Optimization of enzyme immobilization on functionalized magnetic nanoparticles for laccase biocatalytic reactions

Fortes, Cristiano; Daniel‐da‐Silva, Ana L.; Xavier, Ana M. R. B.; Tavares, Ana P. M.

doi:10.1016/j.cep.2017.03.009

Cited by 136 publications

(45 citation statements)

References 143 publications

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“…Among the described works, maltose-functionalised magnetic core/shell Fe 3 O 4 @Au NPs [ 74 ] seem to be the most promising nanomaterial for ASNase confinement by physical adsorption due to the highest η i displayed (77.2%), along with the high TS (90% of activity after 3 h of incubation at 55 °C) and SS (64% of activity following 28 days at 25 °C), ability to keep half of its initial enzymatic activity after 13 cycles of reaction (OS) and enhanced confined ASNase affinity. The combination of (nano)materials with magnetic properties allows for an easy separation process [ 22 , 110 , 111 ], providing a good option for the ASNase confinement.…”

Section: Types Of Asnase Confinementmentioning

confidence: 99%

Recent Strategies and Applications for l-Asparaginase Confinement

et al. 2020

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l-asparaginase (ASNase, EC 3.5.1.1) is an aminohydrolase enzyme with important uses in the therapeutic/pharmaceutical and food industries. Its main applications are as an anticancer drug, mostly for acute lymphoblastic leukaemia (ALL) treatment, and in acrylamide reduction when starch-rich foods are cooked at temperatures above 100 °C. Its use as a biosensor for asparagine in both industries has also been reported. However, there are certain challenges associated with ASNase applications. Depending on the ASNase source, the major challenges of its pharmaceutical application are the hypersensitivity reactions that it causes in ALL patients and its short half-life and fast plasma clearance in the blood system by native proteases. In addition, ASNase is generally unstable and it is a thermolabile enzyme, which also hinders its application in the food sector. These drawbacks have been overcome by the ASNase confinement in different (nano)materials through distinct techniques, such as physical adsorption, covalent attachment and entrapment. Overall, this review describes the most recent strategies reported for ASNase confinement in numerous (nano)materials, highlighting its improved properties, especially specificity, half-life enhancement and thermal and operational stability improvement, allowing its reuse, increased proteolysis resistance and immunogenicity elimination. The most recent applications of confined ASNase in nanomaterials are reviewed for the first time, simultaneously providing prospects in the described fields of application.

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Section: Types Of Asnase Confinementmentioning

confidence: 99%

Recent Strategies and Applications for l-Asparaginase Confinement

et al. 2020

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“…Several methods have also been reported for enzyme immobilization by using different nanomaterials [128,129,130,131,132,133]. Functionalized nanomaterials promote interactions between the electrode surface and the enzyme active center by means of increased electroactive surface area, improving sensitivity and electron transfer kinetics.…”

Section: Enzyme Sensorsmentioning

confidence: 99%

Latest Trends in Electrochemical Sensors for Neurotransmitters: A Review

Tavakolian-Ardakani

Hosu

Cristea

et al. 2019

Sensors

117

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Neurotransmitters are endogenous chemical messengers which play an important role in many of the brain functions, abnormal levels being correlated with physical, psychotic and neurodegenerative diseases such as Alzheimer's, Parkinson's, and Huntington's disease. Therefore, their sensitive and robust detection is of great clinical significance. Electrochemical methods have been intensively used in the last decades for neurotransmitter detection, outclassing more complicated analytical techniques such as conventional spectrophotometry, chromatography, fluorescence, flow injection, and capillary electrophoresis. In this manuscript, the most successful and promising electrochemical enzyme-free and enzymatic sensors for neurotransmitter detection are reviewed. Focusing on the activity of worldwide researchers mainly during the last ten years (2010–2019), without pretending to be exhaustive, we present an overview of the progress made in sensing strategies during this time. Particular emphasis is placed on nanostructured-based sensors, which show a substantial improvement of the analytical performances. This review also examines the progress made in biosensors for neurotransmitter measurements in vitro, in vivo and ex vivo.

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“…The developed alkaline protease nanobiocatalyst exhibited significant enhancement in thermostability, catalytic activity, substrate affinity and stability in a variety of organic solvents, surfactants and commercial laundry detergents. Immobilization of several enzyme into nanomaterial have been reported recently, showing significant improvement of enzymes properties including lipase/mesoporous silica (Zheng et al, 2017), laccase/magnetic nanoparticles (Fortes et al, 2017), pectinase/chitosan magnetic nanoparticles (Sojitra et al, 2017), α-amylase/ metallic/bimetallic magnetic nanoparticle , transaminase/PVA-Fe 3 O 4 nanoparticle (Jia et al, 2016), trypsin/magnetic nanofibers and others (Lee et al, 2016).…”

Section: Nanostructured Materials and Nanobiocatalysismentioning

confidence: 99%

Recent Trends for Discovery and Enhancement of Enzyme Function: A Review

Ibrahim

El‐Dewany

2018

Egypt. J. Microbiol.

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R ECENTLY, there is a growing recognition of enzymes utilization as ecofriendly alternative for chemical catalysts in various industrial sectors. Two main approaches are used to expand the enzymes utilization in various industrial applications including (i) Searching nature for discovery of better performing novel biocatalysts and (ii) Improvement of the enzyme function and properties. The recent advances in biocatalyst discovery and engineering have led to an increase of the efficiency and functional diversityof enzymes. Therefore, enzyme application is expanding to broader industrial processes, including those which were previously restricted to the classical chemical catalysts. Furthermore, there is a significant success in developing made-to-order enzymes that meet the industrial process conditions requirements, leading to more efficient and cost-effective processes. Here I highlight state-of-the-art strategies and tools for novel enzyme discovery and function enhancement, including enzyme engineering at the molecular level through different approaches such as directed evolution, rational design, semi rational design and computational de novo enzyme design; in addition to the recently developed field, nanobiocatalysis.

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Optimization of enzyme immobilization on functionalized magnetic nanoparticles for laccase biocatalytic reactions

Cited by 136 publications

References 143 publications

Recent Strategies and Applications for l-Asparaginase Confinement

Recent Strategies and Applications for l-Asparaginase Confinement

Latest Trends in Electrochemical Sensors for Neurotransmitters: A Review

Recent Trends for Discovery and Enhancement of Enzyme Function: A Review

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