Optimization of Culture Conditions Using One-Factor-at-Time Methodology and Partial Purification of Amylase from Aspergillus niger of DTO: H5 under Solid State Fermentation

Ire, Francis Sopuruchukwu; Eruteya, O. C.; Amaechi, V.

doi:10.20546/ijcmas.2017.605.035

Cited by 3 publications

(4 citation statements)

References 36 publications

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“…The result of this study also differed with the findings of Gupta et al, (2008) who reported an incubation period of 5 days for optimum amylase production by A. niger. The maximum amylase production which occurred after 96 h (4 days) is also not in agreement with the findings of Ire et al (2017) who reported that amylase production by A. niger DTO:H15 occurred after 48 h of incubation at pH at initial pH 6.0 under solid state fermentation. Our result also in contrast with the findings of Negussie et al, (2019) who reported that maximum amylase production by Penicillium sp was obtained at 72 h of incubation.…”

Section: Discussioncontrasting

confidence: 64%

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Isolation, Screening and Time Course Study of Amylase-producing Fungi from Garri Processing Environment

Ire

Wokoma

Okoli

2020

JLBSR

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Amylases (E.C.3.2.1.1) are enzymes which catalyze the breakdown of -1,4 glycosidic linkages of starch to simple sugars and different monomeric products. Microbial amylases are essential enzymes compared with animals and plants amylases in industrial applications. Thus, in southern Nigeria, garri processing waste-water from mills poses a serious environmental challenge and this situation could be exploited by utilizing microorganisms colonizing the area to manufacture microbial products. This present study was aimed at evaluating the potential of fungi isolated from garri processing environment for amylase secretion and conduct a time course study of the enzyme production. Fungi were isolated from the soil and waste water from garri processing sites using Standard Microbiological Procedures. The five fungi isolated from garri processing environmental samples were screened using Lugols iodine. Three fungal isolates were then selected on the hydrolysis of starch in qualitative zonation agar plates but one isolate named B among them was better in starch hydrolysis related to highest clear zone plates. After qualitative screening, the three hyper production amylase extracted fungi were identified according to the morphological characteristics. The hyper producing amylase isolate B was then identified as Aspergillus flavus (RCBBR_AEAFUN2) and recorded as a novel strain in southern Nigeria according to molecular characteristics, which was selected for further studies. The current results of time course study showed that the maximum growth (2.453 g) occurred after 24 h of incubation at pH 4.5 while maximum enzyme production (2.3 U/ml/min) was obtained after 96 h of incubation at pH 3.5. Therefore, the hyper producing amylase from garri processing sites, Aspergillus flavus (RCBBR_AEAFUN2) novel strain has great prospects for future biotechnological and industrial applications and help in ameliorating the environmental pollution posed by the waste-water.

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Section: Discussioncontrasting

confidence: 64%

“…Inoculum preparation was performed as previously reported (Ire et al, 2017). Fungal cells from 48 h old slant culture was used for the inoculation.…”

Section: E Inoculum Preparationmentioning

confidence: 99%

Isolation, Screening and Time Course Study of Amylase-producing Fungi from Garri Processing Environment

Ire

Wokoma

Okoli

2020

JLBSR

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“…The result of this research has shown that Chinese rice bran is the best substrate for amylase enzyme production and, thus is a suitable nutrient source for amylase production by microorganisms. The result of this research is not in agreement with the work of Ire et al who reported a maximum amylase activity of 305.26±0.00UI/mL using wheat bran as substrate [21]. It is also not in line with the work of Andi et al, [22] who used A. niger with different variety of waste substrates, i.e.…”

Section: Discussioncontrasting

confidence: 97%

“…The result of this study has shown that A. niger USGB:1024 can be used for the commercial production of amylase enzyme since it is capable of producing amylase enzyme with highest activity [19,24]. This result indicates variation in amylase production among different strains of A. niger [21,25,26]. It was reported that A. niger isolated from soil have higher amylase production on different agricultural residues [8,15,24,[27][28][29]…”

Section: Discussionmentioning

confidence: 99%

Comparative Analysis of Amylase Enzyme Produced by Aspergillus niger Using Rice and Maize Bran as Substrates

Bukar,

Musa,

Yusuf

et al. 2023

Asian J Plant Biol

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Amylase is used in the degradation of starch-containing materials into its simpler constituents. This study was aimed at comparing amylase production by Aspergillus niger isolated from different sources on different varieties of rice and maize bran. Different strains of A. niger were isolated from different sources and were identified using cultural, morphological and molecular characteristics. A. niger USGB:1024 isolated from soil obtained from a maize mill and A. niger BGSU:17-00030 isolated from spoiled tomato were used for amylase enzyme production as both gave positive results during screening. Amylase enzyme was produced using 15g of substrates, temperature 30 C, pH 6 and inoculum density of 1 ×106. A. niger USGB:1024 was found to produce an amylase enzyme with the highest activity (3.15±0.1 IU) on Chinese rice bran at fermentation day 5. A. niger BGSU:17-00030 also produced amylase enzyme with the highest activity (1.46±0.01 IU) on Chinese rice bran at 5 days of fermentation. The result has shown that A. niger USGB:1024 produced amylase enzyme with the highest activity than A. niger BGSU:17-00030.

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Optimized Solid-State Fermentation Medium Enhances the Multienzymes Production from Penicillium citrinum and Aspergillus clavatus

Shruthi

Achur

2020

Curr Microbiol

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Optimization of Culture Conditions Using One-Factor-at-Time Methodology and Partial Purification of Amylase from Aspergillus niger of DTO: H5 under Solid State Fermentation

Cited by 3 publications

References 36 publications

Isolation, Screening and Time Course Study of Amylase-producing Fungi from Garri Processing Environment

Isolation, Screening and Time Course Study of Amylase-producing Fungi from Garri Processing Environment

Comparative Analysis of Amylase Enzyme Produced by Aspergillus niger Using Rice and Maize Bran as Substrates

Optimized Solid-State Fermentation Medium Enhances the Multienzymes Production from Penicillium citrinum and Aspergillus clavatus

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