Optimization of dilute acid pretreatment of Agave lechuguilla and ethanol production by co-fermentation with Escherichia coli MM160

Díaz-Blanco, Deniss I.; Cruz, Jesús R. de La; López-Linares, Juan Carlos; Morales-Martínez, Thelma K.; Ruiz, Encarnación; Ríos-González, Leopoldo J.; Romero, Inmaculada

doi:10.1016/j.indcrop.2018.01.074

Cited by 46 publications

(21 citation statements)

References 48 publications

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“…This extractive content is high compared to other lignocellulosic biomasses, such as wheat straw [ 35 ], sugarcane bagasse [ 36 ], or Eucalyptus globulus [ 37 ], and is even higher than in other residues from the olive industry, such as olive stones (an extractive content of 10.5; [ 38 ]) or olive tree prunings (25–28%) [ 10 , 39 ]. Nevertheless, the high extractive content of EOP is comparable to other biomasses such as olive leaves [ 10 , 40 ] or Agave lechugilla [ 41 ] with 37% extractive content.…”

Section: Resultsmentioning

confidence: 90%

Valorisation of Exhausted Olive Pomace by an Eco-Friendly Solvent Extraction Process of Natural Antioxidants

et al. 2020

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Exhausted olive pomace (EOP) is the waste generated from the drying and subsequent extraction of residual oil from the olive pomace. In this work, the effect of different aqueous solvents on the recovery of antioxidant compounds from this lignocellulosic biomass was assessed. Water extraction was selected as the best option for recovering bioactive compounds from EOP, and the influence of the main operational parameters involved in the extraction was evaluated by response surface methodology. Aqueous extraction of EOP under optimised conditions (10% solids, 85 ºC, and 90 min) yielded an extract with concentrations (per g EOP) of phenolic compounds and flavonoids of 44.5 mg gallic acid equivalent and 114.9 mg rutin equivalent, respectively. Hydroxytyrosol was identified as the major phenolic compound in EOP aqueous extracts. Moreover, these extracts showed high antioxidant activity, as well as moderate bactericidal action against some food-borne pathogens. In general, these results indicate the great potential of EOP as a source of bioactive compounds, with potential uses in several industrial applications.

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Section: Resultsmentioning

confidence: 90%

Valorisation of Exhausted Olive Pomace by an Eco-Friendly Solvent Extraction Process of Natural Antioxidants

et al. 2020

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“…In this concern, there is a growing interest in the use of A. lechuguilla for the bioenergy industry [ 14 , 15 , 16 , 17 ]. The lechuguilla is also considered for uses in construction [ 18 , 19 ], soil and water decontamination [ 20 , 21 ], and bio-based polymers [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

High Throughput Profiling of Flavonoid Abundance in Agave lechuguilla Residue-Valorizing under Explored Mexican Plant

Morreeuw

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Ríos-González

et al. 2021

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Agave lechuguilla waste biomass (guishe) is an undervalued abundant plant material with natural active compounds such as flavonoids. Hence, the search and conservation of flavonoids through the different productive areas have to be studied to promote the use of this agro-residue for industrial purposes. In this work, we compared the proportion of total flavonoid content (TFC) among the total polyphenolics (TPC) and described the variation of specific flavonoid profiles (HPLC-UV-MS/MS) of guishe from three locations. Descriptive environmental analysis, using remote sensing, was used to understand the phytochemical variability among the productive regions. Furthermore, the effect of extractive solvent (ethanol and methanol) and storage conditions on specific flavonoid recovery were evaluated. The highest TPC (16.46 ± 1.09 GAE/g) was observed in the guishe from region 1, which also had a lower normalized difference water index (NDWI) and lower normalized difference vegetation index (NDVI). In contrast, the TFC was similar in the agro-residue from the three studied areas, suggesting that TFC is not affected by the studied environmental features. The highest TFC was found in the ethanolic extracts (6.32 ± 1.66 QE/g) compared to the methanolic extracts (3.81 ± 1.14 QE/g). Additionally, the highest diversity in flavonoids was found in the ethanolic extract of guishe from region 3, which presented an intermedia NDWI and a lower NDVI. Despite the geo-climatic induced variations of the phytochemical profiles, the results confirm that guishe is a valuable raw material in terms of its flavonoid-enriched bioactive extracts. Additionally, the bioactive flavonoids remain stable when the conditioned agro-residue was hermetically stored at room temperature in the dark for nine months. Finally, the results enabled the establishment of both agro-ecological and biotechnological implications.

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“…As observed, the simultaneous consumption of glucose and XGM was determined in the fermentation of both detoxified hydrolysates, although the consumption rate of XGM sugars by E. coli was also higher for the hydrolysate detoxified with activated charcoal. Simultaneous consumption of glucose and XGM by E. coli has also been reported in the fermentation of hemicellulosic hydrolysates from Agave lechuguilla [31], corncobs [33], and rapeseed straw [13]. It is worth mentioning that levels of arabinose identified in the EOP hydrolysates were not relevant (<9% of total sugars).…”

Section: Ethanol Fermentation By E Colimentioning

confidence: 75%

“…Activated charcoal treatment is a commonly used detoxification method. The excellent capacity of activated charcoal treatment for the removal of furans and phenols was reported in the detoxification of olive tree biomass [19], sweet sorghum bagasse [29], palm press fiber [30], Agave lechuguilla [31], and brewers' spent grain [32].…”

Section: Composition and Detoxification Of Eop Hydrolysatementioning

confidence: 97%

Production of Ethanol from Hemicellulosic Sugars of Exhausted Olive Pomace by Escherichia coli

et al. 2020

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Exhausted olive pomace (EOP) is the main residue generated in olive oil industries, after the extraction of the residual oil from olive pomace with hexane. This work studies the ethanol production from hemicellulosic sugars of EOP. The fermentability of the sugar solution, resulting from the acid pretreatment of EOP, was evaluated using Escherichia coli SL100, although a detoxification step was required before fermentation. Overliming and activated charcoal detoxification were tested to minimize the presence of inhibitory compounds in the hydrolysate and to achieve a fermentable medium. E. coli assimilated all sugars in both detoxified hydrolysates and achieved ethanol yields of about 90% of the theoretical one. However, the fermentation time was much shorter when the hydrolysate had been detoxified with activated charcoal (20 h versus 120 h).

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Optimization of dilute acid pretreatment of Agave lechuguilla and ethanol production by co-fermentation with Escherichia coli MM160

Cited by 46 publications

References 48 publications

Valorisation of Exhausted Olive Pomace by an Eco-Friendly Solvent Extraction Process of Natural Antioxidants

Valorisation of Exhausted Olive Pomace by an Eco-Friendly Solvent Extraction Process of Natural Antioxidants

High Throughput Profiling of Flavonoid Abundance in Agave lechuguilla Residue-Valorizing under Explored Mexican Plant

Production of Ethanol from Hemicellulosic Sugars of Exhausted Olive Pomace by Escherichia coli

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