Optimization of culture medium for Sanghuangporus vaninii and a study on its therapeutic effects on gout

Guo, Quan; Zhao, Liying; Zhu, Yuhua; Wu, Jiang; Hao, Cuiting; Song, Shuang; Shi, Wei

doi:10.1016/j.biopha.2020.111194

Cited by 20 publications

(24 citation statements)

References 41 publications

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“…However, many studies have mainly focused on the extraction, isolation and component analysis of S. vaninii, and there are few studies regarding its bioactivity [7]. Qiong Guo et al found that S. vaninii exhibits a strong capacity for free radical scavenging, can effectively alleviate cellular oxidative stress and provides therapeutic effects on gout [19]. Xilin Wan et al reported that polysaccharide from S. vaninii have anti-tumour functions through the activation of the p53 signalling pathway in breast cancer MCF-7 cells.…”

Section: Introductionmentioning

confidence: 99%

Component Analysis and Anti-Colorectal Cancer Mechanism via AKT/mTOR Signalling Pathway of Sanghuangporus vaninii Extracts

et al. 2022

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Sanghuangporus vaninii (Ljub.) L.W. Zhou & Y.C. Dai (SV) is a major cultivar of Sanghuang, which is well known as an excellent anti-tumour drug and reaches the mainstream market in China. Water, 60% ethanol and 95% ethanol were used to extract the drug, and three kinds of polar extracts were obtained separately. Compared with water extracts and 95% ethanol extracts, the 60% ethanol extract had the highest flavonoid content, and its polysaccharide content was greater than that in the 95% ethanol extract and lower than that in the water extract. Its essential components were phenolics whose majority were phenolic acids, flavonoids and phenylpropanoids. This extract has better inhibition effects on the proliferation of SW480 human colon cancer cells, inducing cell apoptosis and blocking G2/M period cells. It can significantly inhibit gene expression and reduce the activation of the AKT/mTOR signalling pathway. The anti-cancer activity of the 60% ethanol extract is satisfactory and may be a result of the combined effects of polysaccharides and flavonoids. The data suggest that the 60% ethanol extract can be used as an adjuvant for chemotherapy and as a potential anti-cancer agent with broad development prospects.

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Section: Introductionmentioning

confidence: 99%

Component Analysis and Anti-Colorectal Cancer Mechanism via AKT/mTOR Signalling Pathway of Sanghuangporus vaninii Extracts

et al. 2022

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“…In the present study, the extracts from host of S. vaninii can significantly promote the growth of S. vaninii mycelium in liquid culture. Guo et al (2021) found that the polymer lignin, as a component of branches, can promote the growth of S. vaninii mycelium at the concentration of 0.06 g/l. Findings imply that MBE may have similar effects.…”

Section: Discussionmentioning

confidence: 96%

“…For example, Wu et al (2019) found that hemicellulose and lignin, the major components of wood, can stimulate mycelial growth and polysaccharide biosynthesis in L. edodes . Lignin can promote the growth of S. vaninii mycelia in culture plate at the concentration of 0.06 g/l ( Guo et al, 2021 ). S. vaninii is a wood-inhabiting fungus that parasitises mulberry ( Huo et al, 2020 ) and poplar ( Shen et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Non-targeted metabonomics and transcriptomics revealed the mechanism of mulberry branch extracts promoting the growth of Sanghuangporus vaninii mycelium

Huo

Sun²,

Pan³

et al. 2022

Front. Microbiol.

