Optimization of Culture Conditions of<i>Brevibacterium SP.</i>A4 for the Production of Nitrile Hydratase

Bernet, Nicolas; Arnaud, A.; Galzy, P.

doi:10.3109/10242429008992069

Cited by 14 publications

(8 citation statements)

References 26 publications

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“…Sonicated cells of strains R312, M2, and Ad had the same activities on adiparnide. This result confirmed the results obtained by Bernet (1989). Strain Ad had a modified cell wall.…”

Section: Isolation Of Strain Acv2supporting

confidence: 95%

“…In contrast t o these activity measurements with whole cells, assays using the El enzyme extracts and the protoplasts did not show any difference between the Ad and the wild-type strains (Bernet 1989).…”

Section: Isolation Of Strain Admentioning

confidence: 58%

“…This Brevibacterium sp. R3129 M2, and result is logical because strain ACV2 was derived from A CV2 amidase activities Bernet (1989) has shown that Brevibacterium sp. Ad was Brevibacterium sp.…”

Section: Isolation Of Strain Acv2mentioning

confidence: 98%

“…L -' MgSO,.H,O, 0.01 g.L-' FeS0,.7HZO, 0.002 g.L-' thiamine chlorhydrate) described by Bernet et al (1990) and to which were added glucose (10 g . L -I) and ammonium chloride (5 g. L -I); medium 2, basic MMT medium to which were added glucose (10 g.L-I) and adipamide (1 g.L-I); medium 3, medium 2 to which were added thioacetamide (80 mM) and N-methylacetamide (250 mM); medium 4, MMT medium to which were added glucose (10 g. L-') and cyano-5-valeric acid (1 g .~-' ) .…”

Section: Media and Culture Conditionsmentioning

confidence: 99%

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Isolation of Brevibacterium sp. R312 mutants potentially useful for the enzymatic production of adipic acid

Moreau¹,

Bernet²,

Arnaud³

et al. 1993

Can. J. Microbiol.

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P. 1993. Isolation of Brevibacterium sp. R312 mutants potentially useful for the enzymatic production of adipic acid. Can. J. Mutants of Brevibacterium sp. R312 were isolated for the production of adipic acid from adiponitrile. One mutant (Ad) with a modified cell wall showed activity against adipamide 3 times greater than the wild type. Another mutant (ACV2) derived from the Ad strain had 30 times more activity on cyano-5-valeric acid, and 7 times more on adipamide, than the wild type. The presence of an amidase acting on amide intermediates in the hydrolysis of dinitriles to organic acids was demonstrated in these mutants.Key words: Brevibacterium sp., adiponitrile, cyano-5-valeric acid, adipamide, adipic acid, mutant.MOREAU, J. L., BERNET, N., ARNAUD, A., et GALZY, P. 1993. Isolation of Brevibacterium sp. R312 mutants potentially useful for the enzymatic production of adipic acid. Can. J. Microbiol. 39 : 524-528.Des mutants de Brevibacterium sp. R312 ont Ct C isoles en vue de la production d'acide adipique a partir d'adiponitrile.Un mutant a la paroi modifiCe (Ad) prCsente une activitk sur I'adipamide environ 3 fois plus importante que la souche sauvage. Un autre mutant dCrivC de Ad, ACV2, possede une activitC 30 fois plus importante sur l'acide cyano-5-valkrique et 7 fois plus importante sur l'adipamide que la souche sauvage. Une nouvelle amidase responsable de la dCgradation des amides intervenant dans I'hydrolyse des dinitriles en acides organiques a Ct C mise en Cvidence chez ces mutants. Mots clks : Brevibacterium sp., adiponitrile, acide cyano-5-valkrique, adipamide, acide adipique, mutant.

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“…Sonicated cells of strains R312, M2, and Ad had the same activities on adiparnide. This result confirmed the results obtained by Bernet (1989). Strain Ad had a modified cell wall.…”

Section: Isolation Of Strain Acv2supporting

confidence: 95%

Section: Isolation Of Strain Admentioning

confidence: 58%

Section: Isolation Of Strain Acv2mentioning

confidence: 98%

Section: Media and Culture Conditionsmentioning

confidence: 99%

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Isolation of Brevibacterium sp. R312 mutants potentially useful for the enzymatic production of adipic acid

Moreau¹,

Bernet²,

Arnaud³

et al. 1993

Can. J. Microbiol.

Self Cite

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“…A4 [29] (formerly Brevibacterium sp. R312 strain A4, [30] Technische Universiteit Delft, LMD#79.2) in 890 mL of deionized water at 5 8C were added 100 g (0.99 mol) of 3-HVN with stirring.…”

Section: Preparation Of 3-hydroxyvaleramide (3-hvam)mentioning

confidence: 99%

Biocatalytic Hydrolysis of 3‐Hydroxyalkanenitriles to 3‐Hydroxyalkanoic Acids

et al. 2003

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Microbial catalysts having a combination of nitrile hydratase and amidase activities had a significantly-higher specific activity for hydrolysis of 3-hydroxyalkanenitriles than microbial nitrilase catalysts. Comamonas testosteroni 22 ± 1, Dietzia sp. ADL1 and Comamonas testosteroni 5-MGAM-4D nitrile hydratase/amidase biocatalysts each hydrolyzed 3-hydroxyvaleronitrile to 3-hydroxyvaleric acid (as the ammonium salt) in 99 ± 100% yields, but in consecutive batch reactions with catalyst recycle, alginate-immobilized C. testosteroni 5-MGAM-4D had superior enzyme stability and volumetric productivity. In a series of 85 consecutive batch reactions with biocatalyst recycle for the production of 1.0 M 3-hydroxyvaleric acid, the recovered nitrile hydratase and amidase activities in the final reaction were 29% and 40%, respectively, of the initial activities. The catalyst productivity for this series of reactions was 670 g 3-hydroxyvaleric acid/g dry cell weight (50 g 3-hydroxyvaleric acid/g biocatalyst bead), and the volumetric productivity of the initial reaction in the series was 44 g 3-HVA/L/h. Similar results were obtained with alginate-immobilized C. testosteroni 5-MGAM-4D for the hydrolysis of 3-hydroxybutryonitrile and 3-hydroxypropionitrile to the corresponding 3-hydroxyalkanoic acid ammonium salts.

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Hydrolysis of Nitriles to Amides

Yanenko

Oßwald

2012

Enzyme Catalysis in Organic Synthesis

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Optimization of Culture Conditions ofBrevibacterium SP.A4 for the Production of Nitrile Hydratase

Cited by 14 publications

References 26 publications

Isolation of Brevibacterium sp. R312 mutants potentially useful for the enzymatic production of adipic acid

Isolation of Brevibacterium sp. R312 mutants potentially useful for the enzymatic production of adipic acid

Biocatalytic Hydrolysis of 3‐Hydroxyalkanenitriles to 3‐Hydroxyalkanoic Acids

Hydrolysis of Nitriles to Amides

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