Optimization of Enzymatic Hydrolysis of Sacha Inchi Oil using Conventional and Supercritical Carbon Dioxide Processes

Prado, Glaucia H. C.; Saldaña, Marleny D.A.

doi:10.1007/s11746-013-2205-4

Cited by 9 publications

(8 citation statements)

References 37 publications

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“…These results showed that enough water is necessary for the hydrolysis of Phoenix tree seed oil. Similar effect of water on the lipase-catalyzed hydrolysis of oil can also be found in previous reports (Chen et al, 2014;Prado and Saldaña, 2013).…”

Section: Effect Of Mass Ratio Of Water To Oilsupporting

confidence: 88%

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Enzymatic production of sterculic acid from the novel Phoenix tree seed oil: Optimization and kinetic study

Hou

Sun

2017

Grasas y Aceites

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SUMMARY:Phoenix tree (Firmiana simplex) seed oil is a novel oil which is rich in sterculic acid. Sterculic acid, a cyclopropene fatty acid, can be used as the inhibitor of the stearoyl-CoA desaturase system and mammary carcinomas growth. In this work, Lipozyme TLIM-catalyzed hydrolysis of the novel Phoenix tree seed oil was used to prepare sterculic acid. High temperature GC-FID and the degree of hydrolysis (DH) were used to monitor the reaction progress. Effects of reaction variables on the hydrolysis were evaluated and optimized using response surface methodology. Results showed that sterculic acid can be successfully prepared from the novel seed oil, and the effect of reaction variables on the hydrolysis decreased in the order of reaction time > enzyme load > temperature. RESUMEN: Producción enzimática de ácido estercúlico a partir del nuevo aceite de semillas del árbol fenix: optimización y estudio cinético. El aceite de semilla del árbol fenix (Firmiana simplex) es un nuevo tipo de aceite rico en ácido estercúlico. Este es un ácido graso ciclopropeno, que se puede utilizar como inhibidor del sistema de estearoyl-CoA desaturasa y del crecimiento de los carcinomas mamarios. En este trabajo, se utilizó la hidrólisis catalizada por Lipozyme TLIM del nuevo aceite de semilla de árbol fenix para preparar ácido estercúlico. Se utilizaron GC-FID a alta temperatura y el grado de hidrólisis (DH) para monitorizar el progreso de la reacción. Los efectos de las variables de reacción de hidrólisis se evaluaron y optimizaron utilizando la metodología de superficie de respuesta. Los resultados mostraron que el ácido estercúlico puede prepararse con éxito a partir del nuevo aceite de semilla, y el efecto de las variables de reacción en la hidrólisis disminuyó en el orden de tiempo de reacción> carga enzimática> temperatura. Se pueden obtener altos rendimientos de ácidos grasos (DH, 98,2 ± 0,8%) en condiciones optimizadas (45 ºC, relación masa de agua a aceite 10: 1, carga enzimática 10% y 18 h). La ecuación de Arrhenius para la hidrólisis fue LnV 0 = 9,12 − 4721/T. La energía de activación fue 39,25KJ/mol. Los valores cinéticos de Vmax, K/m fueron 0,232 mol/(L • min) y 0,084 mol/L, respectivamente. Citation/Cómo citar este artículo: Hou X, Sun S. 2017. Enzymatic production of sterculic acid from the novel Phoenix tree seed oil: Optimization and kinetic study. Grasas Aceites 68 (2), e197. http://dx

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Section: Effect Of Mass Ratio Of Water To Oilsupporting

confidence: 88%

“…To evaluate the effect of the reaction temperature on the hydrolysis of Phoenix tree seed oil, experiments were performed at different temperatures from (Prado and Saldaña, 2013), which was attributed to the difference in hydrolysis media.…”

Section: Effect Of Reaction Temperaturementioning

confidence: 99%

Enzymatic production of sterculic acid from the novel Phoenix tree seed oil: Optimization and kinetic study

Hou

Sun

2017

Grasas y Aceites

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“…Although this conventional method produces high conversion yield, it requires high temperature (100–260 °C) and pressure (100–7000 kPa), thus causing undesirable reactions such as dehydration, oxidation of fatty acids, and interesterification of triglyceride . Oil hydrolysis using lipase as a biocatalyst has received considerable attention for the production of fatty acids . This method is more advantageous than the conventional method because the method can be employed at low temperatures and demonstrates high selectivity, thus leading to energy conservation and obtaining high‐purity products .…”

Section: Introductionmentioning

confidence: 99%

“…[13][14][15] Oil hydrolysis using lipase as a biocatalyst has received considerable attention for the production of fatty acids. [16][17][18] This method is more advantageous than the conventional method because the method can be employed at low temperatures and demonstrates high selectivity, thus leading to energy conservation and obtaining high-purity products. 17,19,20 In addition, the use of lipase for oil hydrolysis guarantees a contamination-free product, which is the primary concern in food technology, cosmetics, and biomedical applications, because consumers prefer natural compounds or those that are identical to natural compounds rather than chemically synthetic products.…”

