Optimization of enzyme-assisted extraction of polysaccharides from alfalfa and its antioxidant activity

Wang, Shaopu; Dong, Xiaofang; Tong, Jun

doi:10.1016/j.ijbiomac.2013.09.029

Cited by 86 publications

(51 citation statements)

References 35 publications

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“…The quadratic effect of all the three variables was significant in affecting TPC and TFC. The similar effect of time in enzymatic hydrolysis is earlier reported in extraction of reducing sugars from banana peel (Oberoi, Sandhu, & Vadlani, 2012) and polysaccharides from alfalfa (Wang, Dong, & Tong, 2013). Moreover, Zuorro, Fidaleo, and Lavecchia (2011) also reported the effect of time, enzyme concentration, and SS ratio on extraction yield of lycopene form tomato processing waste.…”

Section: Eaesupporting

confidence: 81%

Enzyme‐ and ultrasound‐assisted extractions of polyphenols from Citrus sinensis (cv. Malta) peel: A comparative study

Nishad

Saha

Kaur

2019

J Food Process Preserv

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Response surface methodology (RSM) was employed for optimization of ultrasound‐assisted extraction (UAE) and enzyme‐assisted extraction (EAE) of bioactives from Citrus sinensis cv. Malta peel. The optimized extract was further compared with conventional solvent extraction for total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activity (AOX). The optimized condition for UAE was obtained at 70.89% amplitude, 40 ml/g solvent–solid (SS) ratio, and an extraction time of 35 min. Moreover, the optimized condition for EAE was 0.84% enzyme concentration, 30.94 ml/g SS ratio and 4.87 hr extraction time. The predicted TPC and TFC recoveries under the optimized conditions were significantly similar to the experimental values, indicating suitability of the employed RSM model. Phenolic profiling through HPLC confirmed the effect of extraction methods on released phenolics and AOX. EAE enhanced twofold higher yield of phenolics than UAE. Thus, it seems EAE has significant industrial implications in valorization of the waste from horticultural produce. Practical applications In the present research, we have optimized the process for extraction of bioactives using enzyme‐ and ultrasound‐assisted methods from citrus peel. Based on high recovery of phenolics and antioxidant activity, enzyme‐assisted process was found to be more efficient than ultrasound. Results suggest that enzyme‐assisted extraction process may have promising applications for processing of industrial byproducts and reducing environmental waste.

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Section: Eaesupporting

confidence: 81%

Enzyme‐ and ultrasound‐assisted extractions of polyphenols from Citrus sinensis (cv. Malta) peel: A comparative study

Nishad

Saha

Kaur

2019

J Food Process Preserv

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“…First, higher extraction temperatures can promote mass transfer and accelerate the extraction speed, while excessively high temperatures may lead to the thermo-degradation of the polysaccharides. Second, enzymes have a maximal hydrolyzing activity at their optimum temperature, which significantly affects the enzyme activities [23]. A mixture of enzymes has an optimum temperature at which each component has optimal activity.…”

Section: Effect Of Temperature On Extraction Yield Of Polysaccharidesmentioning

confidence: 99%

Extraction, purification and anti-proliferative activities of polysaccharides from Lentinus edodes

Zhao

Jin

et al. 2016

International Journal of Biological Macromolecules

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“…The radical-scavenging ability of DPPH was measured according to the method of [54]. Thus, the DPPH radical solution was produced by gently mixing 0.1 mM DPPH solution (0.75 mL) and the sample solution (1.5 mL) with various concentrations.…”

Section: Dpph Radical Scavenging Capacity Measurementmentioning

confidence: 99%

Optimization of Ultrasonic-Assisted Extraction of Flavonoid Compounds and Antioxidants from Alfalfa Using Response Surface Method

2015

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Ultrasonic-assisted extraction (UAE) was used to extract flavonoid-enriched antioxidants from alfalfa aerial part. Response surface methodology (RSM), based on a four-factor, five-level central composite design (CCD), was employed to obtain the optimal extraction parameters, in which the flavonoid content was maximum and the antioxidant activity of the extracts was strongest. Radical scavenging capacity of the extracts, which represents the amounts of antioxidants in alfalfa, was determined by using 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonicacid) (ABTS) and 2,2′-diphenyl-1-picrylhydrazyl (DPPH) methods. The results showed good fit with the proposed models for the total flavonoid extraction (R 2 = 0.9849), for the antioxidant extraction assayed by ABTS method (R 2 = 0.9764), and by DPPH method (R 2 = 0.9806). Optimized extraction conditions for total flavonoids was a ratio of liquid to solid of 57.16 mL/g, 62.33 °C, 57.08 min, and 52.14% ethanol. The optimal extraction parameters of extracts for the highest antioxidant activity by DPPH method was a ratio of liquid to solid 60.3 mL/g, 54.56 °C, 45.59 min, and 46.67% ethanol, and by ABTS assay was a ratio of liquid to solid 47.29 mL/g, 63.73 °C, 51.62 min, and 60% ethanol concentration. Our work offers optimal extraction conditions for total flavonoids and antioxidants from alfalfa.

show abstract

Optimization of enzyme-assisted extraction of polysaccharides from alfalfa and its antioxidant activity

Cited by 86 publications

References 35 publications

Enzyme‐ and ultrasound‐assisted extractions of polyphenols from Citrus sinensis (cv. Malta) peel: A comparative study

Enzyme‐ and ultrasound‐assisted extractions of polyphenols from Citrus sinensis (cv. Malta) peel: A comparative study

Extraction, purification and anti-proliferative activities of polysaccharides from Lentinus edodes

Optimization of Ultrasonic-Assisted Extraction of Flavonoid Compounds and Antioxidants from Alfalfa Using Response Surface Method

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