Optimization of Culture Media Formulations for Micropropagation of Lepisanthes fruticosa

Rahman, Zuraida Ab; Ali, Mohd Shukuri Mohamad; Ghazalli, Mohd Norfaizal; Awang, Khadijah; Othman, Ayu Nazreena

doi:10.13005/bbra/2607

Cited by 5 publications

(4 citation statements)

References 58 publications

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“…Data analysis for the traits in different culture media (MS, B5, WPM and, DCR) indicated that there was a significant difference between all treatments except shoot length (Table 1). Ab Rahman et al (2018) stated that the semi-solid WPM medium was most effective in shoot initiation and shoot proliferation for micropropagation of Lepisanthes Fruticosa as well. Statistical analysis showed that the effect of culture medium on root length was significant at level 5% (Table 1).…”

Section: Resultsmentioning

confidence: 99%

In vitro micro-tuberization in Bunium persicum with different media and sucrose concentrations

Ouzhand,

Pourseyedi,

Mohayeji

et al. 2023

IJGPB

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Bunium persicum (Boiss.) B. Fedtsch. is one of economically important member of the Apiaceae family with a tuberous root. Production of this plant is restricted due to seed dormancy and long juvenile time. The objective of this study was to determine the best media and optimum concentration of sucrose for in vitro microtuberization of Black Zira. Therefore, different basal media (Murashige and Skoog (MS), Woody Plant Medium (WPM), Gamborg’s B5, and Gupta and Durzan’s DCR) with 3% sucrose were used for microtuber induction. Then best media was tested by varying concentrations of sucrose (3, 6, 9, and 12%) to increase morphological characteristics diameter and weight of microtubers. The experiment was done in a Randomized Complete Block Design with four treatments and 8 replications per treatment. According to the results obtained in this study, the highest mean of the root length (4.5196 cm), tuber length (1.175 cm), tuber width (0.3575 cm), tuber diameter (4.1 mm), and tuber weight (0.1472 g) after six weeks was observed in the WPM medium. Among the four concentrations of sucrose in WPM medium supplemented with 6% sucrose indicated a better response than the other concentrations in terms of microtuber diameter (7.675 mm), and weight (0.2687 g) average and was found optimum. Therefore, the WPM was recognized as the best medium for micro-tuberization.

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Section: Resultsmentioning

confidence: 99%

In vitro micro-tuberization in Bunium persicum with different media and sucrose concentrations

Ouzhand,

Pourseyedi,

Mohayeji

et al. 2023

IJGPB

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“…Benzyl Amino Purine (BAP) is a synthetic growth regulator of the cytokinin class which is often used in in vitro culture [5]. The use of WPM containing 1.0 mg/L BAP showed the best percentage of shoot induction rate on Lepisanthes fruticosa [6]. The use of WPM containing BAP 4 ppm + NAA 0.5 ppm showed the best results in shoot emergence time, leaf emergence time, number of leaves, root The appearance of buds is characterized by the presence of a greenish-colored bumps (multiple-bud) on the surface of the explants (Figure 1 and Figure 2).…”

Section: Introductionmentioning

confidence: 99%

Effect of BAP concentration on the growth of several types of rambutan plants (Nephelium lappaceum L.) in vitro

Yuniastuti,

Nugroho,

Sukaya

et al. 2024

IOP Conf. Ser.: Earth Environ. Sci.

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In vitro seed propagation is needed as an alternative to providing superior rambutan planting material. This research aimed to examine the effect of BAP on the growth of several varieties of rambutan in vitro. The method used was a completely randomized design with 2 factors, the first factor was rambutan varieties (binjai, rapiah, and ace), and the second factor was BAP concentrations (0, 2, 4, and 6 ppm). There were 12 treatment combinations and 3 times replications. Variables observed was analyzed using analysis of variance followed by 5% Duncan’s Multiple Range Test and other data (leaf emergence time, number of leaves, root emergence time, number of roots, and root length) were analyzed descriptively. The results showed that the combination of 0 ppm BAP and binjai variety gave the best results on the shoot length, number of leaves, and number of roots. The 0 ppm BAP treatment gave the best results on the number of shoots, shoot length, number of leaves, number of roots, and root length. Binjai variety treatment gave the best results on the time of shoot emergence, shoot length, time of leaf appearance, number of leaves, time of root appearance, and number of roots.

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“…At the same time most often most of researchers at microcloning of grapes use as basic mineral basis copy-book of the Murasige environment and Skuga (MS) [1][2][3][4][5][6][7][8][9][10]. Less often works in which authors use insignificant modifications of mineral basis of this environment [11] meet, or study against the background of MS efficiency of other standard environments [12][13][14][15][16][17]. In spite of the fact that researchers give preference in the majority of scientific works on microclonal reproduction of plants to the MS environment, this environment possesses number of the shortcomings caused by the fact that it was developed for cultivation of kallusny cells of tobacco and its main shortcoming at regeneration of meristems is high concentration of salts of 4.7 g/l.…”

Section: Introductionmentioning

confidence: 99%

Improvement of the copy-book of nutrient medium for input of meristems of grapes in the culture of in vitro

Rebrov

2020

E3S Web Conf.

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Results of researches on development of substrate for input of meristems of grapes are provided to the culture of in vitro for the purpose of increase in their regenerative ability at improvement from chronic diseases. In the advanced environment in comparison with analogs the general content of macrosalts was reduced and their ratio taking into account consumption and features of their absorption from nutrient solution is optimized by grape plant. During tests of new substrate its efficiency for grapes grades from various environmental-geographical groups was established. Higher and stable percent of survival and regeneration of extremely small meristems, against the background of various modifications of copy-book of traditionally applied substrate of Murasige and Skuga is established.

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Optimization of Culture Media Formulations for Micropropagation of Lepisanthes fruticosa

Cited by 5 publications

References 58 publications

In vitro micro-tuberization in Bunium persicum with different media and sucrose concentrations

In vitro micro-tuberization in Bunium persicum with different media and sucrose concentrations

Effect of BAP concentration on the growth of several types of rambutan plants (Nephelium lappaceum L.) in vitro

Improvement of the copy-book of nutrient medium for input of meristems of grapes in the culture of in vitro

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