%

Combinatorial Effect of Multiple Variables on Carotenoids and Lipids Up-Regulation in <em>Monoraphidium</em> sp. for Pharmacological and Nutraceutical Applications

Yadav¹,

Kumar²,

Nikalje³

et al. 2023

Preprint

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Carotenoids are essential pigments that play a vital role in the photosynthetic process of algae and are important for human health and nutrition. Despite their potential value, algal carotenoids are being neglected by biofuel industries. Recent research is aimed at producing microalgal lipids and carotenoids simultaneously under abiotic stresses. In this study, the effect of different abiotic stress factors such as Nitrogen (N), Potassium (K), Phosphorous (P), Sulphur (S), light intensity, and photoperiod on biomass, lipid, and carotenoids synthesis in Monoraphidium sp. (MP) were explored utilizing a statistical method. In addition, 2, 7-dichlorofluorescein diacetate (DCF-DA) mediated spectrofluorimetry was employed to detect the ele-vation in ROS level. The results revealed that variations in nutrients and light con-ditions are the key factors in the up-regulation of biomolecules lipids and carote-noids in the algae. The carotenoids profiling was done by Ultra Performance Liquid Chromatography Quadrupole Time-of-Flight Mass Spectroscopy (UPLC-Q-ToF-MS). Among consisting carotenoids violaxanthin, astaxanthin, and beta-carotene were enhanced by 1.3 folds, 1.19 folds, 1.08 folds, while lutein was declined by 1.32 folds under stressed condition compared to control. The identified carotenoids are rec-ognized for their importance in the pharmaceutical and nutraceutical industries, highlighting the significant role of MP in these sectors.

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“…Ankistrodesmus sp. convolutes grew in nitrogen and light stress were found to produce violaxanthin and astaxanthin, according to yet another investigation [43].…”

Section: Carotenoid Profiling By Uplc-q-tof-msmentioning

confidence: 94%

Combinatorial Effect of Multiple Variables on Carotenoids and Lipids Up-Regulation in <em>Monoraphidium</em> sp. for Pharmacological and Nutraceutical Applications

Yadav¹,

Kumar²,

Nikalje³

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Ankistrodesmus sp. has also shown an increased production of violaxanthin and astaxanthin when grown under nitrogen and light stress [43]. Additionally, the Bracteacoccus aggregatus BM5/15 strain produced astaxanthin and beta-carotene when grown in glass bubble-column photobioreactors [44].…”

Section: Carotenoid Profiling By Uplc-q-tof-msmentioning

confidence: 99%

Combinatorial Effect of Multiple Abiotic Factors on Up-Regulation of Carotenoids and Lipids in Monoraphidium sp. for Pharmacological and Nutraceutical Applications

et al. 2023

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Carotenoids have attracted significant interest due to their potential use in human health and nutrition, and their global demand increases by 3.7% annually. Currently, synthetic carotenoids dominate the market, but possess challenges such as low antioxidant ability, issues with health benefits, and safety concerns. Microalgae are potential producers of natural carotenoids with extraordinary antioxidant properties, although the yield is often low in their natural cycle. The present investigation aimed to analyze the effect of multiple abiotic factors on enhancing algal carotenoids synthesis and other metabolites without affecting growth. The potential microalgae Monoraphidium sp. were grown under altered nutrient and light conditions employing RSM-CCD. The optimized conditions, such as Nitrogen (5 g·L−1), Phosphorus +Potassium (250 mg·L−1), Sulphur (70 mg·L−1), and light (137.5 µmol·m−2·S−1), resulted in increased biomass (1357.36 mg·L−1), lipid accumulation (40.28% of dry biomass), and carotenoids (16.26 µg·mL−1) as compared to the control conditions. The total carotenoids fraction consisted of astaxanthin (14.8%), violaxanthin (3.61%), lutein, (45.12%), 9-cis-β carotene (7.62%), and β-carotene-5,6-epoxide (24.21%). Among them, violaxanthin (1.32-fold), astaxanthin (1.19-fold), 9-cis- β carotene (1.07-fold), and β-carotene-5,6-epoxide (1.08-fold) content increased while lutein (1.32-fold) content decreased significantly. The improvement in algal carotenoids under novel culture conditions provides a significant advantage to pharmaceutical and nutraceutical industries.

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“…Diatom densities were measured every two days for 16 days using UV-Vis spectrophotometry at 600 nm. The observed maximal growth rate (µ) was calculated using Equation 1 (Sener et al, 2022). Based on the data for each experiment which contained three analytical replicates, the mean values with standard deviations were calculated.…”

Section: Diatom and Growth Conditionsmentioning

confidence: 99%

Effect of different abiotic conditions on biomass and fucoxanthin content of Amphora capitellata

Demirel

Erdoğan²,

Karataş

et al. 2022

Frontiers in Life Sciences and Related Technologies

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The intention of the study was to investigate the effect of physical conditions such as aeration rate (1, 3, 5 L/min) as well as chemical conditions including sodium nitrite (NaNO2), urea (CH4N2O) and ammonium chloride (NH4Cl) on the biomass productivity and fucoxanthin concentration of A. capitellata. The optimum cultures were cultivated in f/2 medium using sodium nitrate (NaNO3) at the light intensity of 100 µmol photons/ m2s with aeration rate of 2 L/min in 2 L bubbling bottle photobioreactors. These were then incubated at 22.0±2 °C, under the light intensities of 300 µmol photons/ m2s with three different airflow rates of 1, 3, 5 L/min for 16 days. And then, culture medium was prepared with three different nitrogen sources to achieve higher biomass productivity. During the production of A. capitellata, the maximum specific growth rate of 0.166 day-1, which corresponded to the doubling time 4.166 day, was obtained at the light intensity of 300 µmol photons/ m2s with aeration rate of 1 L/min when sodium nitrate was used. Chlorophyll-a and fucoxanthin contents were also at the highest level in the same light intensity. Dry biomass amount reached the maximum level of 0.66±0.17 g/L in case of NaNO2. In this study, it was identified that the aeration rate of 1 L/min, the light intensity of 300 µmol photons/m2s and sodium nitrate (NaNO3) were the optimum value for the growth of A. capitellata cells and production of biomass and fucoxanthin concentration.

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Optimization of Culture Conditions for Total Carotenoid Amount Using Response Surface Methodology in Green Microalgae / Ankistrodesmus convolutus

Cited by 7 publications

References 16 publications

Combinatorial Effect of Multiple Variables on Carotenoids and Lipids Up-Regulation in <em>Monoraphidium</em> sp. for Pharmacological and Nutraceutical Applications

Combinatorial Effect of Multiple Variables on Carotenoids and Lipids Up-Regulation in <em>Monoraphidium</em> sp. for Pharmacological and Nutraceutical Applications

Combinatorial Effect of Multiple Abiotic Factors on Up-Regulation of Carotenoids and Lipids in Monoraphidium sp. for Pharmacological and Nutraceutical Applications

Effect of different abiotic conditions on biomass and fucoxanthin content of Amphora capitellata

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