Optimization of enzymatic hydrolysis of bamboo biomass for enhanced saccharification of cellulose through Taguchi orthogonal design

Chavan, Shivanand; Gaikwad, Ashwin

doi:10.1016/j.jece.2020.104807

Cited by 23 publications

(12 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Temperature is an important factor affecting enzyme activity [ 11 , 34 ]. The effects of hydrolysis temperature on the antioxidant activity of GDP in vitro are displayed in Figure S3 .…”

Section: Resultsmentioning

confidence: 99%

Enzymatic Degradation of Gracilariopsis lemaneiformis Polysaccharide and the Antioxidant Activity of Its Degradation Products

Tian

Zhang

et al. 2021

Marine Drugs

View full text Add to dashboard Cite

Gracilariopsis lemaneiformis polysaccharides (GLP) were degraded using pectinase, glucoamylase, cellulase, xylanase, and β-dextranase into low-molecular-weight polysaccharides, namely, GPP, GGP, GCP, GXP, and GDP, respectively, and their antioxidant capacities were investigated. The degraded GLPs showed higher antioxidant activities than natural GLP, and GDP exhibited the highest antioxidant activity. After the optimization of degradation conditions through single-factor and orthogonal optimization experiments, four polysaccharide fractions (GDP1, GDP2, GDP3, and GDP4) with high antioxidant abilities (hydroxyl radical scavenging activity, DPPH radical scavenging activity, reduction capacity, and total antioxidant capacity) were obtained. Their cytoprotective activities against H2O2-induced oxidative damage in human fetal lung fibroblast 1 (HFL1) cells were examined. Results suggested that GDP pretreatment can significantly improve cell viability, reduce reactive oxygen species and malonaldehyde levels, improve antioxidant enzyme activity and mitochondria membrane potential, and alleviate oxidative damage in HFL1 cells. Thus, the enzyme degradation of GLP with β-dextranase can significantly improve its antioxidant activity, and GDP might be a suitable source of natural antioxidants.

show abstract

“…Temperature is an important factor affecting enzyme activity [ 11 , 34 ]. The effects of hydrolysis temperature on the antioxidant activity of GDP in vitro are displayed in Figure S3 .…”

Section: Resultsmentioning

confidence: 99%

Enzymatic Degradation of Gracilariopsis lemaneiformis Polysaccharide and the Antioxidant Activity of Its Degradation Products

Tian

Zhang

et al. 2021

Marine Drugs

View full text Add to dashboard Cite

show abstract

“…The kinetic energy of reacting molecules increases with the temperature rise, resulting in a higher collision rate and subsequent substrate conversion into a product. However, at elevated temperature, water that critically affects protein folding and structure are lost, due to which enzyme activity is compromised ( Chavan and Gaikwad, 2021 ). The deviations were possibly due to the difference in the substrate used in enzyme assay and saccharification, as cellulases exhibit differential specificity and affinity for soluble and insoluble substrates ( Sidar et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“…Cellulases are commonly used to refer to the three enzymes that convert cellulose into glucose (fermentable monosaccharide): endocellulase, exocellulase, and glucosidase ( Zhao et al, 2019 ). Different physicochemical parameters affect hydrolysis efficiency, such as incubation temperature, pH, agitation speed/rpm, incubation time, enzyme/substrate ratio, and particle size ( Chavan and Gaikwad, 2021 ; Faizal et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Sustainable Production of Bioethanol Using Levulinic Acid Pretreated Sawdust

