Optimization of Diesel Biodegrading Conditions Using Response Surface Methodology Based on Central Composite Design

Umar, Zubairu Darma; Musa, Aminu; Yunusa, Y. R.

doi:10.1080/10406638.2020.1823859

Cited by 5 publications

(2 citation statements)

References 30 publications

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“…(A35), Alcaligenes aquatilis (A36) and Alcaligenes faecalis UMYU001 (MN519483.1), A38. These isolates were reported in the previous literatures to be able to degrade diesel oil (Lee et al, 2006;Nikhil et al, 2013;Karthika et al, 2014;Rehman et al, 2015;Umar et al, 2020).…”

Section: Discussionmentioning

confidence: 64%

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Survival Response of Consortium Isolates from Diesel Contaminated Soil within Katsina State, Nigeria

2020

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Environmental increase in the spilled diesel creates serious damages to our natural ecosystems. Biodegradation provide the best removal alternative for such diesel contamination. This study was carried out to monitor the bacteria survival response during diesel oil biodegradation. Bacteria isolation was carried out using plating technique and screened at varying diesel concentrations (0 to 10%, v/v) before being identified using the 16S rRNA gene sequencing. Bacteria consortia were formulated using the screened isolates through mathematical permutation approach. Prepared bacteria resting cells of the selected consortium was grown at 2%, v/v diesel oil which was co-contaminated with varying concentrations of Mg++, Mn++, Zn+, Co++ and Fe++ (0 to 4 g/L) in mineral salts medium. Total of 47 different bacteria strains were isolated and the finally screened isolates were identified as Alcaligenes sp., Ochrobactrum sp., Alcaligenes aquatilis and Alcaligenes faecalis UMYU001 (MN519483.1). These bacteria were found to be compatible with one another despite being obtained from different environments. Of the prepared consortia, combination 11 (CST11) was found to produce the highest population of 1.6 x 104 cfu/mL after 48 hours at 2% v/v diesel oil. This CST11 survived optimally at 35°C, 7.0, and 2% v/v of the temperature, pH and diesel oil respectively. Also, the resistance thresholds of the metals for CST11 include Mg++ (3.5 g/L) while Mn++, Zn++, Co++ and Fe++ were only resisted at 1 g/L. This recommends the consortium as good enough in surviving at the sole expense of diesel oil even in the presence of heavy metals co-contaminants.

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Section: Discussionmentioning

confidence: 64%

“…Extreme pH values affect microbes by breaking the DNA and catalyze protein denaturation which is directly related to survival, growth and proliferation of soil microorganisms (Rousk et al, 2009). In a previous studies by Umar et al (2017;2020), the best pH for proliferation of hydrocarbonoclastic bacteria was determined to be neutral.…”

Section: Discussionmentioning

confidence: 99%