Section: Introductionmentioning

confidence: 99%

Liquid lipase‐catalyzed hydrolysis of gac oil for fatty acid production: Optimization using response surface methodology

Nguyen

et al. 2018

Biotechnology Progress

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Fatty acids are valuable products because they have wide industrial applications in the manufacture of detergents, cosmetics, food, and various biomedical applications. In enzyme‐catalyzed hydrolysis, the use of immobilized lipase results in high production cost. To address this problem, Eversa Transform lipase, a new and low‐cost liquid lipase formulation, was used for the first time in oil hydrolysis with gac oil as a triglyceride source in this study. Response surface methodology was employed to optimize the reaction conditions and establish a reliable mathematical model for predicting hydrolysis yield. A maximal yield of 94.16% was obtained at a water‐to‐oil molar ratio of 12.79:1, reaction temperature of 38.9 °C, enzyme loading of 13.88%, and reaction time of 8.41 h. Under this optimal reaction condition, Eversa Transform lipase could be reused for up to eight cycles without significant loss in enzyme activity. This study indicates that the use of liquid Eversa Transform lipase in enzyme‐catalyzed oil hydrolysis could be a promising and cheap method of fatty acid production. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 2018

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“…Sacha inchi oil has high content of unsaturated fatty acids, mainly linoleic acid, and a-linolenic acid. These fatty acids are essential fatty acids, which mean that humans cannot synthesize them and they should be acquired from the diet (Prado & Saldaña, 2013). Its oil contain more than 90% of unsaturated fatty acids including:42-48% linolenic acid (x-3), 32-37% linoleic acid (x-6), and 9-12% oleic acid (x-9) (Chirinos, Pedreschi, Domínguez, & Campos, 2015a;Follegatti-Romero, Piantino, Grimaldi, & Cabral, 2009); the ratio of 0.76:1 for omega-6:omega-3 is good for human health (Prado et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Enhanced antioxidant properties of tara (Caesalpinia spinosa) gallotannins by thermal hydrolysis and its synergistic effects with α‐tocopherol, ascorbyl palmitate, and citric acid on sacha inchi (Plukenetia volubilis) oil

Hilares

Chirinos

Pedreschi

et al. 2017

J Food Process Engineering

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The antioxidant potential of thermally hydrolyzed tara pod extracts (HTEs) at 121 °C, 103 kPa for different time were tested against sacha inchi oil (SIO) oxidation. The antioxidant efficacy on SIO of 200–400 ppm HTE for 180 min were compared to α‐tocopherol (α‐T), citric acid (CA) and ascorbyl palmitate (AP). Additionally, synergistic or antagonistic effects in binary mixtures of HTE with α‐T or CA or AP were tested. Accelerated storage tests (55 °C for 30 days) were used to evaluate peroxide (PV), p‐anisidine values (p‐AV) and stability of naturally occurring SIO tocopherols and phytosterols. Antioxidant capacity (AC) and the antioxidant efficacy on SIO significantly increased as the HTE hydrolysis degree (HD) increased. The mixture of 400 ppm HTE and 100 ppm AP displayed a synergistic effect and reduced the rate of peroxide formation, p‐AV and better protected SIO tocopherols. HTEs protect oils due to their high AC being an interesting alternative as natural antioxidant. Practical applications Tara pods are an important source of gallotannins with antioxidant and antimicrobial properties widely used by the pharmaceutical, chemical, painting, and tannery industries. The partial thermal hydrolysis of gallotannins results in an increment of antioxidant activity determined in vitro by the ABTS, FRAP, and ORAC assays and to increase the efficacy against oxidation of sacha inchi oil (a food matrix rich in polyunsaturated fatty acids). These results demonstrate that partially hydrolyzed gallotannins with a high antioxidant activity are an important alternative for the functional and nutraceutical industries.

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Optimization of Enzymatic Hydrolysis of Sacha Inchi Oil using Conventional and Supercritical Carbon Dioxide Processes

Cited by 9 publications

References 37 publications

Enzymatic production of sterculic acid from the novel Phoenix tree seed oil: Optimization and kinetic study

Enzymatic production of sterculic acid from the novel Phoenix tree seed oil: Optimization and kinetic study

Liquid lipase‐catalyzed hydrolysis of gac oil for fatty acid production: Optimization using response surface methodology

Enhanced antioxidant properties of tara (Caesalpinia spinosa) gallotannins by thermal hydrolysis and its synergistic effects with α‐tocopherol, ascorbyl palmitate, and citric acid on sacha inchi (Plukenetia volubilis) oil

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