Nawaz

Huang

Junaid

et al. 2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

The sustainability and economic viability of the bioethanol production process from lignocellulosic biomass depend on efficient and effective pretreatment of biomass. Traditional pretreatment strategies implicating the use of mineral acids, alkalis, and organic solvents release toxic effluents and the formation of inhibitory compounds posing detrimental effects on the environment and interfering with the enzymatic saccharification process, respectively. Ionic liquids (ILs) as green solvents were used to overcome this issue, but the deep eutectic solvent as an emerging class of ionic liquids performed better in terms of making the process environmentally and economically viable. The green solvent-based pretreatment strategy applied in the current research was levulinic, acid-based natural deep eutectic solvent (NADES). Three different hydrogen bond acceptors (HBAs)—acetamide, betaine, and choline chloride—in combination with levulinic acid as hydrogen bond donor (HBD) in (HBD: HBA) molar ratio 2:1, were screened for biomass pretreatment. The best deep eutectic solvent was levulinic acid: choline chloride in an optimized molar ratio of 1:0.5, resulting in 91% delignification. The physicochemical parametric optimization of saccharification exhibited maximum enzymatic hydrolysis of 25.87% with 125 mg of pretreated sawdust via simultaneous addition of three thermostable cellulases [i.e., endo-1,4-β-D-glucanase (240 U), exo-1,4-β-D-glucanase (180 U), and β-glucosidase (320 U)] for 5 h of incubation at 75°C. The reducing sugar slurry obtained from the saccharified biomass was then added to a fermentation medium for bioethanol production, and a maximum of 11.82% of production was obtained at 30°C, 72 h, and 180 rpm using a 2.5% 24 h old Saccharomyces cerevisiae seed culture. The current study revealed that the levulinic-based deep eutectic solvent exhibited remarkable delignification, which led to the efficient enzymatic hydrolysis of sawdust and hence bioethanol production. Furthermore, it will prospect new avenues in bioethanol production using a deep eutectic solvent. Deep eutectic solvent overcame the issues posed by ionic liquids: toxicity, expensive and complex preparation, and non-biodegradability.

show abstract

“…First- and second-generation biofuels are obtained mainly from renewable plant materials. Food crops, e.g., wheat [ 4 ], corn [ 5 ], sugarcane [ 6 ], etc., are the main materials for first-generation biofuels, while lignocellulosic materials, e.g., inexpensive wastes of food and non-food industries, can be used for the biochemical processing into second-generation biofuels [ 7 , 8 ]. The growing interest in the development and application of new goods and materials, chemicals of an aromatic nature, and energy and fuels from renewable vegetable wastes can be observed worldwide [ 9 , 10 , 11 ] due to their widespread availability and low cost.…”

Section: Introductionmentioning

confidence: 99%

Apricot Seed Shells and Walnut Shells as Unconventional Sugars and Lignin Sources

et al. 2023

View full text Add to dashboard Cite

The present study focuses on using apricot seeds shells and walnut shells as a potential renewable material for biorefinery in Ukraine. The goal of the research work was to determine the relationship between the chemical composition of solid residues from biomass after acid pretreatment with H2SO4, alkaline pretreatment with NaOH, and a steam explosion pretreatment and the recovery of sugars and lignin after further enzymatic hydrolysis with the application of an industrial cellulase Cellic CTec2. Apricot seeds shells and walnut shells consist of lots of cellulose (35.01 and 24.19%, respectively), lignin (44.55% and 44.63%, respectively), hemicelluloses (10.77% and 26.68%, respectively), and extractives (9.97% and 11.41%, respectively), which affect the efficiency of the bioconversion of polysaccharides to sugars. The alkaline pretreatment was found to be more efficient in terms of glucose yield in comparison with that of acid and steam explosion, and the maximum enzymatic conversions of cellulose reached were 99.7% and 94.6% for the solids from the apricot seeds shells and the walnut shells, respectively. The maximum amount of lignin (82%) in the residual solid was obtained during the processing of apricot seed shells submitted to the acid pretreatment. The amount of lignin in the solids interferes with the efficiency of enzymatic hydrolysis. The results pave the way for the efficient and perspective utilization of shells through the use of inexpensive, simple and affordable chemical technologies, obtaining value-added products, and thus, reducing the amount of environmental pollution (compared to the usual disposal practice of direct burning) and energy and material external dependency (by taking advantage of these renewable, low-cost materials).

show abstract

Optimization of enzymatic hydrolysis of bamboo biomass for enhanced saccharification of cellulose through Taguchi orthogonal design

Cited by 23 publications

References 38 publications

Enzymatic Degradation of Gracilariopsis lemaneiformis Polysaccharide and the Antioxidant Activity of Its Degradation Products

Enzymatic Degradation of Gracilariopsis lemaneiformis Polysaccharide and the Antioxidant Activity of Its Degradation Products

Sustainable Production of Bioethanol Using Levulinic Acid Pretreated Sawdust

Apricot Seed Shells and Walnut Shells as Unconventional Sugars and Lignin Sources

Contact Info

Product

Resources

About