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Sanghuangprous vaninii is a wood-inhabiting fungus, and its mycelium and fruiting body show excellent medicinal values. Mulberry is one of the major hosts of S. vaninii, however, the mechanism of mulberry affecting the growth of S. vaninii has not been reported. In the present study, a mulberry-inhabiting strain of S. vaninii was selected to explore the effects of mulberry branch extracts (MBE) on the growth of the strain. Results showed that MBE could significantly promote the growth of S. vaninii mycelium at the concentration of 0.2 g/l. After 16 days of liquid culture, the dry weight of mycelium in 0.2 g/l MBE medium was higher by three times compared with that in the control. The non-targeted metabonomic analysis of the culture medium at different culture times and concentrations was conducted to find the key components in MBE that promoted the growth of S. vaninii mycelium. Under the different concentrations of MBE culture for 10 and 16 days, 22 shared differential metabolites were identified. Next, in accordance with the peak value trend of these metabolites, HPLC–MS and liquid culture validation, four components derived from MBE (i.e., scopoletin, kynurenic acid, 3,5-dihydroxybenzoic acid and 2,4-dihydroxybenzoic acid) could significantly increase the growth rate of mycelium at the concentration of 2 mg/l. Transcriptomic and qRT-PCR analyzes showed that MBE could upregulate hydrolase-related genes, such as serine–glycine–asparaginate–histidine (SGNH) hydrolase, alpha-amylase, poly-beta-hydroxybutyrate (PHB) depolymerase, glycosyl hydrolase family 61, cerato-platanin protein and Fet3, which might enhance the nutrient absorption ability of S. vaninii. Importantly, MBE could significantly increase the content of harmine, androstenedione and vesamicol, which have been reported to possess various medicinal effects. Results suggested that MBE could be an excellent additive for liquid culture of S. vaninii mycelium, and these hydrolase-related genes also provided candidate genes for improving the nutrient absorption capacity of S. vaninii.

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“…In addition, it was found that the contents of active compounds and their biological activities in artificial cultivated fruiting bodies of Sanghuangporus were higher and stronger than those in wild ones [ 13 , 14 ]. Therefore, several strategies were applied into the mycelium cultures or fruiting body cultivation to improve the efficiency and quality of Sanghuangporus production, including mutation breeding [ 15 ], the optimization of medium composition [ 16 ], the improvement of cultural condition [ 17 ], and the addition of exogenous fungal elicitor [ 18 ]. It was worth mentioning that the growth and development of both wild and cultivated macrofungal fruiting bodies were in association with various endophytic microorganisms, in which the community structures and roles of bacteria inhabiting in fruiting bodies have been widely explored and reported.…”

Section: Introductionmentioning

confidence: 99%

Community composition and trophic mode diversity of fungi associated with fruiting body of medicinal Sanghuangporus vaninii

Gao

Zhang

et al. 2022

BMC Microbiol

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Background: The microbial symbionts of macrofungal fruiting body have been shown to play momentous roles in host growth, development, and secondary metabolism. Nevertheless, there is no report on the fungal diversity of Sanghuangporus, a medicinal and edible homologous macrofungus as “forest gold”, which has good effects on antioxidation, boosting immunity and curing stomachache. Here, the diversity and functional group of fungi associated with the fruiting body of the most widely applied S. vaninii were characterized by high-throughput sequencing and FUNGuild tool for the first time. Results: Total 11 phyla, 34 classes, 84 orders, 186 families, and 328 genera were identified in the fruiting body, and our results revealed that the fungal community was dominated by the host fungal taxonomy with absolute superiority (more than 70%), namely, Basidiomycota, Agaricomycetes, Hymenochaetales, Hymenochaetaceae, and genus of Phellinus corrected to Sanghuangporus. Simultaneously, the reads allocated into non-host fungal operational taxonomic units were largely dominated by Ascomycota, Sordariomycetes, Sordariales, Mortierellaceae, and Mortierella. Furthermore, the endophytic fungi were assigned into three trophic modes of “saprotroph” (53.2%), “symbiotroph” (32.2%), and “pathotroph” (14.1%), in which the category of “plant pathogen” was highest enriched with relative abundance of 91.8%, indicating that the endophytic fungi may have the potential to adjust the growth and metabolism of host S. vaninii. Conclusion: Altogether, this report firstly provided new findings that can be inspiring for further in-depth studies to exploit bioactive microbial resources for increased production of Sanghuangporus via coculture, as well as to explore the relationship between macrofungi and their associated endophytes.

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Optimization of culture medium for Sanghuangporus vaninii and a study on its therapeutic effects on gout

Cited by 20 publications

References 41 publications

Component Analysis and Anti-Colorectal Cancer Mechanism via AKT/mTOR Signalling Pathway of Sanghuangporus vaninii Extracts

Component Analysis and Anti-Colorectal Cancer Mechanism via AKT/mTOR Signalling Pathway of Sanghuangporus vaninii Extracts

Non-targeted metabonomics and transcriptomics revealed the mechanism of mulberry branch extracts promoting the growth of Sanghuangporus vaninii mycelium

Community composition and trophic mode diversity of fungi associated with fruiting body of medicinal Sanghuangporus vaninii

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