Optimization of culture conditions for the production of antimicrobial substances by probiotic Lactobacillus paracasei subsp. Tolerans FX-6

Miao, Jianyin; Xu, Mingbin; Guo, Haoxian; He, Lili; Gao, Xiangyang; DiMarco-Crook, Christina; Xiao, Hang; Cao, Yong

doi:10.1016/j.jff.2015.07.011

Cited by 19 publications

(6 citation statements)

References 27 publications

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“…tolerans (basonym Lactobacillus paracasei subsp. tolerans) FX-6 in Tibetan kefir, has shown potent anti-microbial activity against a broad spectrum of Gram-positive and Gram-negative bacteria and fungi [173]. In the later studies, the actual anti-microbial mechanisms of peptide F1 on Escherichia coli and Staphylococcus aureus were demonstrated necessary to improve its application in food preservation.…”

Section: Future Prospect and Limitationsmentioning

confidence: 99%

Kefir and Its Biological Activities

Azizi

Kumar

Yeap

et al. 2021

Foods

131

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Kefir is a fermented beverage with renowned probiotics that coexist in symbiotic association with other microorganisms in kefir grains. This beverage consumption is associated with a wide array of nutraceutical benefits, including anti-inflammatory, anti-oxidative, anti-cancer, anti-microbial, anti-diabetic, anti-hypertensive, and anti-hypercholesterolemic effects. Moreover, kefir can be adapted into different substrates which allow the production of new functional beverages to provide product diversification. Being safe and inexpensive, there is an immense global interest in kefir’s nutritional potential. Due to their promising benefits, kefir and kefir-like products have a great prospect for commercialization. This manuscript reviews the therapeutic aspects of kefir to date, and potential applications of kefir products in the health and food industries, along with the limitations. The literature reviewed here demonstrates that there is a growing demand for kefir as a functional food owing to a number of health-promoting properties.

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Section: Future Prospect and Limitationsmentioning

confidence: 99%

Kefir and Its Biological Activities

Azizi

Kumar

Yeap

et al. 2021

Foods

131

View full text Add to dashboard Cite

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“…In this study, three key variables with five concentration levels were adopted (given in Table 4). The results of CCD were used to fit a secondorder polynomial [20,21]. The general form of the second-order polynomial Equation (2) was…”

Section: Response Surface Methodology Designmentioning

confidence: 99%

“…The results of ANOVA are shown in Table 5. The model had a very low P value (P < 0.0001), which implied that the model fitted the experimental data very significantly [20,21]. The fitness of the model was examined by the determination coefficient (R 2 = 0.9578), which implied that the sample variation of more than 95% was attributed to the variables and only less than 5% of the total variance could not be explained by the model (Table 5) [30,33].…”

Section: Optimization By Response Surface Methodologymentioning

confidence: 99%

“…However, up till now, there has been no report about M. endolithica's ability to produce antimicrobial metabolites. Orthogonal design, artificial neural networks and response surface methodology (RSM) are common fermentation medium optimization methods, among which RSM has been widely applied in food, pharmaceutical, bioengineering, agriculture, natural product extraction and other fields [20][21][22][23][24][25][26]. In the present study, we combined single factor experiment, Plackett-Burman experiment, steepest ascent experiment and RSM to optimize the medium components for the production of high content antimicrobial metabolites from Micromonospora Y15.…”

Section: Introductionmentioning

confidence: 99%

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Application of response surface methodology to optimize the production of antimicrobial metabolites by Micromonospora Y15

Wang

Zhang

et al. 2017

Biotechnology & Biotechnological Equipment

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The fermentation medium of Micromonospora Y15 was optimized to enhance the production of antimicrobial metabolites with the antimicrobial activity tested against Listeria monocytogenes. First, the optimal medium components (carbon source, nitrogen source and inorganic salt) were determined by the single factor experiment. Second, the Plackett-Burman experiment was used to screen significant factors. Third, the steepest ascent experiment was undertaken to figure out the optimal region of the significant factors. Finally, the central composite experiment was conducted to optimize the final medium components. The single factor experiment results suggested that the optimal medium components were soluble starch, beef extract, peptone, NaCl, K 2 HPO 4 , MgSO 4 , and FeSO 4 , while the Plackett-Burman experiment demonstrated that beef extract, K 2 HPO 4 and soluble starch were significant factors affecting antimicrobial activity. The steepest ascent experiment results further showed the central point of three significant factors were 1.00 g/L of K 2 HPO 4 , 25.00 g/L of soluble starch and 18.00 g/L of beef extract, respectively. Response surface analysis revealed that the optimum values of the tested significant variables for the production of antimicrobial metabolites were 1.03 g/L of K 2 HPO 4 , 27.67 g/L of soluble starch and 18.30 g/L of beef extract, accordingly. Under this optimal condition, the antimicrobial activity of the fermented medium was 1236.79 § 14.56 AU/mL, which increased 3.86 times compared with the initial medium. We thus concluded that the medium composition optimized in the study would be helpful for the production of antimicrobial metabolites by Micromonospora Y15.

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“…According to Fortune Business Insight [4], the global market for probiotics was estimated at US$ 42.55 billion in 2017 and is projected to reach US$ 74.69 billion by 2025. Consumption of probiotics stimulates the growth of beneficial microorganisms in the intestine, thus eliminating potentially harmful bacteria, reinforcing the immune system, and helping in the management of lactose intolerance [5][6][7]. However, to prolong the beneficial effects, probiotic bacteria need to be metabolically stable and active during product consumption and need to remain alive while passing through the host gastrointestinal tract, which is a hostile environment for bacterial survival due to its low pH and presence of biliary salts [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Effect of co-encapsulation using a calcium alginate matrix and fructooligosaccharides with gelatin coating on the survival of Lactobacillus paracasei cells

et al. 2021

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The demand for functional foods is increasing each year because consumers are gaining awareness about the importance of a healthy diet in the proper functioning of the body. Probiotics are among the most commonly known, commercialized, and studied foods. However, the loss of viability of probiotic products is observed during their formulation, processing, and storage. This study aimed to investigate the co-encapsulation of two Lactobacillus paracasei probiotic strains (LBC81 and ELBAL) with fructooligosaccharides (FOS) in a calcium alginate matrix using extrusion technology with gelatin as a coating material. The viability of the strains under gastrointestinal conditions and in storage at low temperature was also assessed. An immobilization yield of more than 59% was observed for both bacterial strains. Exposure to 2% biliary salts led to a decrease in the viability of free cells in the two L. paracasei strains, whereas the viability of microencapsulated cells increased up to 47%. After 35 days of storage at 4°C, the population of free cells was reduced, but microencapsulated cells remained stable after storage at low temperature. LBC81 bacteria microencapsulated with 1.5% FOS coated with gelatin were the most resistant to the stressful environments tested. Therefore, these results showed that co-encapsulation with FOS in a calcium alginate matrix coated with gelatin improved L. paracasei survival and may be useful for the development of more resistant probiotics and new functional foods.

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Optimization of culture conditions for the production of antimicrobial substances by probiotic Lactobacillus paracasei subsp. Tolerans FX-6

Cited by 19 publications

References 27 publications

Kefir and Its Biological Activities

Kefir and Its Biological Activities

Application of response surface methodology to optimize the production of antimicrobial metabolites by Micromonospora Y15

Effect of co-encapsulation using a calcium alginate matrix and fructooligosaccharides with gelatin coating on the survival of Lactobacillus paracasei cells

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